scholarly journals Effect of low-magnitude, high-frequency vibration on osteocytes in the regulation of osteoclasts

Bone ◽  
2010 ◽  
Vol 46 (6) ◽  
pp. 1508-1515 ◽  
Author(s):  
Esther Lau ◽  
Saja Al-Dujaili ◽  
Axel Guenther ◽  
Dawei Liu ◽  
Liyun Wang ◽  
...  
Keyword(s):  
High Frequency ◽  
High Frequency Vibration ◽  
Low Magnitude

Multi-Level Assessment of Fracture Calluses in Rats Subjected to Low-Magnitude High-Frequency Vibration with Different Rest Periods

2016 ◽  
Vol 44 (8) ◽  
pp. 2489-2504 ◽  
Author(s):  
Jiazi Gao ◽  
He Gong ◽  
Xu Huang ◽  
Rui Zhang ◽  
Renshi Ma ◽  
...  
Keyword(s):  
High Frequency ◽  
High Frequency Vibration ◽  
Rest Periods ◽  
Multi Level ◽  
Low Magnitude

scholarly journals Primary cilia respond to intermittent low-magnitude, high-frequency vibration and mediate vibration-induced effects in osteoblasts

2020 ◽  
Vol 318 (1) ◽  
pp. C73-C82 ◽  
Author(s):  
Yan-Hui Li ◽  
Dong Zhu ◽  
Zongbing Cao ◽  
Yanwei Liu ◽  
Jian Sun ◽  
...  
Keyword(s):  
Western Blot ◽  
Blot Analysis ◽  
High Frequency ◽  
Western Blot Analysis ◽  
Primary Cilia ◽  
Critical Role ◽  
Rt Pcr ◽  
High Frequency Vibration ◽  
Low Magnitude

Our objective was to investigate the role of primary cilia in low-magnitude, high-frequency vibration (LMHFV) treatment of MC3T3-E1 osteoblasts (OBs). We used chloral hydrate (CH), which has a well-characterized function in chemically removing primary cilia, to elucidate the role of primary cilia in LMHFV-induced OB osteogenic responses through cell viability assay, Western blot analysis, real-time quantitative RT-PCR, and histochemical staining methods. We observed a significant, 30% decrease in the number of MC3T3-E1 OBs with primary cilia (reduced from 64.3 ± 5%) and an approximately 50% reduction in length of primary cilia (reduced from 3 ± 0.8 μm) after LMHFV stimulation. LMHFV stimulation upregulated protein expression of the bone matrix markers collagen 1 (COL-1), osteopontin (OPN), and osteoclacin(OCN) in MC3T3-E1 OBs, indicating that LMHFV induces osteogenesis. High-concentration or long-duration CH exposure resulted in inhibition of MC3T3-E1 OB survival. In addition, Western blot analysis and RT-PCR revealed that CH treatment prevented LMHFV-induced osteogenesis. Furthermore, decreased alkaline phosphate activity, reduced OB differentiation, mineralization, and maturation were observed in CH-pretreated and LMHFV-treated OBs. We showed that LMHFV induces morphological changes in primary cilia that may fine-tune their mechanosensitivity. In addition, we demonstrated the significant inhibition by CH of LMHFV-induced OB mineralization, maturation, and differentiation, which might reveal the critical role of primary cilia in the process.

scholarly journals Low-magnitude high-frequency vibration (LMHFV) enhances bone remodeling in osteoporotic rat femoral fracture healing

2010 ◽  
Vol 29 (5) ◽  
pp. 746-752 ◽  
Author(s):  
Dick Ho-Kiu Chow ◽  
Kwok-Sui Leung ◽  
Ling Qin ◽  
Andraay Hon-Chi Leung ◽  
Wing-Hoi Cheung
Keyword(s):  
Fracture Healing ◽  
Bone Remodeling ◽  
Femoral Fracture ◽  
High Frequency ◽  
High Frequency Vibration ◽  
Low Magnitude
Sign in / Sign up

Export Citation Format

Share Document