Remarkable DNA binding affinity and potential anticancer activity of pyrrolo[2,1-c][1,4]benzodiazepine–naphthalimide conjugates linked through piperazine side-armed alkane spacers

2008 ◽  
Vol 16 (15) ◽  
pp. 7218-7224 ◽  
Author(s):  
Ahmed Kamal ◽  
R. Ramu ◽  
Venkatesh Tekumalla ◽  
G.B. Ramesh Khanna ◽  
Madan S. Barkume ◽  
...  
Keyword(s):  
Dna Binding ◽  
Anticancer Activity ◽  
Binding Affinity ◽  
Dna Binding Affinity

Synthesis, spectroscopic investigation, and DFT study of N,N′-disubstituted ferrocene-based thiourea complexes as potent anticancer agents

2018 ◽  
Vol 47 (6) ◽  
pp. 1868-1878 ◽  
Author(s):  
Faiza Asghar ◽  
Saira Fatima ◽  
Sadaf Rana ◽  
Amin Badshah ◽  
Ian S. Butler ◽  
...  
Keyword(s):  
Dna Binding ◽  
Anticancer Activity ◽  
Binding Affinity ◽  
Anticancer Agents ◽  
Spectroscopic Investigation ◽  
Dft Study ◽  
Redox Potentials ◽  
Dna Binding Affinity ◽  
Dna Binders ◽  
Homo Lumo

The DNA binding affinity of ferrocenyl thioureas (A1–A9) explored by CV and UV ascertain them as noble DNA binders. The complexes also publicized decent antioxidant and anticancer activity. DFT-based HOMO/LUMO energies are comparable with experimentally calculated redox potentials.

Synthesis, DNA binding affinity and anticancer activity of novel 4H-benzo[g][1,2,3]triazolo[5,1-c][1,4]oxazocines

2016 ◽  
Vol 14 (39) ◽  
pp. 9294-9305 ◽  
Author(s):  
K. N. Visweswara Sastry ◽  
Sunitha Rani Routhu ◽  
Soma Gupta Datta ◽  
Narayana Nagesh ◽  
Bathini Nagendra Babu ◽  
...  
Keyword(s):  
Dna Binding ◽  
Anticancer Activity ◽  
Binding Affinity ◽  
Cancer Therapeutics ◽  
New Class ◽  
Dna Binding Affinity

Cancer therapeutics: a new class of anticancer heterocycles was synthesized.

Solid-phase synthesis of new pyrrolobenzodiazepine–chalcone conjugates: DNA-binding affinity and anticancer activity

2008 ◽  
Vol 18 (7) ◽  
pp. 2434-2439 ◽  
Author(s):  
Ahmed Kamal ◽  
N. Shankaraiah ◽  
S. Prabhakar ◽  
Ch. Ratna Reddy ◽  
N. Markandeya ◽  
...  
Keyword(s):  
Dna Binding ◽  
Anticancer Activity ◽  
Binding Affinity ◽  
Solid Phase Synthesis ◽  
Solid Phase ◽  
Phase Synthesis ◽  
Dna Binding Affinity

DNA-binding affinity and anticancer activity of β-carboline–chalcone conjugates as potential DNA intercalators: Molecular modelling and synthesis

2015 ◽  
Vol 59 ◽  
pp. 130-139 ◽  
Author(s):  
Nagula Shankaraiah ◽  
K.P. Siraj ◽  
Shalini Nekkanti ◽  
Vunnam Srinivasulu ◽  
Pankaj Sharma ◽  
...  
Keyword(s):  
Dna Binding ◽  
Anticancer Activity ◽  
Binding Affinity ◽  
Molecular Modelling ◽  
Dna Intercalators ◽  
Dna Binding Affinity

scholarly journals Lowering DNA binding affinity of SssI DNA methyltransferase does not enhance the specificity of targeted DNA methylation in E. coli

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Krystyna Ślaska-Kiss ◽  
Nikolett Zsibrita ◽  
Mihály Koncz ◽  
Pál Albert ◽  
Ákos Csábrádi ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Dna Binding ◽  
Binding Affinity ◽  
Dna Methyltransferase ◽  
Restriction Enzymes ◽  
Dna Binding Protein ◽  
E Coli ◽  
Dna Binding Affinity ◽  
Higher Eukaryotes

AbstractTargeted DNA methylation is a technique that aims to methylate cytosines in selected genomic loci. In the most widely used approach a CG-specific DNA methyltransferase (MTase) is fused to a sequence specific DNA binding protein, which binds in the vicinity of the targeted CG site(s). Although the technique has high potential for studying the role of DNA methylation in higher eukaryotes, its usefulness is hampered by insufficient methylation specificity. One of the approaches proposed to suppress methylation at unwanted sites is to use MTase variants with reduced DNA binding affinity. In this work we investigated how methylation specificity of chimeric MTases containing variants of the CG-specific prokaryotic MTase M.SssI fused to zinc finger or dCas9 targeting domains is influenced by mutations affecting catalytic activity and/or DNA binding affinity of the MTase domain. Specificity of targeted DNA methylation was assayed in E. coli harboring a plasmid with the target site. Digestions of the isolated plasmids with methylation sensitive restriction enzymes revealed that specificity of targeted DNA methylation was dependent on the activity but not on the DNA binding affinity of the MTase. These results have implications for the design of strategies of targeted DNA methylation.

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