Subchondral bone regeneration in osteochondral defect After chondrocyte pellet implantation

Bone ◽  
2009 ◽  
Vol 44 ◽  
pp. S264-S265
Author(s):  
M.W.N. Wong ◽  
Y.C. Cheuk ◽  
K.M. Lee ◽  
S.C. Fu ◽  
L. Qin
Keyword(s):  
Bone Regeneration ◽  
Subchondral Bone ◽  
Osteochondral Defect

scholarly journals Effect of BMP-2 with Microfracture on Osteochondral Defect in a Rabbits Talus

2019 ◽  
Vol 4 (4) ◽  
pp. 2473011419S0015
Author(s):  
Le Hoang Nam Dang ◽  
Kwang-Bok Lee
Keyword(s):  
Bone Regeneration ◽  
Subchondral Bone ◽  
Collagen Type ◽  
Osteochondral Defect ◽  
Border Zone ◽  
Micro Ct ◽  
Full Thickness ◽  
Group I ◽  
Defect Repair

Category: Ankle, Basic Sciences/Biologics Introduction/Purpose: Microfracture is a technically safe, simple and cost-effective treatment option for osteochondral defect of the talus. However, fibrous tissue and fibrocartilage represent the predominant repairing tissues derived from these procedures and these tissues types do not exhibit the mechanical properties of native hyaline cartilage, long time for cartilage reconstruction and long-term failure results. Bone morphogenetic protein-2 (BMP-2) has been shown to stimulate matrix synthesis and increase in synthesis of cartilage-specific collagen type IIB thereby enhance regeneration of articular cartilage in vitro. The purpose of this study is to evaluate the treatment ability of combining BMP-2 with microfracture on osteochondral defect in rabbit talus. Methods: Full-thickness chondral defects (3 x 3 x 2 mm) was created in the center talus dome. Twenty four male white New Zealand rabbits were divided into four groups of 6 animals into each group dependent on treatment method; Group I (control, no treatment), II (microfracture only), III (10 µL rhBMP-2 treatment), IV (microfracture combine with 10 µL rhBMP-2 treatment). The animals were sacrificed at 2, 4, 6 weeks post operatively. The macroscopic assessment of the repaired tissue was evaluated using the International Cartilage Repair Society (ICRS) Macroscopic Score, which considers the degree of defect repair, the integration to the border zone, and the macroscopic appearance. Subchondral bone regeneration on defect was evaluated by micro-CT scan and the histological findings were scored by using a modified version of the established scoring system for osteochondral repair described by Wakitani, immunohistochemical staining was used for analysis of collagen type I and II. Results: : Excessive bone formation that originated from the subchondral bone region was not observed in any sample. Micro-CT shows higher subchondral bone regeneration on defect in group III, IV than group I, II at 4 and 6 weeks, respectively. According to macroscopic and histological results, group V shows higher quality of cartilage and faster cartilage regeneration compare with another group. In immunohistochemical analysis, group IV shows stronger positive staining for collagen type II in the area of the defect. Conclusion: These findings indicate that the combination BMP-2 with microfracture is effective at accelerating and improving quality of full-thickness cartilage defect repair in a rabbit talus model.

scholarly journals Micro-Computed Tomography Analysis of Subchondral Bone Regeneration Using Osteochondral Scaffolds in an Ovine Condyle Model

2021 ◽  
Vol 11 (3) ◽  
pp. 891
Author(s):  
Taylor Flaherty ◽  
Maryam Tamaddon ◽  
Chaozong Liu
Keyword(s):  
Computed Tomography ◽  
Bone Regeneration ◽  
Subchondral Bone ◽  
Volume Ratio ◽  
Bone Volume ◽  
Osteochondral Defects ◽  
Micro Ct ◽  
Micro Computed Tomography ◽  
Trabecular Thickness ◽  
Osteochondral Scaffold

Osteochondral scaffold technology has emerged as a promising therapy for repairing osteochondral defects. Recent research suggests that seeding osteochondral scaffolds with bone marrow concentrate (BMC) may enhance tissue regeneration. To examine this hypothesis, this study examined subchondral bone regeneration in scaffolds with and without BMC. Ovine stifle condyle models were used for the in vivo study. Two scaffold systems (8 mm diameter and 10 mm thick) with and without BMC were implanted into the femoral condyle, and the tissues were retrieved after six months. The retrieved femoral condyles (with scaffold in) were examined using micro-computed tomography scans (micro-CT), and the micro-CT data were further analysed by ImageJ with respect to trabecular thickness, bone volume to total volume ratio (BV/TV) ratio, and degree of anisotropy of bone. Statistical analysis compared bone regeneration between scaffold groups and sub-set regions. These results were mostly insignificant (p < 0.05), with the exception of bone volume to total volume ratio when comparing scaffold composition and sub-set region. Additional trends in the data were observed. These results suggest that the scaffold composition and addition of BMC did not significantly affect bone regeneration in osteochondral defects after six months. However, this research provides data which may guide the development of future treatments.

scholarly journals Potential Implantable Nanofibrous Biomaterials Combined with Stem Cells for Subchondral Bone Regeneration

Materials ◽  
2020 ◽  
Vol 13 (14) ◽  
pp. 3087
Author(s):  
Rana Smaida ◽  
Luc Pijnenburg ◽  
Silvia Irusta ◽  
Erico Himawan ◽  
Gracia Mendoza ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Regeneration ◽  
Subchondral Bone ◽  
Therapeutic Potential ◽  
Sodium Hyaluronate ◽  
Vinyl Pyrrolidone ◽  
Regenerative Ability ◽  
Poly Vinyl Pyrrolidone

The treatment of osteochondral defects remains a challenge. Four scaffolds were produced using Food and Drug Administration (FDA)-approved polymers to investigate their therapeutic potential for the regeneration of the osteochondral unit. Polycaprolactone (PCL) and poly(vinyl-pyrrolidone) (PVP) scaffolds were made by electrohydrodynamic techniques. Hydroxyapatite (HAp) and/or sodium hyaluronate (HA) can be then loaded to PCL nanofibers and/or PVP particles. The purpose of adding hydroxyapatite and sodium hyaluronate into PCL/PVP scaffolds is to increase the regenerative ability for subchondral bone and joint cartilage, respectively. Human bone marrow-derived mesenchymal stem cells (hBM-MSCs) were seeded on these biomaterials. The biocompatibility of these biomaterials in vitro and in vivo, as well as their potential to support MSC differentiation under specific chondrogenic or osteogenic conditions, were evaluated. We show here that hBM-MSCs could proliferate and differentiate both in vitro and in vivo on these biomaterials. In addition, the PCL-HAp could effectively increase the mineralization and induce the differentiation of MSCs into osteoblasts in an osteogenic condition. These results indicate that PCL-HAp biomaterials combined with MSCs could be a beneficial candidate for subchondral bone regeneration.

Patient-specific Scaffolds with a Biomimetic Gradient Environment for Articular Cartilage–Subchondral Bone Regeneration

2020 ◽  
Vol 3 (8) ◽  
pp. 4820-4831
Author(s):  
Cuidi Li ◽  
Kan Wang ◽  
Tao Li ◽  
Xiaojun Zhou ◽  
Zhenjiang Ma ◽  
...  
Keyword(s):  
Articular Cartilage ◽  
Bone Regeneration ◽  
Subchondral Bone ◽  
Patient Specific ◽  
Gradient Environment

Poly-N-Acetyl Glucosamine-SO4 for Repairing Osteochondral Defect in Rabbits

2005 ◽  
Vol 288-289 ◽  
pp. 83-86 ◽  
Author(s):  
Qian K. Kang ◽  
Christopher M. Hill ◽  
Marina V. Demcheva ◽  
John Vournakis ◽  
Yuehuei H. An
Keyword(s):  
Animal Models ◽  
Subchondral Bone ◽  
Osteochondral Defect ◽  
Water Soluble ◽  
Osteochondral Defects ◽  
Femoral Trochlea ◽  
Good Healing

Repair of osteochondral defects created in rabbit femoral trochlea were studied using a water-soluble sulfated p-GlcNAc formulation, (Marine Polymer Technologies, Danvers, MA, USA). After 12 weeks of healing empty defects were compared to defects filled with sulfated p-GlcNAc sponge alone and sulfated p-GlcNAc sponge seeded with autologous chondrocytes. The chondrocyte seeded sponge provided the best healing of both cartilage and subchondral bone. The sulfated p-GlcNAc sponge alone did not provide as good healing as the chondrocyte seeded sponge, but healing was still superior to that of the empty defect. This study supports the use of p-GlcNAc sponge to augment healing of osteochondral defects in animal models.

scholarly journals Role of Mesenchymal Stem Cells Densities When Injected as Suspension in Joints with Osteochondral Defects

Cartilage ◽  
2017 ◽  
Vol 10 (1) ◽  
pp. 61-69 ◽  
Author(s):  
Elhussein Elbadry Mahmoud ◽  
Naosuke Kamei ◽  
Goki Kamei ◽  
Tomoyuki Nakasa ◽  
Ryo Shimizu ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Subchondral Bone ◽  
Osteochondral Defect ◽  
Deep Layer ◽  
Femoral Condyle ◽  
Fibrous Tissue ◽  
Intraarticular Injection ◽  
Control Group ◽  
Osteochondral Repair

Objective The aim of this study was to evaluate an intraarticular injection of different doses of autologous mesenchymal stem cells (MSCs) for improving repair of midterm osteochondral defect. Design At 4 weeks postoperative marrow stimulation model bilaterally (3 mm diameter; 4 mm depth) in the medial femoral condyle, autologous MSCs were injected into knee joint. Twenty-four Japanese rabbits aged 6 months were divided randomly into 4 groups ( n = 6 per group): the control group and and MSC groups including 0.125, 1.25, and 6.25 million MSCs. Repaired tissue was assessed macroscopically and histologically at 4 and 12 weeks after intraarticular injection of MSCs. Results At 12 weeks, there was no repair tissue in the control group. The gross appearance of the 1.25 and 6.25 million MSC groups revealed complete repair of the defect with white to pink tissue at 12 weeks. An osteochondral repair was histologically significantly better in the 1.25 and 6.25 million MSC groups than in the control and 0.125 million MSC groups at 4 and 12 weeks, due to presence of hyaline-like tissue in the deep layer at 4 weeks, and at 12 weeks hyaline cartilage formation at the periphery and fibrous tissue containing some chondrocytes in the deep layer of the center of the defect. Subchondral bone was restructured in the 1.25 and 6.25 million MSC groups, although it did not resemble the normal bone. Conclusion An intraarticular injection of 1.25 or 6.25 million MSCs could promote the repair of subchondral bone, even in the case of midterm osteochondral defect.

Positive Effect of Alendronate on Subchondral Bone Healing and Subsequent Cartilage Repair in a Rabbit Osteochondral Defect Model

2009 ◽  
Vol 37 (1_suppl) ◽  
pp. 139-147 ◽  
Author(s):  
Kohei Nishitani ◽  
Takaaki Shirai ◽  
Masahiko Kobayashi ◽  
Hiroshi Kuroki ◽  
Yoshiaki Azuma ◽  
...  
Keyword(s):  
Cartilage Repair ◽  
Subchondral Bone ◽  
Bone Healing ◽  
Osteochondral Defect ◽  
Defect Model ◽  
Positive Effect
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