Gene expression of MC3T3-E1 osteoblastic cells cultured on titanium surface and zirconia surface

Bone ◽  
2010 ◽  
Vol 47 ◽  
pp. S125
Author(s):  
H. Lee ◽  
S. Ko
Keyword(s):  
Gene Expression ◽  
Titanium Surface ◽  
Osteoblastic Cells ◽  
Cells Cultured

Gene-Expression of MC3T3-E1 Osteoblast Cells Cultured on Anodized Titanium Surface and Machined Titanium Surface by Using cDNA Microarray

2007 ◽  
Vol 361-363 ◽  
pp. 1091-1094
Author(s):  
S.H. Park ◽  
J.M. Park ◽  
H.S. Lee ◽  
K.S. Min ◽  
A.R. Pae ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cdna Microarray ◽  
Titanium Surface ◽  
Control Groups ◽  
Machined Surface ◽  
Implant Surface ◽  
Titanium Surfaces ◽  
Porous Oxide ◽  
Anodized Titanium ◽  
Cells Cultured

One of the techniques for modifying Ti implant surface is an anodization. This method is an electrochemical one that forms rough, thick, and porous oxide films. To compare the gene expression from the cells cultured on the different implant surface, cDNA microarray have been performed. MC3T3-E1 cells were cultured on titanium disks. The control groups were cultured on the machined surfaces(MS). The experimental groups cultured on the anodized titanium surfaces(AS). Microarray assay 48 hours after culturing the cells on the machined surface and anodized surface revealed that osteoinductive molecules appeared more prominent on the anodized surface, whereas the adhesion molecules on the biomaterial were higher on the machined surface than anodized surface, which will affect the phenotype of the plated cells depending on the surface morphology.

Reparação óssea no implante dental

2020 ◽  
Vol 12 (45) ◽  
pp. 63-66
Author(s):  
Halim Nagem Filho ◽  
Reinaldo Francisco Maia ◽  
Reinaldo Missaka ◽  
Nasser Hussein Fares
Keyword(s):  
Gene Expression ◽  
Titanium Surface ◽  
Close Contact ◽  
The Other ◽  
Main Function ◽  
Indirect Interaction ◽  
M2 Macrophages ◽  
Activated Macrophages ◽  
M1 Macrophages ◽  
High Production

The osseointegration is the stable and functional union between the bone and a titanium surface. A new bone can be found on the surface of the implant about 1 week after its installation; the bone remodeling begins between 6 and 12 weeks and continues throughout life. After the implant insertion, depending on the energy of the surface, the plasma fluid immediately adheres, in close contact with the surface, promoting the adsorption of proteins and inducing the indirect interaction of the cells with the material. Macrophages are cells found in the tissues and originated from bone marrow monocytes. The M1 macrophages orchestrate the phagocytic phase in the inflammatory region and also produce inflammatory cytokines involved with the chronic inflammation and the cleaning of the wound and damaged tissues from bacteria. On the other hand, alternative-activated macrophages (M2) are activated by IL-10, the immune complex. Its main function consists on regulating negatively the inflammation through the secretion of the immunosuppressant IL-10. The M2 macrophages present involvement with the immunosuppression, besides having a low capacity for presenting antigens and high production of cytokines; these can be further divided into M2a, M2b, and M2c, based on the gene expression profile.

scholarly journals Micro/nano-textured hierarchical titanium topography promotes exosome biogenesis and secretion to improve osseointegration

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Zhengchuan Zhang ◽  
Ruogu Xu ◽  
Yang Yang ◽  
Chaoan Liang ◽  
Xiaolin Yu ◽  
...  
Keyword(s):  
Gene Expression ◽  
Titanium Surface ◽  
Titanium Implants ◽  
Extracellular Secretion ◽  
Exosome Biogenesis ◽  
Marrow Mesenchymal Stem Cells ◽  
Proliferation In Vitro ◽  
Extracellular Environment

Abstract Background Micro/nano-textured hierarchical titanium topography is more bioactive and biomimetic than smooth, micro-textured or nano-textured titanium topographies. Bone marrow mesenchymal stem cells (BMSCs) and exosomes derived from BMSCs play important roles in the osseointegration of titanium implants, but the effects and mechanisms of titanium topography on BMSCs-derived exosome secretion are still unclear. This study determined whether the secretion behavior of exosomes derived from BMSCs is differently affected by different titanium topographies both in vitro and in vivo. Results We found that both micro/nanonet-textured hierarchical titanium topography and micro/nanotube-textured hierarchical titanium topography showed favorable roughness and hydrophilicity. These two micro/nano-textured hierarchical titanium topographies enhanced the spreading areas of BMSCs on the titanium surface with stronger promotion of BMSCs proliferation in vitro. Compared to micro-textured titanium topography, micro/nano-textured hierarchical titanium topography significantly enhanced osseointegration in vivo and promoted BMSCs to synthesize and transport exosomes and then release these exosomes into the extracellular environment both in vitro and in vivo. Moreover, micro/nanonet-textured hierarchical titanium topography promoted exosome secretion by upregulating RAB27B and SMPD3 gene expression and micro/nanotube-textured hierarchical titanium topography promoted exosome secretion due to the strongest enhancement in cell proliferation. Conclusions These findings provide evidence that micro/nano-textured hierarchical titanium topography promotes exosome biogenesis and extracellular secretion for enhanced osseointegration. Our findings also highlight that the optimized titanium topography can increase exosome secretion from BMSCs, which may promote osseointegration of titanium implants.

Gene expression in human osteoblastic cells from normal and heterotopic ossification

2004 ◽  
Vol 76 (1) ◽  
pp. 37-43 ◽  
Author(s):  
Christophe Chauveau ◽  
Jean-Christophe Devedjian ◽  
Marie-Claude Blary ◽  
Christophe Delecourt ◽  
Pierre Hardouin ◽  
...  
Keyword(s):  
Gene Expression ◽  
Heterotopic Ossification ◽  
Osteoblastic Cells ◽  
Human Osteoblastic Cells

Selective adhesion of osteoblastic cells to different integrin ligands induces osteopontin gene expression

2003 ◽  
Vol 22 (3) ◽  
pp. 241-249 ◽  
Author(s):  
R.S. Carvalho ◽  
P.J. Kostenuik ◽  
E. Salih ◽  
A. Bumann ◽  
L.C. Gerstenfeld
Keyword(s):  
Gene Expression ◽  
Osteoblastic Cells ◽  
Integrin Ligands

Behavior of MC3T3-E1 Osteoblastic Cells Cultured on Titanium and Zirconia Surfaces

2017 ◽  
Vol 26 (3) ◽  
pp. 373-377 ◽  
Author(s):  
Ricardo Gapski ◽  
Elizabeth Ferreira Martinez
Keyword(s):  
Osteoblastic Cells ◽  
Cells Cultured
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