Utilizing time-lapse micro-CT-correlated bisphosphonate binding kinetics and soft tissue-derived input functions to differentiate site-specific changes in bone metabolism in vivo

Utilizing bisphosphonate binding kinetics and soft tissue-derived input functions to differentiate changes in long bone and vertebra bone metabolism using in vivo fluorescent molecular tomography

Bone Abstracts ◽

10.1530/boneabs.3.pp225 ◽

2014 ◽

Author(s):

Robert J Tower ◽

Marc Muller ◽

Olga Will ◽

Sanjay Tiwari ◽

Claus C Gluer ◽

...

Keyword(s):

Soft Tissue ◽

Bone Metabolism ◽

Binding Kinetics ◽

Long Bone

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Hard and soft tissue integration of immediate and delayed implants with a modified coronal macrodesign: Histological, micro-CT and volumetric soft tissue changes from a pre-clinical in vivo study

Journal Of Clinical Periodontology ◽

10.1111/jcpe.12747 ◽

2017 ◽

Vol 44 (8) ◽

pp. 842-853 ◽

Cited By ~ 12

Author(s):

Ignacio Sanz-Martin ◽

Fabio Vignoletti ◽

Javier Nuñez ◽

Maria Permuy ◽

Fernando Muñoz ◽

...

Keyword(s):

Soft Tissue ◽

In Vivo Study ◽

Micro Ct ◽

Tissue Integration ◽

Tissue Changes ◽

Soft Tissue Changes

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A Site-Specific Comparison of the Trabecular Structure in Senescence-Accelerated Mice^|^mdash;Evaluation of Time-Course Changes in Bone Architecture using in Vivo Micro-CT^|^mdash;

Journal of Hard Tissue Biology ◽

10.2485/jhtb.22.171 ◽

2013 ◽

Vol 22 (2) ◽

pp. 171-176 ◽

Cited By ~ 3

Author(s):

Taku Noguchi ◽

Satoru Matsunaga ◽

Hideaki Kinoshita ◽

Masayuki Fukuda ◽

Hideki Saka ◽

...

Keyword(s):

Time Course ◽

Trabecular Structure ◽

Bone Architecture ◽

Micro Ct ◽

Site Specific ◽

Course Changes ◽

A Site ◽

Senescence Accelerated Mice ◽

Specific Comparison

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Ultrastructure of melanocyte-keratinocyte interactions and pigment transfer in vitro

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100084120 ◽

1978 ◽

Vol 36 (2) ◽

pp. 650-651

Author(s):

Raul I. Garcia ◽

Evelyn A. Flynn ◽

George Szabo

Keyword(s):

Direct Injection ◽

Skin Pigmentation ◽

Freeze Fracture ◽

Pigment Granule ◽

Time Lapse ◽

Ultrastructural Level ◽

Lanthanum Tracer ◽

A Cell

Skin pigmentation in mammals involves the interaction of epidermal melanocytes and keratinocytes in the structural and functional unit known as the Epidermal Melanin Unit. Melanocytes(M) synthesize melanin within specialized membrane-bound organelles, the melanosome or pigment granule. These are subsequently transferred by way of M dendrites to keratinocytes(K) by a mechanism still to be clearly defined. Three different, though not necessarily mutually exclusive, mechanisms of melanosome transfer have been proposed: cytophagocytosis by K of M dendrite tips containing melanosomes, direct injection of melanosomes into the K cytoplasm through a cell-to-cell pore or communicating channel formed by localized fusion of M and K cell membranes, release of melanosomes into the extracellular space(ECS) by exocytosis followed by K uptake using conventional phagocytosis. Variability in methods of transfer has been noted both in vivo and in vitro and there is evidence in support of each transfer mechanism. We Have previously studied M-K interactions in vitro using time-lapse cinemicrography and in vivo at the ultrastructural level using lanthanum tracer and freeze-fracture.

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Evaluation of 99mTc-Label led Vitamin K4 as an Agent for Testicular Imaging

Nuklearmedizin ◽

10.1055/s-0038-1629552 ◽

1991 ◽

Vol 30 (01) ◽

pp. 35-39 ◽

Cited By ~ 1

Author(s):

H. S. Durak ◽

M. Kitapgi ◽

B. E. Caner ◽

R. Senekowitsch ◽

M. T. Ercan

Keyword(s):

Soft Tissue ◽

Room Temperature ◽

Normal Subjects ◽

Left Testis ◽

Wide Range ◽

Activity Ratios ◽

The Right ◽

Radioactivity Levels

Vitamin K4 was labelled with 99mTc with an efficiency higher than 97%. The compound was stable up to 24 h at room temperature, and its biodistribution in NMRI mice indicated its in vivo stability. Blood radioactivity levels were high over a wide range. 10% of the injected activity remained in blood after 24 h. Excretion was mostly via kidneys. Only the liver and kidneys concentrated appreciable amounts of radioactivity. Testis/soft tissue ratios were 1.4 and 1.57 at 6 and 24 h, respectively. Testis/blood ratios were lower than 1. In vitro studies with mouse blood indicated that 33.9 ±9.6% of the radioactivity was associated with RBCs; it was washed out almost completely with saline. Protein binding was 28.7 ±6.3% as determined by TCA precipitation. Blood clearance of 99mTc-l<4 in normal subjects showed a slow decrease of radioactivity, reaching a plateau after 16 h at 20% of the injected activity. In scintigraphic images in men the testes could be well visualized. The right/left testis ratio was 1.08 ±0.13. Testis/soft tissue and testis/blood activity ratios were highest at 3 h. These ratios were higher than those obtained with pertechnetate at 20 min post injection.99mTc-l<4 appears to be a promising radiopharmaceutical for the scintigraphic visualization of testes.

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