Protective effect of alendronate on articular cartilage and subchondral bone in osteoarthritic rabbit model

Bone ◽  
2010 ◽  
Vol 47 ◽  
pp. S394
Author(s):  
Liu Zhang ◽  
Tan Cheng
Keyword(s):  
Articular Cartilage ◽  
Protective Effect ◽  
Subchondral Bone ◽  
Rabbit Model

High Fibular Osteotomy Ameliorates Medial Compartment Knee Osteoarthritis in a Rabbit Model

2021 ◽  
Author(s):  
Feihua Yan ◽  
Xujun Zhao ◽  
Shisheng Duan ◽  
Aini Maimaiti ◽  
Yong Qi ◽  
...  
Keyword(s):  
Articular Cartilage ◽  
Knee Osteoarthritis ◽  
Bone Remodeling ◽  
Western Blotting ◽  
Subchondral Bone ◽  
Rabbit Model ◽  
Medial Compartment ◽  
Enzyme Linked Immunosorbent Assay ◽  
Fibular Osteotomy ◽  
Tnf Α

Abstract Purpose Knee osteoarthritis (KOA) is a common and severe disease characterized by articular cartilage degeneration, subchondral bone remodeling and inflammation. This study aimed to investigate the therapeutic effects of high fibular osteotomy (HFO) in a KOA rabbit model and to examine the molecular mechanisms involved in medial compartment KOA protective effects.Methods A rabbit model of destabilization of the medial meniscus was used to induce post-traumatic KOA. The effectiveness of HFO on protection against KOA was tested. Hematoxylin and eosin staining, Safranin O/Fast green staining and micro-CT analysis were performed to evaluate structural and morphological changes. The expression of metalloproteinase (MMP)-1, MMP-3, MMP-13, collagen type II (Col2), a disintegrin and metalloproteinase domain with thrombospondin motifs (ADAMTS)-5, aggrecan, interleukin (IL)-1β, IL-6 and tumor necrosis factor (TNF)-α was assessed by real time PCR, western blotting and enzyme-linked immunosorbent assay. Additionally, western blotting was performed to test the expression of NFκB p65, phospho-IκBα and IκBα. Results HFO delayed the progression of articular cartilage damage and suppressed subchondral bone remodeling. HFO also decreased MMP-1, MMP-3, MMP-13 and ADAMTS-5 expression, and increased Col2 and aggrecan expression. In parallel, HFO attenuated the expression of IL-1β, IL-6 and TNF-α. Furthermore, the molecular mechanism underlying the protective effect of HFO in medial compartment KOA was related to the NFκB signaling pathway. Conclusion HFO may be a novel therapeutic approach to treating medial compartment KOA.

scholarly journals 093 EFFECTS OF ALENDRONATE ON ARTICULAR CARTILAGE AND SUBCHONDRAL BONE CHANGES IN A RABBIT MODEL OF OSTEOARTHRITIS

2009 ◽  
Vol 17 ◽  
pp. S58
Author(s):  
T. Shirai ◽  
M. Kobayashi ◽  
K. Nishitani ◽  
T. Satake ◽  
H. Kuroki ◽  
...  
Keyword(s):  
Articular Cartilage ◽  
Subchondral Bone ◽  
Rabbit Model ◽  
Bone Changes

scholarly journals Genetic Deletion of Interleukin-15 Is Not Associated with Major Structural Changes Following Experimental Post-Traumatic Knee Osteoarthritis in Rats

2021 ◽  
Vol 11 (15) ◽  
pp. 7118
Author(s):  
Ermina Hadzic ◽  
Garth Blackler ◽  
Holly Dupuis ◽  
Stephen James Renaud ◽  
Christopher Thomas Appleton ◽  
...  
Keyword(s):  
Articular Cartilage ◽  
Subchondral Bone ◽  
Structural Changes ◽  
Cartilage Damage ◽  
Wild Type ◽  
Significant Difference ◽  
Post Traumatic ◽  
Interleukin 15 ◽  
Joint Trauma ◽  
Surgically Induced

Post-traumatic osteoarthritis (PTOA) is a degenerative joint disease, leading to articular cartilage breakdown, osteophyte formation, and synovitis, caused by an initial joint trauma. Pro-inflammatory cytokines increase catabolic activity and may perpetuate inflammation following joint trauma. Interleukin-15 (IL-15), a pro-inflammatory cytokine, is increased in OA patients, although its roles in PTOA pathophysiology are not well characterized. Here, we utilized Il15 deficient rats to examine the role of IL-15 in PTOA pathogenesis in an injury-induced model. OA was surgically induced in Il15 deficient Holtzman Sprague-Dawley rats and control wild-type rats to compare PTOA progression. Semi-quantitative scoring of the articular cartilage, subchondral bone, osteophyte size, and synovium was performed by two blinded observers. There was no significant difference between Il15 deficient rats and wild-type rats following PTOA-induction across articular cartilage damage, subchondral bone damage, and osteophyte scoring. Similarly, synovitis scoring across six parameters found no significant difference between genetic variants. Overall, IL-15 does not appear to play a key role in the development of structural changes in this surgically-induced rat model of PTOA.

scholarly journals Early patellofemoral articular cartilage degeneration in a rat model of patellar instability is associated with activation of the NF-κB signaling pathway

2021 ◽  
Vol 22 (1) ◽  
Author(s):  
Wei Lin ◽  
Huijun Kang ◽  
Yike Dai ◽  
Yingzhen Niu ◽  
Guangmin Yang ◽  
...  
Keyword(s):  
Articular Cartilage ◽  
Signaling Pathway ◽  
Subchondral Bone ◽  
Patellofemoral Joint ◽  
Patellar Instability ◽  
Cartilage Degeneration ◽  
Control Group ◽  
And Control ◽  
Articular Cartilage Degeneration ◽  
And Cartilage

Abstract Background Patellar instability (PI) often increases the possibility of lateral patellar dislocation and early osteoarthritis. The molecular mechanism of early articular cartilage degeneration during patellofemoral osteoarthritis (PFOA) still requires further investigation. However, it is known that the NF-κB signaling pathway plays an important role in articular cartilage degeneration. The aim of this study was to investigate the relationship between the NF-κB signaling pathway and patellofemoral joint cartilage degeneration. Methods We established a rat model of PI-induced PFOA. Female 4-week-old Sprague-Dawley rats (n = 120) were randomly divided into two groups: the PI (n = 60) and control group (n = 60). The distal femurs of the PI and control group were isolated and compared 4, 8, and 12 weeks after surgery. The morphological structure of the trochlear cartilage and subchondral bone were evaluated by micro-computed tomography and histology. The expression of NF-κB, matrix metalloproteinase (MMP)-13, collagen X, and TNF-ɑ were evaluated by immunohistochemistry and quantitative polymerase chain reaction. Results In the PI group, subchondral bone loss and cartilage degeneration were found 4 weeks after surgery. Compared with the control group, the protein and mRNA expression of NF-κB and TNF-ɑ were significantly increased 4, 8, and 12 weeks after surgery in the PI group. In addition, the markers of cartilage degeneration MMP-13 and collagen X were more highly expressed in the PI group compared with the control group at different time points after surgery. Conclusions This study has demonstrated that early patellofemoral joint cartilage degeneration can be caused by PI in growing rats, accompanied by significant subchondral bone loss and cartilage degeneration. In addition, the degeneration of articular cartilage may be associated with the activation of the NF-κB signaling pathway and can deteriorate with time as a result of PI.

scholarly journals P150 The protective effect of OP-1 on articular cartilage in the development of osteoarthritis

2007 ◽  
Vol 15 ◽  
pp. B125
Author(s):  
R.D. Coutts ◽  
N. Badlani ◽  
A. Inoue ◽  
R. Healey ◽  
D. Amiel
Keyword(s):  
Articular Cartilage ◽  
Protective Effect

Histological Features of Osteoarthritis in a State of Decompensation

2021 ◽  
Vol 53 ◽  
pp. 51-58
Author(s):  
Timur B. Minasov ◽  
Ekaterina R. Yakupova ◽  
Dilmurod Ruziboev ◽  
Ruslan M. Vakhitov-Kovalevich ◽  
Ruslan F. Khairutdinov ◽  
...  
Keyword(s):  
Articular Cartilage ◽  
Knee Joint ◽  
Blood Vessels ◽  
Subchondral Bone ◽  
Musculoskeletal System ◽  
Hyaline Cartilage ◽  
Material Selection ◽  
Central Zone ◽  
Cartilage Tissue ◽  
Social Significance

Degenerative pathology of the musculoskeletal system is one of the main reasons for decreased mobility in patients of the older age group. Increasing the life expectancy leads to predominance non-epidemic pathology in all developed countries. Therefore, degenerative diseases of musculoskeletal system have not only medical significance but also social significance. Objective is studying the morphological features of synovial environment of the decompensated osteoarthritic (OA) knee joint. Structural features of subchondral bone, hyaline cartilage of the femur and tibia, the articular capsule, menisci and ligamentous apparatus of the knee joint were studied in 64 patients who underwent total knee arthroplasty at the Department of Traumatology and Orthopedics Bashkirian State Medical University in the period from 2015 to 2020. Material selection, preparation of histological samples, staining with hematoxylin-eosin, microscopy was performed. Adaptive signs of articular cartilage of the femoral condyles manifest in the form of cartilage tissue rearrangement, which are most pronounced in the central zone of the cartilage. At the same time, the phenomena of decompensation and significant areas of destruction are noted. Also, the subchondral bone was replaced with connective tissue with subsequent sclerosis. This sclerosis subsequently led to the decompensation of structures of the hyaline cartilage in the deep and middle zones. Destructive and dystrophic processes were noted in the knee joint menisci. Articular cartilage was replaced with granulation tissue with subsequent invasion of blood vessels. Cruciate ligaments in patients with OA show signs of adaptation due to expansion of endothenonium layers between bundles of collagen fibers and an increase in the diameter of blood vessels.

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