Characterizing the BMP pathway in a wild type mouse model of distraction osteogenesis

Bone ◽  
2008 ◽  
Vol 42 (6) ◽  
pp. 1144-1153 ◽  
Author(s):  
Tasima Haque ◽  
Fares Hamade ◽  
Norine Alam ◽  
Maria Kotsiopriftis ◽  
Dominique Lauzier ◽  
...  
Keyword(s):  
Mouse Model ◽  
Distraction Osteogenesis ◽  
Wild Type Mouse ◽  
Wild Type ◽  
Bmp Pathway

scholarly journals Sequencing of Historical Isolates, K-mer Mining and High Serological Cross-Reactivity with Ross River Virus Argue against the Presence of Getah Virus in Australia

Pathogens ◽  
2020 ◽  
Vol 9 (10) ◽  
pp. 848
Author(s):  
Daniel J. Rawle ◽  
Wilson Nguyen ◽  
Troy Dumenil ◽  
Rhys Parry ◽  
David Warrilow ◽  
...  
Keyword(s):  
Mouse Model ◽  
Sequence Data ◽  
Wild Type Mouse ◽  
Cross Reactivity ◽  
Cross Protection ◽  
Ross River Virus ◽  
Wild Type ◽  
Serological Relationship ◽  
Sequence Read Archive ◽  
Getah Virus

Getah virus (GETV) is a mosquito-transmitted alphavirus primarily associated with disease in horses and pigs in Asia. GETV was also reported to have been isolated from mosquitoes in Australia in 1961; however, retrieval and sequencing of the original isolates (N544 and N554), illustrated that these viruses were virtually identical to the 1955 GETVMM2021 isolate from Malaysia. K-mer mining of the >40,000 terabases of sequence data in the Sequence Read Archive followed by BLASTn confirmation identified multiple GETV sequences in biosamples from Asia (often as contaminants), but not in biosamples from Australia. In contrast, sequence reads aligning to the Australian Ross River virus (RRV) were readily identified in Australian biosamples. To explore the serological relationship between GETV and other alphaviruses, an adult wild-type mouse model of GETV was established. High levels of cross-reactivity and cross-protection were evident for convalescent sera from mice infected with GETV or RRV, highlighting the difficulties associated with the interpretation of early serosurveys reporting GETV antibodies in Australian cattle and pigs. The evidence that GETV circulates in Australia is thus not compelling.

scholarly journals Transplantation of wild-type mouse hematopoietic stem and progenitor cells ameliorates deficits in a mouse model of Friedreich’s ataxia

2017 ◽  
Vol 9 (413) ◽  
pp. eaaj2347 ◽  
Author(s):  
Celine J. Rocca ◽  
Spencer M. Goodman ◽  
Jennifer N. Dulin ◽  
Joseph H. Haquang ◽  
Ilya Gertsman ◽  
...  
Keyword(s):  
Mouse Model ◽  
Progenitor Cells ◽  
Wild Type Mouse ◽  
Friedreich’S Ataxia ◽  
Friedreich's Ataxia ◽  
Wild Type ◽  
Hematopoietic Stem ◽  
Stem And Progenitor Cells

Intravesical anti-PD-1 immune checkpoint inhibition in urothelial bladder cancer in a mouse model.

2020 ◽  
Vol 38 (6_suppl) ◽  
pp. 537-537
Author(s):  
Anne Rajkumar ◽  
Jian Wang ◽  
Kevin E. Neuzil ◽  
Austin Noah Kirschner ◽  
Sam S. Chang
Keyword(s):  
Bladder Cancer ◽  
Mouse Model ◽  
Immune Checkpoint ◽  
Intraperitoneal Administration ◽  
Wild Type Mouse ◽  
Wild Type ◽  
Urothelial Bladder Cancer ◽  
Intravesical Administration ◽  
Antibody Treatment ◽  
Urothelial Bladder

537 Background: Non-muscle-invasive bladder cancer is treated by resection within the bladder and bladder instillment with BCG or chemotherapy, but the majority of intermediate- and high-risk cases subsequently recur. Systemic administration of anti-PD-1 immune checkpoint inhibitors (anti-PD-1) are approved treatments for metastatic urothelial bladder cancer. We hypothesized that intravesical instillment with an anti-PD-1 inhibitor would treat localized bladder cancer. Methods: We investigated a syngeneic wild-type mouse model of orthotopic urothelial bladder cancer. We instilled MBT2 cells into wild-type C3H mice to compare treatments, which included weekly intravesical administration of chemotherapy and anti-PD-1 antibody alongside intraperitoneal administration of anti-PD1 antibody. Results: Anti-PD-1 antibody administered by bladder instillment (intravesical route) successfully treats the disease, similarly to anti-PD-1 by systemic route. Anti-PD-1 antibody by either route provides significant survival advantage over isotype control antibody given by bladder instillment. Treatment by immune checkpoint inhibitor increases CD8+ cell infiltration in tumors, particularly when administered intravesically. In addition, antibody treatment avoids toxicity observed for intravesical chemotherapy. Mice who cleared their tumors after initial treatment were rechallenged with tumor engraftment 3-9 months later without any additional treatment, showing initial-anti-PD-1 treated mice did not grow tumors but initial mitomycin-treated mice did grow tumors. Conclusions: Intravesical administration of anti-PD-1 is a promising avenue for treatment of localized bladder cancer, with comparable anti-tumor activity compared to systemic anti-PD-1 in this mouse model.

[18F]-florbetaben PET/CT Imaging in the Alzheimer’s Disease Mouse Model APPswe/PS1dE9

2018 ◽  
Vol 16 (1) ◽  
pp. 49-55 ◽  
Author(s):  
J. Stenzel ◽  
C. Rühlmann ◽  
T. Lindner ◽  
S. Polei ◽  
S. Teipel ◽  
...  
Keyword(s):  
Alzheimer’S Disease ◽  
Alzheimer's Disease ◽  
Mouse Model ◽  
New Drugs ◽  
Imaging Data ◽  
Wild Type ◽  
Preclinical Research ◽  
Standardized Uptake Values ◽  
Pet Ct

Background: Positron-emission-tomography (PET) using 18F labeled florbetaben allows noninvasive in vivo-assessment of amyloid-beta (Aβ), a pathological hallmark of Alzheimer’s disease (AD). In preclinical research, [<sup>18</sup>F]-florbetaben-PET has already been used to test the amyloid-lowering potential of new drugs, both in humans and in transgenic models of cerebral amyloidosis. The aim of this study was to characterize the spatial pattern of cerebral uptake of [<sup>18</sup>F]-florbetaben in the APPswe/ PS1dE9 mouse model of AD in comparison to histologically determined number and size of cerebral Aβ plaques. Methods: Both, APPswe/PS1dE9 and wild type mice at an age of 12 months were investigated by smallanimal PET/CT after intravenous injection of [<sup>18</sup>F]-florbetaben. High-resolution magnetic resonance imaging data were used for quantification of the PET data by volume of interest analysis. The standardized uptake values (SUVs) of [<sup>18</sup>F]-florbetaben in vivo as well as post mortem cerebral Aβ plaque load in cortex, hippocampus and cerebellum were analyzed. Results: Visual inspection and SUVs revealed an increased cerebral uptake of [<sup>18</sup>F]-florbetaben in APPswe/ PS1dE9 mice compared with wild type mice especially in the cortex, the hippocampus and the cerebellum. However, SUV ratios (SUVRs) relative to cerebellum revealed only significant differences in the hippocampus between the APPswe/PS1dE9 and wild type mice but not in cortex; this differential effect may reflect the lower plaque area in the cortex than in the hippocampus as found in the histological analysis. Conclusion: The findings suggest that histopathological characteristics of Aβ plaque size and spatial distribution can be depicted in vivo using [<sup>18</sup>F]-florbetaben in the APPswe/PS1dE9 mouse model.

scholarly journals Effect of Yuzu (Citrus junos) Seed Limonoids and Spermine on Intestinal Microbiota and Hypothalamic Tissue in the Sandhoff Disease Mouse Model

2021 ◽  
Vol 9 (1) ◽  
pp. 17
Author(s):  
Mayumi Minamisawa ◽  
Takuma Suzumura ◽  
Sudeep Bose ◽  
Tetsuyuki Taniai ◽  
Gota Kawai ◽  
...  
Keyword(s):  
Mouse Model ◽  
Intestinal Microbiota ◽  
Neuronal Degeneration ◽  
Sandhoff Disease ◽  
Short Chain Fatty Acids ◽  
Rrna Gene ◽  
Wild Type ◽  
Degrading Bacteria ◽  
Citrus Junos ◽  
Hypothalamic Tissue

The effect of limonoids and spermine (Spm) extracted from yuzu (Citrus junos) seeds on the gut and the brain in a mouse model with Sandhoff disease (SD) was investigated. Wild-type and SD mice were fed a normal diet, or a diet supplemented with limonoid, Spm, or limonoid + Spm for 14–18 weeks, and then 16S rRNA gene amplicon sequencing with extracted DNA from their feces was executed. For SD control mice, intestinal microbiota was mostly composed of Lactobacillus and linked to dysbiosis. For SD and wild-type mice fed with limonoids + Spm or limonoids alone, intestinal microbiota was rich in mucin-degrading bacteria, including Bacteroidetes, Verrucomicrobia, and Firmicutes, and displayed a higher production of short-chain fatty acids and immunoglobulin A. Additionally, SD mice fed with limonoids + Spm or limonoids alone had less inflammation in hypothalamic tissues and displayed a greater number of neurons. Administration of limonoids and/or Spm improved the proportions of beneficial intestinal microbiota to host health and reduced neuronal degeneration in SD mice. Yuzu seed limonoids and Spermine may help to maintain the homeostasis of intestinal microbiota and hypothalamic tissue in the SD mouse model.

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