In vivo biocompatibility of ultra-short single-walled carbon nanotube/biodegradable polymer nanocomposites for bone tissue engineering

Bone ◽  
2008 ◽  
Vol 43 (2) ◽  
pp. 362-370 ◽  
Author(s):  
Balaji Sitharaman ◽  
Xinfeng Shi ◽  
X. Frank Walboomers ◽  
Hongbing Liao ◽  
Vincent Cuijpers ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Carbon Nanotube ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Polymer Nanocomposites ◽  
Biodegradable Polymer ◽  
Single Walled Carbon Nanotube ◽  
Single Walled Carbon

Amine-functionalized Single-walled Carbon Nanotube/Polycaprolactone Electrospun Scaffold for Bone Tissue Engineering: in vitro Study

2019 ◽  
Vol 20 (9) ◽  
pp. 1869-1882 ◽  
Author(s):  
Hadi Tohidlou ◽  
Seyedeh Sara Shafiei ◽  
Shahsanam Abbasi ◽  
Mitra Asadi-Eydivand ◽  
Mehrnoosh Fathi-Roudsari
Keyword(s):  
Tissue Engineering ◽  
Carbon Nanotube ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
In Vitro Study ◽  
Electrospun Scaffold ◽  
Single Walled Carbon Nanotube ◽  
Single Walled Carbon ◽  
Amine Functionalized

scholarly journals Single walled carbon nanotube composites for bone tissue engineering

2013 ◽  
Vol 31 (9) ◽  
pp. 1374-1381 ◽  
Author(s):  
Ashim Gupta ◽  
Mia D. Woods ◽  
Kenneth David Illingworth ◽  
Ryan Niemeier ◽  
Isaac Schafer ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Carbon Nanotube ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Single Walled Carbon Nanotube ◽  
Single Walled Carbon ◽  
Nanotube Composites ◽  
Carbon Nanotube Composites

scholarly journals Fabrication of porous ultra-short single-walled carbon nanotube nanocomposite scaffolds for bone tissue engineering

Biomaterials ◽  
2007 ◽  
Vol 28 (28) ◽  
pp. 4078-4090 ◽  
Author(s):  
Xinfeng Shi ◽  
Balaji Sitharaman ◽  
Quynh P. Pham ◽  
Feng Liang ◽  
Katherine Wu ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Carbon Nanotube ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Single Walled Carbon Nanotube ◽  
Single Walled Carbon ◽  
Nanocomposite Scaffolds

In vitrocytotoxicity of single‐walled carbon nanotube/biodegradable polymer nanocomposites

2008 ◽  
Vol 86A (3) ◽  
pp. 813-823 ◽  
Author(s):  
Xinfeng Shi ◽  
Balaji Sitharaman ◽  
Quynh P. Pham ◽  
Patrick P. Spicer ◽  
Jared L. Hudson ◽  
...  
Keyword(s):  
Carbon Nanotube ◽  
Polymer Nanocomposites ◽  
Biodegradable Polymer ◽  
Single Walled Carbon Nanotube ◽  
Single Walled Carbon

scholarly journals A novel gelatin/chitooligosaccharide/demineralized bone matrix composite scaffold and periosteum-derived mesenchymal stem cells for bone tissue engineering

2021 ◽  
Vol 25 (1) ◽  
Author(s):  
Thakoon Thitiset ◽  
Siriporn Damrongsakkul ◽  
Supansa Yodmuang ◽  
Wilairat Leeanansaksiri ◽  
Jirun Apinun ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Bone Formation ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Demineralized Bone Matrix ◽  
Bone Matrix ◽  
Alp Activity

Abstract Background A novel biodegradable scaffold including gelatin (G), chitooligosaccharide (COS), and demineralized bone matrix (DBM) could play a significant part in bone tissue engineering. The present study aimed to investigate the biological characteristics of composite scaffolds in combination of G, COS, and DBM for in vitro cell culture and in vivo animal bioassays. Methods Three-dimensional scaffolds from the mixture of G, COS, and DBM were fabricated into 3 groups, namely, G, GC, and GCD using a lyophilization technique. The scaffolds were cultured with mesenchymal stem cells (MSCs) for 4 weeks to determine biological responses such as cell attachment and cell proliferation, alkaline phosphatase (ALP) activity, calcium deposition, cell morphology, and cell surface elemental composition. For the in vivo bioassay, G, GC, and GCD, acellular scaffolds were implanted subcutaneously in 8-week-old male Wistar rats for 4 weeks and 8 weeks. The explants were assessed for new bone formation using hematoxylin and eosin (H&E) staining and von Kossa staining. Results The MSCs could attach and proliferate on all three groups of scaffolds. Interestingly, the ALP activity of MSCs reached the greatest value on day 7 after cultured on the scaffolds, whereas the calcium assay displayed the highest level of calcium in MSCs on day 28. Furthermore, weight percentages of calcium and phosphorus on the surface of MSCs after cultivation on the GCD scaffolds increased when compared to those on other scaffolds. The scanning electron microscopy images showed that MSCs attached and proliferated on the scaffold surface thoroughly over the cultivation time. Mineral crystal aggregation was evident in GC and greatly in GCD scaffolds. H&E staining illustrated that G, GC, and GCD scaffolds displayed osteoid after 4 weeks of implantation and von Kossa staining confirmed the mineralization at 8 weeks in G, GC, and GCD scaffolds. Conclusion The MSCs cultured in GCD scaffolds revealed greater osteogenic differentiation than those cultured in G and GC scaffolds. Additionally, the G, GC, and GCD scaffolds could promote in vivo ectopic bone formation in rat model. The GCD scaffolds exhibited maximum osteoinductive capability compared with others and may be potentially used for bone regeneration.

scholarly journals Heidelberg-mCT-Analyzer: a novel method for standardized microcomputed-tomography-guided evaluation of scaffold properties in bone and tissue research

2015 ◽  
Vol 2 (11) ◽  
pp. 150496 ◽  
Author(s):  
Fabian Westhauser ◽  
Christian Weis ◽  
Melanie Hoellig ◽  
Tyler Swing ◽  
Gerhard Schmidmaier ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Three Dimensional ◽  
Characteristic Parameter ◽  
Micro Computed Tomography ◽  
Mineral Density ◽  
Independent Analysis ◽  
Scaffold Properties

Bone tissue engineering and bone scaffold development represent two challenging fields in orthopaedic research. Micro-computed tomography (mCT) allows non-invasive measurement of these scaffolds’ properties in vivo . However, the lack of standardized mCT analysis protocols and, therefore, the protocols’ user-dependency make interpretation of the reported results difficult. To overcome these issues in scaffold research, we introduce the Heidelberg-mCT-Analyzer. For evaluation of our technique, we built 10 bone-inducing scaffolds, which underwent mCT acquisition before ectopic implantation (T0) in mice, and at explantation eight weeks thereafter (T1). The scaffolds’ three-dimensional reconstructions were automatically segmented using fuzzy clustering with fully automatic level-setting. The scaffold itself and its pores were then evaluated for T0 and T1. Analysing the scaffolds’ characteristic parameter set with our quantification method showed bone formation over time. We were able to demonstrate that our algorithm obtained the same results for basic scaffold parameters (e.g. scaffold volume, pore number and pore volume) as other established analysis methods. Furthermore, our algorithm was able to analyse more complex parameters, such as pore size range, tissue mineral density and scaffold surface. Our imaging and post-processing strategy enables standardized and user-independent analysis of scaffold properties, and therefore is able to improve the quantitative evaluations of scaffold-associated bone tissue-engineering projects.

Investigation of synergistic effects of inductive and conductive factors in gelatin-based cryogels for bone tissue engineering

2016 ◽  
Vol 4 (10) ◽  
pp. 1827-1841 ◽  
Author(s):  
Han-Tsung Liao ◽  
K. T. Shalumon ◽  
Kun-Hung Chang ◽  
Chialin Sheu ◽  
Jyh-Ping Chen
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Synergistic Effects

Gelatin cryogels modified with nHAP and BMP-2 could provide cues to promote the osteogenesis of ADSCs in vitro and in vivo.

scholarly journals Protocol of Co-Culture of Human Osteoblasts and Osteoclasts to Test Biomaterials for Bone Tissue Engineering

2022 ◽  
Vol 5 (1) ◽  
pp. 8
Author(s):  
Giorgia Borciani ◽  
Giorgia Montalbano ◽  
Nicola Baldini ◽  
Chiara Vitale-Brovarone ◽  
Gabriela Ciapetti
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Bone Cells ◽  
Bone Cell ◽  
Seeding Density ◽  
Bone Homeostasis ◽  
Bone Microenvironment

New biomaterials and scaffolds for bone tissue engineering (BTE) applications require to be tested in a bone microenvironment reliable model. On this assumption, the in vitro laboratory protocols with bone cells represent worthy experimental systems improving our knowledge about bone homeostasis, reducing the costs of experimentation. To this day, several models of the bone microenvironment are reported in the literature, but few delineate a protocol for testing new biomaterials using bone cells. Herein we propose a clear protocol to set up an indirect co-culture system of human-derived osteoblasts and osteoclast precursors, providing well-defined criteria such as the cell seeding density, cell:cell ratio, the culture medium, and the proofs of differentiation. The material to be tested may be easily introduced in the system and the cell response analyzed. The physical separation of osteoblasts and osteoclasts allows distinguishing the effects of the material onto the two cell types and to evaluate the correlation between material and cell behavior, cell morphology, and adhesion. The whole protocol requires about 4 to 6 weeks with an intermediate level of expertise. The system is an in vitro model of the bone remodeling system useful in testing innovative materials for bone regeneration, and potentially exploitable in different application fields. The use of human primary cells represents a close replica of the bone cell cooperation in vivo and may be employed as a feasible system to test materials and scaffolds for bone substitution and regeneration.

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