Regulation of the human tissue-nonspecific alkaline phosphatase gene expression by all--retinoic acid in SaOS-2 osteosarcoma cell line

Bone ◽  
2005 ◽  
Vol 36 (5) ◽  
pp. 866-876 ◽  
Author(s):  
H ORIMO ◽  
T SHIMADA
Keyword(s):  
Gene Expression ◽  
Alkaline Phosphatase ◽  
Retinoic Acid ◽  
Cell Line ◽  
Human Tissue ◽  
Osteosarcoma Cell Line ◽  
Osteosarcoma Cell ◽  
Tissue Nonspecific Alkaline Phosphatase

scholarly journals Effect of Orento, a Traditional Japanese Medicine, on IL-6, IL-8 Secretion, Type 1 Collagen Production and Alkaline Phosphatase Secretion in the Human Osteosarcoma Cell Line Saos-2

Medicines ◽  
2020 ◽  
Vol 7 (10) ◽  
pp. 61
Author(s):  
Hourei Oh ◽  
Kazuya Masuno ◽  
Nobutaka Okusa ◽  
Yoshimasa Makita ◽  
Shin-ichi Fujiwara ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Cell Line ◽  
Osteosarcoma Cell Line ◽  
Osteosarcoma Cell ◽  
Human Osteosarcoma Cell Line ◽  
Collagen Production ◽  
Human Osteosarcoma ◽  
Traditional Japanese Medicine ◽  
Human Osteosarcoma Cell

Background: Orento, a traditional Japanese medicine, is known as Kampo medicine in Japan. We investigated the possible efficacy of Kampo medicine for periodontal disease. In this study, we examined the in vitro effects of orento on the proliferation of the inflammatory cytokines interleukin (IL)-6 and IL-8, the production of type 1 collagen, and the secretion of alkaline phosphatase (ALP) in the human osteosarcoma cell line Saos-2 (Saos-2 cells). Methods: The proliferation of Saos-2 cells was assessed by MTT assay. IL-6 and IL-8 levels, type 1 collagen production and ALP secretion were evaluated using enzyme-linked immunosorbent assay and ALP assays. Saos-2 cells were treated with or without 0.1, 1, 10, 100 and 1000 μg/mL of orento for 24 h. Results: Orento (10 μg/mL) significantly induced the proliferation of Saos-2 cells. At this concentration, orento suppressed IL-6 and IL-8 and enhanced type 1 collagen production and ALP secretion. Conclusions: These results indicate that orento controls the IL-6 and IL-8 secretion and cellular metabolism of osteoblasts, resulting in the secretion of early bone-related biomarkers.

scholarly journals Significance of NF-kappaB signaling and PARP1 activity in the TNF-induced inhibition of PHEX gene expression in human osteoblasts

2018 ◽  
Author(s):  
Paweł Marek Majewski ◽  
Urszula Kędzierska ◽  
Łukasz Banasiak ◽  
Paweł Kiela
Keyword(s):  
Gene Expression ◽  
Cell Line ◽  
Molecular Mechanism ◽  
Osteosarcoma Cell Line ◽  
Proximal Promoter ◽  
Osteosarcoma Cell ◽  
Gene Promoters ◽  
Mineral Density ◽  
Human Osteoblasts ◽  
Phex Gene

Although loss of bone mineral density is a common symptom of chronic inflammatory diseases, its mechanisms are still poorly understood. The PHEX gene encodes a Zn-endopeptidase expressed in osteoblasts and contributes to bone mineralization. Data derived from rodents has indicated co-repression of the PHEX gene by the NF-κB pathway and poly(ADP-ribose) polymerase 1 (PARP1). The aim of this study was to determine the molecular mechanism involved in TNF-mediated downregulation of PHEX expression in human osteoblasts and human osteosarcoma cell line. We observed that activation of the NF-κB pathway by TNF was manifested as a nuclear increase in RELA and NFKB1 heterodimer. We found that TNF reduced PHEX expression and the proteasome inhibitor reversed this effect in osteosarcoma cell line. Contrary to the effects seen in rodents, inhibition of PARP1 enzymatic activity did not significantly reverse the effect of TNF on the human PHEX gene expression. EMSA studies showed that the number of adenines in the PHEX proximal promoter is crucial for the transcription factors’ interactions within that region. The obtained results support the hypothesis indicating the existence of a molecular mechanism of gene repression that involves a poly adenine-rich region of the proximal gene promoters and PARP1 transcriptional activity.

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