Repair of critical-size mouse cranial and long bone defects using human adult mesenchymal stromal cells expressing integrin alpha 5

Bone ◽  
2011 ◽  
Vol 48 ◽  
pp. S170
Author(s):  
S. Srouji ◽  
O. Fromigué ◽  
P. Vaudin ◽  
D. Ben-David ◽  
E. Livne ◽  
...  
Keyword(s):  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Critical Size ◽  
Bone Defects ◽  
Long Bone ◽  
Human Adult

Lentiviral-Mediated Integrin α5 Expression in Human Adult Mesenchymal Stromal Cells Promotes Bone Repair in Mouse Cranial and Long-Bone Defects

2012 ◽  
Vol 23 (2) ◽  
pp. 167-172 ◽  
Author(s):  
Samer Srouji ◽  
Dror Ben-David ◽  
Olivia Fromigué ◽  
Pascal Vaudin ◽  
Gisela Kuhn ◽  
...  
Keyword(s):  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Bone Repair ◽  
Bone Defects ◽  
Long Bone ◽  
Human Adult ◽  
Integrin Α5

Designing microtissue bioassemblies for skeletal regeneration: Healing critical size long bone defects

Cytotherapy ◽  
2018 ◽  
Vol 20 (5) ◽  
pp. S14
Author(s):  
G. Nilsson Hall ◽  
L. Mendes ◽  
L. Geris ◽  
I. Papantoniou ◽  
F. Luyten
Keyword(s):  
Critical Size ◽  
Bone Defects ◽  
Long Bone ◽  
Skeletal Regeneration

scholarly journals Limited Acquisition of Chromosomal Aberrations in Human Adult Mesenchymal Stromal Cells

2012 ◽  
Vol 10 (1) ◽  
pp. 9-10 ◽  
Author(s):  
Luc Sensebé ◽  
Karin Tarte ◽  
Jacques Galipeau ◽  
Mauro Krampera ◽  
Ivan Martin ◽  
...  
Keyword(s):  
Mesenchymal Stromal Cells ◽  
Chromosomal Aberrations ◽  
Stromal Cells ◽  
Human Adult

Characterization of Platelet Lysate Cultured Mesenchymal Stromal Cells and Their Potential Use in Tissue-Engineered Osteogenic Devices for the Treatment of Bone Defects

2010 ◽  
Vol 16 (2) ◽  
pp. 201-214 ◽  
Author(s):  
Agnese Salvadè ◽  
Pamela Della Mina ◽  
Diego Gaddi ◽  
Francesca Gatto ◽  
Antonello Villa ◽  
...  
Keyword(s):  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Bone Defects ◽  
Platelet Lysate ◽  
Potential Use

scholarly journals The Role of Pannexin3-Modified Human Dental Pulp-Derived Mesenchymal Stromal Cells in Repairing Rat Cranial Critical-Sized Bone Defects

2017 ◽  
Vol 44 (6) ◽  
pp. 2174-2188 ◽  
Author(s):  
Fangfang Song ◽  
Hualing Sun ◽  
Liyuan Huang ◽  
Dongjie Fu ◽  
Cui Huang
Keyword(s):  
Osteogenic Differentiation ◽  
Signaling Pathway ◽  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Dental Pulp ◽  
Underlying Mechanism ◽  
Bone Defects ◽  
Dependent Manner ◽  
Alizarin Red ◽  
Human Dental Pulp

Background/Aims: Human dental pulp-derived mesenchymal stromal cells (hDPSCs) are promising seed cells for tissue engineering due to their easy accessibility and multi-lineage differentiation. Pannexin3 (Panx3) plays crucial roles during bone development and differentiation. The aim of the present study was to investigate the effect of Panx3 on osteogenesis of hDPSCs and the underlying mechanism. Methods: Utilizing qRT-PCR, Western blot, and immunohistochemistry, we explored the change of Panx3 during osteogenic differentiation of hDPSCs. Next, hDPSCs with loss (Panx3 knockdown) and gain (Panx3 overexpression) of Panx3 function were developed to investigate the effects of Panx3 on osteogenic differentiation of hDPSC and the underlying mechanism. Finally, a commercial β-TCP scaffold carrying Panx3-modified hDPSCs was utilized to evaluate bone defect repair. Results: Panx3 was upregulated during osteogenic differentiation in a time-dependent manner. Panx3 overexpression promoted osteogenic differentiation of hDPSCs, whereas depletion of Panx3 resulted in a decline of differentiation, evidenced by upregulated expression of mineralization-related markers, increased alkaline phosphatase (ALP) activity, and enhanced ALP and Alizarin red staining. Panx3 was found to interact with the Wnt/β-catenin signaling pathway, forming a negative feedback loop. However, Wnt/β-catenin did not contribute to enhancement of osteogenic differentiation as observed in Panx3 overexpression. Moreover, Panx3 promoted osteogenic differentiation of hDPSCs via increasing ERK signaling pathway. Micro-CT and histological staining results showed that Panx3-modified hDPSCs significantly improved ossification of critical-sized bone defects. Conclusion: These findings suggest that Panx3 is a crucial modulator of hDPSCs differentiation.

scholarly journals Efficacy and Safety of Human Mesenchymal Stromal Cells in Healing of Critical-Size Bone Defects in Immunodeficient Rats

2017 ◽  
pp. 113-123 ◽  
Author(s):  
R. PYTLÍK ◽  
C. RENTSCH ◽  
T. SOUKUP ◽  
L. NOVOTNÝ ◽  
B. RENTSCH ◽  
...  
Keyword(s):  
Healthy Volunteers ◽  
Stromal Cells ◽  
Critical Size ◽  
Human Mesenchymal Stem Cells ◽  
Bone Defects ◽  
Efficacy And Safety ◽  
X Ray ◽  
Residual Defect ◽  
Healthy Donors ◽  
Entire Duration

To evaluate the preclinical efficacy and safety of human mesenchymal stem cells (hMSC) rapidly expanded in growth medium for clinical use with human serum and recombinant growth factors, we conducted a controlled, randomized trial of plasma clots with hMSC vs. plasma clots only in critical segmental femoral defects in rnu/rnu immunodeficient rats. X-ray, microCT and histomorphometrical evaluation were performed at 8 and 16 weeks. MSC were obtained from healthy volunteers and patients with lymphoid malignancy. Human MSC survived in the defect for the entire duration of the trial. MSC from healthy volunteers, in contrast to hMSC from cancer patients, significantly improved bone healing at 8, but not 16 weeks. However, at 16 weeks, hMSC significantly improved vasculogenesis in residual defect. We conclude that hMSC from healthy donors significantly contributed to the healing of bone defects at 8 weeks and to the vascularisation of residual connective tissue for up to 16 weeks. We found the administration of hMSC to be safe, as no adverse reaction to human cells at the site of implantation and no evidence of migration of hMSC to distant organs was detected.

scholarly journals Human pluripotent stem cell-derived cartilaginous organoids promote scaffold-free healing of critical size long bone defects

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Wai Long Tam ◽  
Luís Freitas Mendes ◽  
Xike Chen ◽  
Raphaëlle Lesage ◽  
Inge Van Hoven ◽  
...  
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Pluripotent Stem Cells ◽  
Pluripotent Stem Cell ◽  
Critical Size ◽  
Bone Defects ◽  
Long Bone ◽  
Tissue Formation ◽  
Induced Pluripotent

Abstract Background Bones have a remarkable capacity to heal upon fracture. Yet, in large defects or compromised conditions healing processes become impaired, resulting in delayed or non-union. Current therapeutic approaches often utilize autologous or allogeneic bone grafts for bone augmentation. However, limited availability of these tissues and lack of predictive biological response result in limitations for clinical demands. Tissue engineering using viable cell-based implants is a strategic approach to address these unmet medical needs. Methods Herein, the in vitro and in vivo cartilage and bone tissue formation potencies of human pluripotent stem cells were investigated. The induced pluripotent stem cells were specified towards the mesodermal lineage and differentiated towards chondrocytes, which subsequently self-assembled into cartilaginous organoids. The tissue formation capacity of these organoids was then challenged in an ectopic and orthotopic bone formation model. Results The derived chondrocytes expressed similar levels of collagen type II as primary human articular chondrocytes and produced stable cartilage when implanted ectopically in vivo. Upon targeted promotion towards hypertrophy and priming with a proinflammatory mediator, the organoids mediated successful bridging of critical size long bone defects in immunocompromised mice. Conclusions These results highlight the promise of induced pluripotent stem cell technology for the creation of functional cartilage tissue intermediates that can be explored for novel bone healing strategies.

scholarly journals Utilizing Autologous Multipotent Mesenchymal Stromal Cells andβ-Tricalcium Phosphate Scaffold in Human Bone Defects: A Prospective, Controlled Feasibility Trial

2016 ◽  
Vol 2016 ◽  
pp. 1-12 ◽  
Author(s):  
Pavel Šponer ◽  
Stanislav Filip ◽  
Tomáš Kučera ◽  
Jindra Brtková ◽  
Karel Urban ◽  
...  
Keyword(s):  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Tricalcium Phosphate ◽  
Bone Defects ◽  
Feasibility Trial ◽  
Harris Hip Score ◽  
Controlled Study ◽  
Control Group ◽  
Trial Group ◽  
Significant Difference

The purpose of this prospective controlled study was to compare healing quality following the implantation of ultraporousβ-tricalcium phosphate, containing either expanded autologous mesenchymal stromal cells (trial group, 9 patients) orβ-tricalcium phosphate alone (control group, 9 patients), into femoral defects during revision total hip arthroplasty. Both groups were assessed using the Harris Hip Score, radiography, and DEXA scanning at 6 weeks and 3, 6, and 12 months postoperatively. A significant difference in the bone defect healing was observed between both groups of patients (P<0.05). In the trial group, trabecular remodeling was found in all nine patients and in the control group, in 1 patient only. Whereas, over the 12-month follow-up period, no significant difference was observed between both groups of patients in terms of the resorption ofβ-tricalcium phosphate, the significant differences were documented in the presence of radiolucency and bone trabeculation through the defect (P<0.05). Using autologous mesenchymal stromal cells combined with aβ-tricalcium phosphate scaffold is a feasible, safe, and effective approach for management of bone defects with compromised microenvironment. The clinical trial was registered at the EU Clinical Trials Register before patient recruitment has begun (EudraCT number2012-005599-33).

scholarly journals Technology for Repairing Osteomyelitic Bone Defects Using Autologous Mesenchymal Stromal Cells on a Collagen Matrix in Experiment

2021 ◽  
Vol 13 (1) ◽  
pp. 42
Author(s):  
V.N. Mitrofanov ◽  
O.P. Zhivtsov ◽  
N.Yu. Orlinskaya ◽  
D.V. Davydenko ◽  
I.N. Charykova ◽  
...  
Keyword(s):  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Collagen Matrix ◽  
Bone Defects
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