scholarly journals Mutant p53 gain of function can be at the root of dedifferentiation of human osteosarcoma MG63 cells into 3AB-OS cancer stem cells

Bone ◽  
2014 ◽  
Vol 60 ◽  
pp. 198-212 ◽  
Author(s):  
Riccardo Di Fiore ◽  
Michela Marcatti ◽  
Rosa Drago-Ferrante ◽  
Antonella D'Anneo ◽  
Michela Giuliano ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cancer Stem Cells ◽  
Mutant P53 ◽  
Gain Of Function ◽  
Human Osteosarcoma ◽  
Mg63 Cells

scholarly journals Mutant p53 gain of function underlies high expression levels of colorectal cancer stem cells markers

Oncogene ◽  
2018 ◽  
Vol 37 (12) ◽  
pp. 1669-1684 ◽  
Author(s):  
Hilla Solomon ◽  
Nathan Dinowitz ◽  
Ioannis S. Pateras ◽  
Tomer Cooks ◽  
Yoav Shetzer ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Stem Cells ◽  
Cancer Stem Cells ◽  
High Expression ◽  
Mutant P53 ◽  
Gain Of Function ◽  
Expression Levels ◽  
Colorectal Cancer Stem Cells

Identification and expansion of human osteosarcoma-cancer-stem cells by long-term 3-aminobenzamide treatment

2009 ◽  
Vol 219 (2) ◽  
pp. 301-313 ◽  
Author(s):  
Riccardo Di Fiore ◽  
Andrea Santulli ◽  
Rosa Drago Ferrante ◽  
Michela Giuliano ◽  
Anna De Blasio ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cancer Stem Cells ◽  
Human Osteosarcoma

scholarly journals The paradigm of mutant p53-expressing cancer stem cells and drug resistance

2014 ◽  
Vol 35 (6) ◽  
pp. 1196-1208 ◽  
Author(s):  
Yoav Shetzer ◽  
Hilla Solomon ◽  
Gabriela Koifman ◽  
Alina Molchadsky ◽  
Stav Horesh ◽  
...  
Keyword(s):  
Stem Cells ◽  
Drug Resistance ◽  
Cancer Stem Cells ◽  
Mutant P53

scholarly journals MicroRNA-29b-1 impairs in vitro cell proliferation, self-renewal and chemoresistance of human osteosarcoma 3AB-OS cancer stem cells

2014 ◽  
Vol 45 (5) ◽  
pp. 2013-2023 ◽  
Author(s):  
RICCARDO DI FIORE ◽  
ROSA DRAGO-FERRANTE ◽  
FRANCESCA PENTIMALLI ◽  
DOMENICO DI MARZO ◽  
IRIS MARIA FORTE ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cell Proliferation ◽  
Cancer Stem Cells ◽  
Self Renewal ◽  
Human Osteosarcoma

scholarly journals Isolation, Culture and Characterization of Cancer Stem Cells from Primary Osteosarcoma

2018 ◽  
Vol 5 (1) ◽  
pp. 1-13 ◽  
Author(s):  
Achmad Fauzi Kamal ◽  
Deded Yudha Pranatha ◽  
Waluyo Sugito ◽  
Faisal Rahman ◽  
Eka Susanto ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Cancer Stem Cells ◽  
Embryonic Stem ◽  
Osteosarcoma Cells ◽  
Rt Pcr ◽  
Alizarin Red ◽  
Human Osteosarcoma ◽  
Expression Of Genes ◽  
Assay Technique

Background:Osteosarcoma cancer stem cells (CSCs) are defined as a subpopulation of osteosarcoma cells, which have the ability of self-renewal, proliferation and differentiation. This study aimed to identify CSCs from human osteosarcomain vitro.Methods:Osteosarcoma CSCs were isolated and cultured with sphere-forming assay technique on an ultra-low well attachment surface plate. After sarcosphere colonies were formed, we conducted reverse transcriptase-polymerase chain reaction (RT-PCR) to detect the expression of genes of embryonic stem cells such asNANOG, Oct3/4, STAT3 and gene of MSC CD133. Immunofluorescence analysis (IFA) of alkaline phosphatase (ALP), osteocalcin, and CD 133 was also performed to see the expression of osteosarcoma CSC surface protein with immuno-enzymatic staining principle. We also performed alizarin red staining to evaluate calcification in osteosarcoma CSCs.Results:The culture sphere-of the osteosarcoma cells showed three dimension round shaped colonies (sarcospheres) in slightly hypoxicand serum free condition which was not attached to the substrate with tight density. RT-PCR demonstrated that sarcospheres expressed genes which encodeNANOG, Oct3/4 STAT 3, but not for CD 133. IFA showed positive protein expression of ALP, osteocalcin and CD 133 which was moderate, strong, and weak positive respectively. Sarcospheres also had a positive reaction toward alizarin red staining.Conclusion:Osteosarcoma CSCs could be isolated from human osteosarcoma by sphere-forming assay technique and characterized by the expression of genes of embryonic stem cells,such asNANOG, Oct3/4, STAT3 and IFA of ALP, osteocalcin, and CD 133.

scholarly journals Cancer Stemness: p53 at the Wheel

2021 ◽  
Vol 10 ◽  
Author(s):  
Dishari Ghatak ◽  
Damayanti Das Ghosh ◽  
Susanta Roychoudhury
Keyword(s):  
Stem Cells ◽  
Cancer Cells ◽  
Adult Stem Cells ◽  
Critical Role ◽  
Mutant P53 ◽  
Gain Of Function ◽  
Cancer Stemness ◽  
Self Renewal ◽  
Mesenchymal Transition

The tumor suppressor p53 maintains an equilibrium between self-renewal and differentiation to sustain a limited repertoire of stem cells for proper development and maintenance of tissue homeostasis. Inactivation of p53 disrupts this balance and promotes pluripotency and somatic cell reprogramming. A few reports in recent years have indicated that prevalent TP53 oncogenic gain-of-function (GOF) mutations further boosts the stemness properties of cancer cells. In this review, we discuss the role of wild type p53 in regulating pluripotency of normal stem cells and various mechanisms that control the balance between self-renewal and differentiation in embryonic and adult stem cells. We also highlight how inactivating and GOF mutations in p53 stimulate stemness in cancer cells. Further, we have explored the various mechanisms of mutant p53-driven cancer stemness, particularly emphasizing on the non-coding RNA mediated epigenetic regulation. We have also analyzed the association of cancer stemness with other crucial gain-of-function properties of mutant p53 such as epithelial to mesenchymal transition phenotypes and chemoresistance to understand how activation of one affects the other. Given the critical role of cancer stem-like cells in tumor maintenance, cancer progression, and therapy resistance of mutant p53 tumors, targeting them might improve therapeutic efficacy in human cancers with TP53 mutations.

scholarly journals BMP-2 inhibits the tumorigenicity of cancer stem cells in human osteosarcoma OS99-1 cell line

2011 ◽  
Vol 11 (5) ◽  
pp. 457-463 ◽  
Author(s):  
Lin Wang ◽  
Paul Park ◽  
Huina Zhang ◽  
Frank La Marca ◽  
Amy Claeson ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cancer Stem Cells ◽  
Cell Line ◽  
Human Osteosarcoma

scholarly journals Mutant p53 oncogenic functions in cancer stem cells are regulated by WIP through YAP/TAZ

Oncogene ◽  
2017 ◽  
Vol 36 (25) ◽  
pp. 3515-3527 ◽  
Author(s):  
M Escoll ◽  
R Gargini ◽  
A Cuadrado ◽  
I M Anton ◽  
F Wandosell
Keyword(s):  
Stem Cells ◽  
Cancer Stem Cells ◽  
Mutant P53
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