scholarly journals Disruption of glucocorticoid signaling in chondrocytes delays metaphyseal fracture healing but does not affect normal cartilage and bone development

Bone ◽  
2014 ◽  
Vol 69 ◽  
pp. 12-22 ◽  
Author(s):  
Jinwen Tu ◽  
Holger Henneicke ◽  
Yaqing Zhang ◽  
Shihani Stoner ◽  
Tegan L. Cheng ◽  
...  
Keyword(s):  
Fracture Healing ◽  
Bone Development ◽  
Normal Cartilage ◽  
Glucocorticoid Signaling ◽  
Metaphyseal Fracture ◽  
Metaphyseal Fracture Healing

scholarly journals Animal models for studying metaphyseal bone fracture healing

2020 ◽  
Vol 40 ◽  
pp. 172-188
Author(s):  
M Haffner-Luntzer ◽  
◽  
A Ignatius
Keyword(s):  
Animal Models ◽  
Fracture Healing ◽  
Small Animal ◽  
Osteoporotic Fractures ◽  
Bone Fracture Healing ◽  
Advantages And Disadvantages ◽  
Metaphyseal Fracture ◽  
Small Animal Models ◽  
Metaphyseal Bone ◽  
Metaphyseal Fracture Healing

An estimated 2 million osteoporotic fractures occur annually in the US, resulting in a dramatic reduction in quality of life for affected patients and a high economic burden for society. Osteoporotic fractures are frequently located in metaphyseal bone regions. They are often associated with healing complications, because of the reduced healing capacity of the diseased bone tissue, the poor primary stability of the fracture fixation in the fragile bone, and the high frequency of comorbidities in these patients. Therefore, osteoporotic fractures require optimised treatment strategies to ensure proper bone healing. Preclinical animal models can help understanding of the underlying mechanisms and development of new therapies. However, whereas diaphyseal fracture models are widely available, appropriate animal models for metaphyseal fracture healing are scarce, although essential for translational research. This review covers large and small animal models for metaphyseal fracture healing. General requirements for suitable animal models are presented, as well as advantages and disadvantages of the current models. Furthermore, differences and similarities between metaphyseal and diaphyseal bone fracture healing are discussed. Both large- and small-animal models are available for studying metaphyseal fracture healing, which mainly differ in fracture location and geometry as well as stabilisation techniques. Most common used fracture sites are distal femur and proximal tibia. Each model found in the literature has certain advantages and disadvantages; however, many lack standardisation resulting in a high variability or poor mimicking of the clinical situation. Therefore, further refinement ofanimal models is needed especially to study osteoporotic metaphyseal fracture healing.

Metaphyseal fracture healing in a sheep model of low turnover osteoporosis induced by hypothalamic-pituitary disconnection (HPD)

2013 ◽  
Vol 31 (11) ◽  
pp. 1851-1857 ◽  
Author(s):  
Ronny Bindl ◽  
Ralf Oheim ◽  
Pia Pogoda ◽  
Frank Timo Beil ◽  
Katharina Gruchenberg ◽  
...  
Keyword(s):  
Fracture Healing ◽  
Sheep Model ◽  
Metaphyseal Fracture ◽  
Metaphyseal Fracture Healing

scholarly journals Do Estrogen and Alendronate Improve Metaphyseal Fracture Healing When Applied as Osteoporosis Prophylaxis?

2009 ◽  
Vol 86 (1) ◽  
pp. 23-32 ◽  
Author(s):  
Leila Kolios ◽  
Ann Kristin Hoerster ◽  
Stephan Sehmisch ◽  
Marie Christin Malcherek ◽  
Thomas Rack ◽  
...  
Keyword(s):  
Fracture Healing ◽  
Metaphyseal Fracture ◽  
Osteoporosis Prophylaxis ◽  
Metaphyseal Fracture Healing

scholarly journals Fibrinolysis as a target to enhance osteoporotic fracture healing by vibration therapy in a metaphyseal fracture model

2021 ◽  
Vol 10 (1) ◽  
pp. 41-50
Author(s):  
Ronald Man Yeung Wong ◽  
Victoria Man Huen Choy ◽  
Jie Li ◽  
Tsz Kiu Li ◽  
Yu Ning Chim ◽  
...  
Keyword(s):  
Fracture Healing ◽  
Plasminogen Activator ◽  
Osteoporotic Fracture ◽  
Plasminogen Activator Inhibitor ◽  
Plasminogen Activator Inhibitor 1 ◽  
Non Invasive ◽  
Relevant Animal Model ◽  
Metaphyseal Fracture ◽  
Fibrinolytic Potential ◽  
Metaphyseal Fracture Healing

Aims Fibrinolysis plays a key transition step from haematoma formation to angiogenesis and fracture healing. Low-magnitude high-frequency vibration (LMHFV) is a non-invasive biophysical modality proven to enhance fibrinolytic factors. This study investigates the effect of LMHFV on fibrinolysis in a clinically relevant animal model to accelerate osteoporotic fracture healing. Methods A total of 144 rats were randomized to four groups: sham control; sham and LMHFV; ovariectomized (OVX); and ovariectomized and LMHFV (OVX-VT). Fibrinolytic potential was evaluated by quantifying fibrin, tissue plasminogen activator (tPA), and plasminogen activator inhibitor-1 (PAI-1) along with healing outcomes at three days, one week, two weeks, and six weeks post-fracture. Results All rats achieved healing, and x-ray relative radiopacity for OVX-VT was significantly higher compared to OVX at week 2. Martius Scarlet Blue (MSB) staining revealed a significant decrease of fibrin content in the callus in OVX-VT compared with OVX on day 3 (p = 0.020). Mean tPA from muscle was significantly higher for OVX-VT compared to OVX (p = 0.020) on day 3. Mechanical testing revealed the mean energy to failure was significantly higher for OVX-VT at 37.6 N mm (SD 8.4) and 71.9 N mm (SD 30.7) compared with OVX at 5.76 N mm (SD 7.1) (p = 0.010) and 17.7 N mm (SD 11.5) (p = 0.030) at week 2 and week 6, respectively. Conclusion Metaphyseal fracture healing is enhanced by LMHFV, and one of the important molecular pathways it acts on is fibrinolysis. LMHFV is a promising intervention for osteoporotic metaphyseal fracture healing. The improved mechanical properties, acceleration of fracture healing, and safety justify its role into translation to future clinical studies. Cite this article: Bone Joint Res 2021;10(1):41–50.

Metaphyseal fracture healing in a sheep model of low turnover osteoporosis induced by hypothalamic[ndash]pituitary disconnection

2013 ◽  
Author(s):  
Ronny Bindl ◽  
Ralf Oheim ◽  
Pia Pogoda ◽  
Frank Timo Beil ◽  
Katharina Gruchenberg ◽  
...  
Keyword(s):  
Fracture Healing ◽  
Sheep Model ◽  
Metaphyseal Fracture ◽  
Metaphyseal Fracture Healing

scholarly journals Simulating Metaphyseal Fracture Healing in the Distal Radius

Biomechanics ◽  
2021 ◽  
Vol 1 (1) ◽  
pp. 29-42
Author(s):  
Lucas Engelhardt ◽  
Frank Niemeyer ◽  
Patrik Christen ◽  
Ralph Müller ◽  
Kerstin Stock ◽  
...  
Keyword(s):  
Fracture Healing ◽  
Distal Radius ◽  
Bone Healing ◽  
Numerical Models ◽  
Healing Process ◽  
Patient Specific ◽  
Diaphyseal Fracture ◽  
Metaphyseal Fracture ◽  
Metaphyseal Bone ◽  
Metaphyseal Fracture Healing

Simulating diaphyseal fracture healing via numerical models has been investigated for a long time. It is apparent from in vivo studies that metaphyseal fracture healing should follow similar biomechanical rules although the speed and healing pattern might differ. To investigate this hypothesis, a pre-existing, well-established diaphyseal fracture healing model was extended to study metaphyseal bone healing. Clinical data of distal radius fractures were compared to corresponding geometrically patient-specific fracture healing simulations. The numerical model, was able to predict a realistic fracture healing process in a wide variety of radius geometries. Endochondral and mainly intramembranous ossification was predicted in the fractured area without callus formation. The model, therefore, appears appropriate to study metaphyseal bone healing under differing mechanical conditions and metaphyseal fractures in different bones and fracture types. Nevertheless, the outlined model was conducted in a simplified rotational symmetric case. Further studies may extend the model to a three-dimensional representation to investigate complex fracture shapes. This will help to optimize clinical treatments of radial fractures, medical implant design and foster biomechanical research in metaphyseal fracture healing.

Equol but not Genistein Improves Early Metaphyseal Fracture Healing in Osteoporotic Rats

Planta Medica ◽  
2009 ◽  
Vol 75 (05) ◽  
pp. 459-465 ◽  
Author(s):  
Leila Kolios ◽  
Stephan Sehmisch ◽  
Florian Daub ◽  
Thomas Rack ◽  
Mohammed Tezval ◽  
...  
Keyword(s):  
Fracture Healing ◽  
Metaphyseal Fracture ◽  
Metaphyseal Fracture Healing
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