High bilirubin reduces cell survival and differentiation of primary human osteoblasts. Is this effect applicable to the serum of chronic cholestatic patients?

Bone ◽  
2010 ◽  
Vol 47 ◽  
pp. S131 ◽  
Author(s):  
S. Ruiz-Gaspà ◽  
A. Martinez-Ferrer ◽  
A. Enjuanes ◽  
P. Peris ◽  
M.J. Martinez de Osaba ◽  
...  
Keyword(s):  
Cell Survival ◽  
Human Osteoblasts ◽  
Primary Human Osteoblasts ◽  
High Bilirubin

535 HIGH BILIRUBIN REDUCES CELL SURVIVAL AND DIFFERENTIATION OF PRIMARY HUMAN OSTEOBLASTS. IS THIS EFFECT APPLICABLE TO THE SERUM OF CHRONIC CHOLESTATIC PATIENTS?

2010 ◽  
Vol 52 ◽  
pp. S214-S215 ◽  
Author(s):  
S. Ruiz-Gaspà ◽  
A. Martinez-Ferrer ◽  
A. Enjuanes ◽  
P. Peris ◽  
M.J. Mertinez de Osaba ◽  
...  
Keyword(s):  
Cell Survival ◽  
Human Osteoblasts ◽  
Primary Human Osteoblasts ◽  
High Bilirubin

Lithocholic acid decreases cell survival and downregulates the effects of vitamin d3 on primary human osteoblasts

Bone ◽  
2009 ◽  
Vol 44 ◽  
pp. S319-S320
Author(s):  
S. Ruiz-Gaspà ◽  
A. Enjuanes ◽  
P. Peris ◽  
A. Martinez-Ferrer ◽  
M. Martínez de Osaba ◽  
...  
Keyword(s):  
Cell Survival ◽  
Vitamin D3 ◽  
Lithocholic Acid ◽  
Human Osteoblasts ◽  
Primary Human Osteoblasts

scholarly journals Adhesion Behaviour of Primary Human Osteoblasts and Fibroblasts on Polyether Ether Ketone Compared with Titanium under In Vitro Lipopolysaccharide Incubation

Materials ◽  
2019 ◽  
Vol 12 (17) ◽  
pp. 2739 ◽  
Author(s):  
Korbinian Benz ◽  
Andreas Schöbel ◽  
Marisa Dietz ◽  
Peter Maurer ◽  
Jochen Jackowski
Keyword(s):  
Gene Expression ◽  
Protein Level ◽  
Binding Protein ◽  
Sem Images ◽  
Human Osteoblasts ◽  
Polyether Ether Ketone ◽  
Primary Human Osteoblasts ◽  
Titanium Surfaces ◽  
Ether Ketone

The aim of this in vitro pilot study was to analyse the adhesion behaviour of human osteoblasts and fibroblasts on polyether ether ketone (PEEK) when compared with titanium surfaces in an inflammatory environment under lipopolysaccharide (LPS) incubation. Scanning electron microscopy (SEM) images of primary human osteoblasts/fibroblasts on titanium/PEEK samples were created. The gene expression of the LPS-binding protein (LBP) and the LPS receptor (toll-like receptor 4; TLR4) was measured by real-time polymerase chain reaction (PCR). Immunocytochemistry was used to obtain evidence for the distribution of LBP/TLR4 at the protein level of the extra-cellular-matrix-binding protein vinculin and the actin cytoskeleton. SEM images revealed that the osteoblasts and fibroblasts on the PEEK surfaces had adhesion characteristics comparable to those of titanium. The osteoblasts contracted under LPS incubation and a significantly increased LBP gene expression were detected. This was discernible at the protein level on all the materials. Whereas no increase of TLR4 was detected with regard to mRNA concentrations, a considerable increase in the antibody reaction was detected on all the materials. As is the case with titanium, the colonisation of human osteoblasts and fibroblasts on PEEK samples is possible under pro-inflammatory environmental conditions and the cellular inflammation behaviour towards PEEK is lower than that of titanium.

In vitro Growth Pattern of Primary Human Osteoblasts on Calcium Phosphate- and Polymethylmethacrylate-Based Bone Cement

2017 ◽  
Vol 58 (5-6) ◽  
pp. 216-226
Author(s):  
Johannes Schauwecker ◽  
Mark Bock ◽  
Florian Pohlig ◽  
Heinz Mühlhofer ◽  
Jutta Tübel ◽  
...  
Keyword(s):  
Cell Differentiation ◽  
Calcium Phosphate ◽  
Cell Viability ◽  
Proliferation Rate ◽  
Control Analysis ◽  
Implant Loosening ◽  
Human Osteoblasts ◽  
Apoptosis Rate ◽  
Primary Human Osteoblasts

Background/Purpose: Polymethylmethacrylate (PMMA) and calcium phosphate (Ca-P) cements are widely used for arthroplasty surgery and augmentation of bone defects. However, aseptic implant loosening in absence of wear-induced osteolysis indicates an unfavourable interaction between the cement surface and human osteoblasts. Our underlying hypothesis is that cement surfaces directly modify cell viability, proliferation rate, and cell differentiation. Methods: To test this hypothesis, we examined primary human osteoblasts harvested from six individuals. These cells were pooled and subsequently seeded directly on cement pellets prepared from Palacos® R, Palacos® R+G, and Norian® Drillable cements. After incubation for 24 and 72 h, cell viability, proliferation rate, apoptosis rate, and cell differentiation were analysed. Results: Upon cultivation of human osteoblasts on cement surfaces, we observed a significantly reduced cell viability and DNA content compared to the control. Analysis of the apoptosis rate revealed an increase for cells on Palacos R and Norian Drillable, but a significant decrease on Palacos R+G compared to the control. Regarding osteogenic differentiation, significantly lower values of alkaline phosphatase enzyme activity were identified for all cement surfaces after 24 and 72 h compared to cultivation on tissue culture plastic, serving as control. Conclusions: In summary, these data suggest a limited biocompatibility of both PMMA and Ca-P cements, necessitating further research to reduce unfavourable cell-cement interactions and consequently extend implant survival.

The enhancing effect of a laser photochemotherapy with cisplatin or zolendronic acid in primary human osteoblasts and osteosarcoma cells in vitro

2016 ◽  
Vol 45 (10) ◽  
pp. 803-809 ◽  
Author(s):  
Paul Günther Baptist Heymann ◽  
Thomas Ziebart ◽  
Peer Wolfgang Kämmerer ◽  
Robert Mandic ◽  
Akram Saydali ◽  
...  
Keyword(s):  
Osteosarcoma Cells ◽  
Human Osteoblasts ◽  
Enhancing Effect ◽  
Zolendronic Acid ◽  
Primary Human Osteoblasts

Differences in morphogenesis of 3D cultured primary human osteoblasts under static and microfluidic growth conditions

Biomaterials ◽  
2014 ◽  
Vol 35 (10) ◽  
pp. 3208-3219 ◽  
Author(s):  
Brigitte Altmann ◽  
Anne Löchner ◽  
Michael Swain ◽  
Ralf-Joachim Kohal ◽  
Stefan Giselbrecht ◽  
...  
Keyword(s):  
Growth Conditions ◽  
Human Osteoblasts ◽  
Primary Human Osteoblasts

scholarly journals Regulation of CYP27B1 mRNA Expression in Primary Human Osteoblasts

2016 ◽  
Vol 99 (2) ◽  
pp. 164-173 ◽  
Author(s):  
K. van der Meijden ◽  
H. W. van Essen ◽  
F. W. Bloemers ◽  
E. A. J. M. Schulten ◽  
P. Lips ◽  
...  
Keyword(s):  
Mrna Expression ◽  
Human Osteoblasts ◽  
Primary Human Osteoblasts
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