scholarly journals Evaluation of in-house mpt64 real-time PCR for rapid detection of Mycobacterium tuberculosis in pulmonary and extra-pulmonary specimens

2012 ◽  
Vol 16 (5) ◽  
pp. 493-494 ◽  
Author(s):  
Sunil Sethi ◽  
Rakesh Yadav ◽  
Abhishek Mewara ◽  
Sunil Kumar Dhatwalia ◽  
Meera Sharma ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Real Time ◽  
Real Time Pcr ◽  
Rapid Detection ◽  
Extra Pulmonary

scholarly journals Locked Nucleic Acid Probe-Based Real-Time PCR Assay for the Rapid Detection of Rifampin-Resistant Mycobacterium tuberculosis

PLoS ONE ◽  
2015 ◽  
Vol 10 (11) ◽  
pp. e0143444 ◽  
Author(s):  
Yong Zhao ◽  
Guilian Li ◽  
Chongyun Sun ◽  
Chao Li ◽  
Xiaochen Wang ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Nucleic Acid ◽  
Real Time ◽  
Real Time Pcr ◽  
Rapid Detection ◽  
Locked Nucleic Acid ◽  
Pcr Assay ◽  
Locked Nucleic Acid Probe

scholarly journals Rapid Detection of Mycobacterium tuberculosis Beijing Genotype Strains by Real-Time PCR

2006 ◽  
Vol 44 (9) ◽  
pp. 3472-3472
Author(s):  
D. Hillemann ◽  
R. Warren ◽  
T. Kubica ◽  
S. Rusch-Gerdes ◽  
S. Niemann
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Real Time ◽  
Real Time Pcr ◽  
Rapid Detection ◽  
Beijing Genotype

Improved real-time PCR for rapid detection of rifampin and isoniazid resistance in Mycobacterium tuberculosis clinical isolates

2003 ◽  
Vol 45 (3) ◽  
pp. 207-212 ◽  
Author(s):  
Maria J Torres ◽  
Antonio Criado ◽  
Maite Ruiz ◽  
Ana C Llanos ◽  
Jose C Palomares ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Real Time ◽  
Real Time Pcr ◽  
Rapid Detection ◽  
Clinical Isolates ◽  
Isoniazid Resistance

scholarly journals Rapid Detection of Mycobacterium tuberculosis Beijing Genotype Strains by Real-Time PCR

2006 ◽  
Vol 44 (2) ◽  
pp. 302-306 ◽  
Author(s):  
D. Hillemann ◽  
R. Warren ◽  
T. Kubica ◽  
S. Rusch-Gerdes ◽  
S. Niemann
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Real Time ◽  
Real Time Pcr ◽  
Rapid Detection ◽  
Beijing Genotype

scholarly journals Rapid Direct Detection of Multiple Rifampin and Isoniazid Resistance Mutations in Mycobacterium tuberculosis in Respiratory Samples by Real-Time PCR

2004 ◽  
Vol 48 (11) ◽  
pp. 4293-4300 ◽  
Author(s):  
Mercedes Marín ◽  
Darío García de Viedma ◽  
María Jesús Ruíz-Serrano ◽  
Emilio Bouza
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Real Time ◽  
Real Time Pcr ◽  
Rapid Detection ◽  
Direct Detection ◽  
Bacterial Load ◽  
Clinical Samples ◽  
Sequencing Data ◽  
Resistance Mutations ◽  
Time Required

ABSTRACT Rapid detection of resistance in Mycobacterium tuberculosis can optimize the efficacy of antituberculous therapy and control the transmission of resistant M. tuberculosis strains. Real-time PCR has minimized the time required to obtain the susceptibility pattern of M. tuberculosis strains, but little effort has been made to adapt this rapid technique to the direct detection of resistance from clinical samples. In this study, we adapted and evaluated a real-time PCR design for direct detection of resistance mutations in clinical respiratory samples. The real-time PCR was evaluated with (i) 11 clinical respiratory samples harboring bacilli resistant to isoniazid (INH) and/or rifampin (RIF), (ii) 10 culture-negative sputa spiked with a set of strains encoding 14 different resistance mutations in 10 independent codons, and (iii) 16 sputa harboring susceptible strains. The results obtained with this real-time PCR design completely agreed with DNA sequencing data. In all sputa harboring resistant M. tuberculosis strains, the mutation encoding resistance was successfully detected. No mutation was detected in any of the susceptible sputa. The test was applied only to smear-positive specimens and succeeded in detecting a bacterial load equivalent to 103 CFU/ml in sputum samples (10 acid-fast bacilli/line). The analytical specificity of this method was proved with a set of 14 different non-M. tuberculosis bacteria. This real-time PCR design is an adequate method for the specific and rapid detection of RIF and INH resistance in smear-positive clinical respiratory samples.

Sign in / Sign up

Export Citation Format

Share Document