scholarly journals Differential temporal effects of sclerostin antibody and parathyroid hormone on cancellous and cortical bone and quantitative differences in effects on the osteoblast lineage in young intact rats

Bone ◽  
2015 ◽  
Vol 81 ◽  
pp. 380-391 ◽  
Author(s):  
Michael S. Ominsky ◽  
Danielle L. Brown ◽  
Gwyneth Van ◽  
David Cordover ◽  
Efrain Pacheco ◽  
...  
Keyword(s):  
Parathyroid Hormone ◽  
Cortical Bone ◽  
Temporal Effects ◽  
Sclerostin Antibody ◽  
Osteoblast Lineage ◽  
Intact Rats

scholarly journals Gender differences in the response of CD-1 mouse bone to parathyroid hormone: potential role of IGF-I

2006 ◽  
Vol 189 (2) ◽  
pp. 279-287 ◽  
Author(s):  
Yongmei Wang ◽  
Takeshi Sakata ◽  
Hashem Z Elalieh ◽  
Scott J Munson ◽  
Andrew Burghardt ◽  
...  
Keyword(s):  
Gender Differences ◽  
Parathyroid Hormone ◽  
Cortical Bone ◽  
Mineral Apposition Rate ◽  
Mrna Levels ◽  
Male Mice ◽  
Bone Formation Rate ◽  
Male And Female ◽  
Female Mice ◽  
Igf I

Parathyroid hormone (PTH) exerts both catabolic and anabolic actions on bone. Studies on the skeletal effects of PTH have seldom considered the effects of gender. Our study was designed to determine whether the response of mouse bone to PTH differed according to sex. As a first step, we analyzed gender differences with respect to bone mass and structural properties of 4 month old PTH treated (80 μg/kg per day for 2 weeks) male and female CD-1 mice. PTH significantly increased fat free weight/body weight, periosteal bone formation rate, mineral apposition rate, and endosteal single labeling surface, while significantly decreasing medullary area in male mice compared with vehicle treated controls, but induced no significant changes in female mice. We then analyzed the gender differences in bone marrow stromal cells (BMSC) isolated from 4 month old male and female CD-1 mice following treatment with PTH (80 μg/kg per day for 2 weeks). PTH significantly increased the osteogenic colony number and the alkaline phosphatase (ALP) activity (ALP/cell) by day 14 in cultures of BMSCs from male and female mice. PTH also increased the mRNA level of receptor activator of nuclear factor κB ligand in the bone tissue (marrow removed) of both females and males. However, PTH increased the mRNA levels of IGF-I and IGF-IR only in the bones of male mice. Our results indicate that on balance a 2-weeks course of PTH is anabolic on cortical bone in this mouse strain. These effects are more evident in the male mouse. These differences between male and female mice may reflect the greater response to PTH of IGF-I and IGF-IR gene expression in males enhancing the anabolic effect on cortical bone.

Erratum to “Mechanical loading enhances the anabolic effects of intermittent parathyroid hormone (1–34) on trabecular and cortical bone in mice” [Bone 43 (2008) 238–248]

Bone ◽  
2009 ◽  
Vol 45 (6) ◽  
pp. 1192-1195
Author(s):  
Toshihiro Sugiyama ◽  
Leanne K. Saxon ◽  
Gul Zaman ◽  
Alaa Moustafa ◽  
Andrew Sunters ◽  
...  
Keyword(s):  
Parathyroid Hormone ◽  
Cortical Bone ◽  
Mechanical Loading

scholarly journals Prostaglandin E2 (PGE2) and Risedronate Was Superior to PGE2 Alone in Maintaining Newly Added Bone in the Cortical Bone Site After Withdrawal in Older Intact Rats

1997 ◽  
Vol 12 (2) ◽  
pp. 267-275 ◽  
Author(s):  
Yan Fei Ma ◽  
Bai Yun Lin ◽  
Webster S. S. Jee ◽  
Chao Hua Lin ◽  
Yong Yong Chen ◽  
...  
Keyword(s):  
Prostaglandin E2 ◽  
Cortical Bone ◽  
Intact Rats

Bioactive parathyroid hormone in pregnant rats and fetuses

1990 ◽  
Vol 258 (4) ◽  
pp. E549-E554 ◽  
Author(s):  
A. Bourdeau ◽  
G. Manganella ◽  
C. L. Thil-Trubert ◽  
C. Sachs ◽  
G. Cournot
Keyword(s):  
Parathyroid Hormone ◽  
Plasma Calcium ◽  
Ether Anesthesia ◽  
Pregnant Rats ◽  
Calcium Gradient ◽  
Osteoclast Number ◽  
Cytochemical Bioassay ◽  
Parathyroid Function ◽  
Immunoreactive Parathyroid Hormone ◽  
Intact Rats

Parathyroid function at the end of gestation (day 21) was investigated by measuring plasma calcium (PCa), immunoreactive parathyroid hormone (iPTH), bioactive parathyroid hormone (bioPTH; cytochemical bioassay), and bone histology in intact and thyroparathyroidectomized (TPTX; day 12, ether anesthesia) rats and their fetuses. In pregnant intact rats, PCa was significantly lower, and iPTH, bioPTH, and osteoclast number were higher than in nonpregnant rats. In fetuses, PCa was higher than maternal PCa and correlated with fetal bioPTH. TPTX suppressed maternal bioPTH and decreased iPTH and osteoclast number, whereas fetal iPTH and bioPTH were decreased with no change in osteoclast number. Fetal PCa was near normal and was correlated with maternal PCa but not with fetal bioPTH. The fetomaternal calcium gradient was maintained and even increased. This study shows that there is maternal physiological hyperparathyroidism and functional fetal parathyroid glands at the end of gestation in the rat. Parathyroid hormone does not seem to be responsible for maintaining the high fetomaternal calcium gradient in TPTX animals.

scholarly journals EphrinB2/EphB4 inhibition in the osteoblast lineage modifies the anabolic response to parathyroid hormone

2013 ◽  
Vol 28 (4) ◽  
pp. 912-925 ◽  
Author(s):  
Farzin M Takyar ◽  
Stephen Tonna ◽  
Patricia WM Ho ◽  
Blessing Crimeen-Irwin ◽  
Emma K Baker ◽  
...  
Keyword(s):  
Parathyroid Hormone ◽  
Anabolic Response ◽  
Osteoblast Lineage

scholarly journals Loss of Gsα in the Postnatal Skeleton Leads to Low Bone Mass and a Blunted Response to Anabolic Parathyroid Hormone Therapy

2015 ◽  
Vol 291 (4) ◽  
pp. 1631-1642 ◽  
Author(s):  
Partha Sinha ◽  
Piia Aarnisalo ◽  
Rhiannon Chubb ◽  
Ingrid J. Poulton ◽  
Jun Guo ◽  
...  
Keyword(s):  
Parathyroid Hormone ◽  
Bone Formation ◽  
Bone Mass ◽  
Trabecular Bone ◽  
Phospholipase C ◽  
Osteoblast Differentiation ◽  
Bone Volume ◽  
Trabecular Bone Volume ◽  
Male And Female ◽  
Osteoblast Lineage

Parathyroid hormone (PTH) is an important regulator of osteoblast function and is the only anabolic therapy currently approved for treatment of osteoporosis. The PTH receptor (PTH1R) is a G protein-coupled receptor that signals via multiple G proteins including Gsα. Mice expressing a constitutively active mutant PTH1R exhibited a dramatic increase in trabecular bone that was dependent upon expression of Gsα in the osteoblast lineage. Postnatal removal of Gsα in the osteoblast lineage (P-GsαOsxKO mice) yielded markedly reduced trabecular and cortical bone mass. Treatment with anabolic PTH(1–34) (80 μg/kg/day) for 4 weeks failed to increase trabecular bone volume or cortical thickness in male and female P-GsαOsxKO mice. Surprisingly, in both male and female mice, PTH administration significantly increased osteoblast numbers and bone formation rate in both control and P-GsαOsxKO mice. In mice that express a mutated PTH1R that activates adenylyl cyclase and protein kinase A (PKA) via Gsα but not phospholipase C via Gq/11 (D/D mice), PTH significantly enhanced bone formation, indicating that phospholipase C activation is not required for increased bone turnover in response to PTH. Therefore, although the anabolic effect of intermittent PTH treatment on trabecular bone volume is blunted by deletion of Gsα in osteoblasts, PTH can stimulate osteoblast differentiation and bone formation. Together these findings suggest that alternative signaling pathways beyond Gsα and Gq/11 act downstream of PTH on osteoblast differentiation.

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