The role of Akt1 in terminal stages of endochondral bone formation: Angiogenesis and ossification

Bone ◽  
2009 ◽  
Vol 45 (6) ◽  
pp. 1133-1145 ◽  
Author(s):  
Veronica Ulici ◽  
Katie D. Hoenselaar ◽  
Hanga Agoston ◽  
David D. McErlain ◽  
Joseph Umoh ◽  
...  
Keyword(s):  
Bone Formation ◽  
Endochondral Bone Formation ◽  
Endochondral Bone

scholarly journals Expression and Role of IL-1β Signaling in Chondrocytes Associated with Retinoid Signaling during Fracture Healing

2020 ◽  
Vol 21 (7) ◽  
pp. 2365 ◽  
Author(s):  
Tsuyoshi Shimo ◽  
Hiroaki Takebe ◽  
Tatsuo Okui ◽  
Yuki Kunisada ◽  
Soichiro Ibaragi ◽  
...  
Keyword(s):  
Bone Formation ◽  
Fracture Healing ◽  
P38 Mapk ◽  
Inverse Agonist ◽  
Endochondral Bone Formation ◽  
Dependent Manner ◽  
Retinoid Receptor ◽  
Endochondral Bone ◽  
Inverse Agonists

The process of fracture healing consists of an inflammatory reaction and cartilage and bone tissue reconstruction. The inflammatory cytokine interleukin-1β (IL-1β) signal is an important major factor in fracture healing, whereas its relevance to retinoid receptor (an RAR inverse agonist, which promotes endochondral bone formation) remains unclear. Herein, we investigated the expressions of IL-1β and retinoic acid receptor gamma (RARγ) in a rat fracture model and the effects of IL-1β in the presence of one of several RAR inverse agonists on chondrocytes. An immunohistochemical analysis revealed that IL-1β and RARγ were expressed in chondrocytes at the fracture site in the rat ribs on day 7 post-fracture. In chondrogenic ATDC5 cells, IL-1β decreases the levels of aggrecan and type II collagen but significantly increased the metalloproteinase-13 (Mmp13) mRNA by real-time reverse transcription-polymerase chain reaction (RT-PCR) analysis. An RAR inverse agonist (AGN194310) inhibited IL-1β-stimulated Mmp13 and Ccn2 mRNA in a dose-dependent manner. Phosphorylated extracellular signal regulated-kinases (pERK1/2) and p-p38 mitogen-activated protein kinase (MAPK) were increased time-dependently by IL-1β treatment, and the IL-1β-induced p-p38 MAPK was inhibited by AGN194310. Experimental p38 inhibition led to a drop in the IL-1β-stimulated expressions of Mmp13 and Ccn2 mRNA. MMP13, CCN2, and p-p38 MAPK were expressed in hypertrophic chondrocytes near the invaded vascular endothelial cells. As a whole, these results point to role of the IL-1β via p38 MAPK as important signaling in the regulation of the endochondral bone formation in fracture healing, and to the actions of RAR inverse agonists as potentially relevant modulators of this process.

The Role of Microtubule in the Regulation of Endochondral Bone Formation in the Developing Mouse Cranial Base

2011 ◽  
Vol 24 (2) ◽  
pp. 105
Author(s):  
Hyuk Jae Kwon ◽  
Jong Min Lee ◽  
Kyoung Won Cho ◽  
Jeong Oh Shin ◽  
Sung Won Cho ◽  
...  
Keyword(s):  
Bone Formation ◽  
Cranial Base ◽  
Endochondral Bone Formation ◽  
Endochondral Bone

scholarly journals 063 THE ROLE OF AKT1 IN TERMINAL STAGES OF ENDOCHONDRAL BONE FORMATION: ANGIOGENESIS AND OSSIFICATION

2009 ◽  
Vol 17 ◽  
pp. S43
Author(s):  
V. Ulici ◽  
K.D. Hoenselaar ◽  
H. Agoston ◽  
D.D. McErlain ◽  
J. Umoh ◽  
...  
Keyword(s):  
Bone Formation ◽  
Endochondral Bone Formation ◽  
Endochondral Bone

scholarly journals Role of interleukin-10 in endochondral bone formation

2013 ◽  
Vol 21 ◽  
pp. S129
Author(s):  
G. Kim ◽  
Y.-K. Jung ◽  
H.-R. Park ◽  
E.-J. Lee ◽  
J.-Y. Choi ◽  
...  
Keyword(s):  
Bone Formation ◽  
Interleukin 10 ◽  
Endochondral Bone Formation ◽  
Endochondral Bone

scholarly journals On the role of type IX collagen in the extracellular matrix of cartilage: type IX collagen is localized to intersections of collagen fibrils.

1986 ◽  
Vol 102 (5) ◽  
pp. 1931-1939 ◽  
Author(s):  
W Müller-Glauser ◽  
B Humbel ◽  
M Glatt ◽  
P Sträuli ◽  
K H Winterhalter ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Bone Formation ◽  
Tissue Distribution ◽  
Chicken Embryo ◽  
Polyclonal Antibodies ◽  
Type Ii Collagen ◽  
Endochondral Bone Formation ◽  
Type Ii ◽  
Endochondral Bone

The tissue distribution of type II and type IX collagen in 17-d-old chicken embryo was studied by immunofluorescence using polyclonal antibodies against type II collagen and a peptic fragment of type IX collagen (HMW), respectively. Both proteins were found only in cartilage where they were co-distributed. They occurred uniformly throughout the extracellular matrix, i.e., without distinction between pericellular, territorial, and interterritorial matrices. Tissues that undergo endochondral bone formation contained type IX collagen, whereas periosteal and membranous bones were negative. The thin collagenous fibrils in cartilage consisted of type II collagen as determined by immunoelectron microscopy. Type IX collagen was associated with the fibrils but essentially was restricted to intersections of the fibrils. These observations suggested that type IX collagen contributes to the stabilization of the network of thin fibers of the extracellular matrix of cartilage by interactions of its triple helical domains with several fibrils at or close to their intersections.

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