Production of a ruminal bacterial phytase in the green microalga Chlamydomonas reinhardtii with potential applications in monogastric animal feed

2021 ◽  
Vol 14 ◽  
pp. 100660
Author(s):  
Santy Peraza-Echeverria ◽  
Shirley Bernardo-Candelero ◽  
Fray Martin Baas-Espinola ◽  
Carlos Puch-Hau ◽  
Rodrigo Arturo Rivera-Solís ◽  
...  
Keyword(s):  
Chlamydomonas Reinhardtii ◽  
Animal Feed ◽  
Monogastric Animal ◽  
Green Microalga ◽  
Potential Applications

scholarly journals The Potential Utilization of High-Fiber Agricultural By-Products as Monogastric Animal Feed and Feed Additives: A Review

Animals ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 2098
Author(s):  
Wen-Yang Chuang ◽  
Li-Jen Lin ◽  
Hsin-Der Shih ◽  
Yih-Min Shy ◽  
Shang-Chang Chang ◽  
...  
Keyword(s):  
Dietary Fiber ◽  
Animal Feed ◽  
Animal Health ◽  
Feed Additives ◽  
Health It ◽  
High Fiber ◽  
Monogastric Animal ◽  
Animal Growth ◽  
Positive Effect ◽  
By Products

With the increase in world food demand, the output of agricultural by-products has also increased. Agricultural by-products not only contain more than 50% dietary fiber but are also rich in functional metabolites such as polyphenol (including flavonoids), that can promote animal health. The utilization of dietary fibers is closely related to their types and characteristics. Contrary to the traditional cognition that dietary fiber reduces animal growth, it can promote animal growth and maintain intestinal health, and even improve meat quality when added in moderate amounts. In addition, pre-fermenting fiber with probiotics or enzymes in a controlled environment can increase dietary fiber availability. Although the use of fiber has a positive effect on animal health, it is still necessary to pay attention to mycotoxin contamination. In summary, this report collates the fiber characteristics of agricultural by-products and their effects on animal health and evaluates the utilization value of agricultural by-products.

Anaerobic treatment of residual lemon pulp in digesters with semi-continuous feed

2013 ◽  
Vol 67 (3) ◽  
pp. 514-520
Author(s):  
A. R. Navarro ◽  
Z. Lopez ◽  
J. Salguero ◽  
M. C. Maldonado
Keyword(s):  
Industrial Waste ◽  
Animal Feed ◽  
Oxygen Demand ◽  
Anaerobic Treatment ◽  
Raw Material ◽  
Comparative Tests ◽  
Economic Problems ◽  
The North ◽  
Potential Applications ◽  
Continuous Feed

Lemon growing areas in the north of Argentina have industries that produce concentrated juice, peel and essential oil and generate a significant amount of liquid and solid waste as lemon pulp. In Argentina, despite the potential applications that the pulp has as animal feed and human and industrial raw material, only 10% is used for these purposes and the rest is discarded into the environment causing many ecological and economic problems. There is little information in the literature on biotechnologies for the treatment of this industrial waste. This paper shows that lemon pulp is a suitable substrate to be treated by anaerobic digestion. We obtained 86 and 92% reduction of chemical oxygen demand in a digester with a semi-continuous feed and retention time of 10 and 20 days respectively and a productivity of 0.406 g CH4/g VS h. Comparative tests showed that pre-digesting the pulp improved the process of digestion and increased biogas generation by 20%.

scholarly journals Applications of Microbial β-Mannanases

2020 ◽  
Vol 8 ◽  
Author(s):  
Aneesa Dawood ◽  
Kesen Ma
Keyword(s):  
Catalytic Properties ◽  
Animal Feed ◽  
Plant Tissues ◽  
Degrading Enzymes ◽  
Wide Range ◽  
Potential Applications ◽  
Paper And Pulp ◽  
Main Components ◽  
Temperature Applications

Mannans are main components of hemicellulosic fraction of softwoods and they are present widely in plant tissues. β-mannanases are the major mannan-degrading enzymes and are produced by different plants, animals, actinomycetes, fungi, and bacteria. These enzymes can function under conditions of wide range of pH and temperature. Applications of β-mannanases have therefore, been found in different industries such as animal feed, food, biorefinery, textile, detergent, and paper and pulp. This review summarizes the most recent studies reported on potential applications of β-mannanases and bioengineering of β-mannanases to modify and optimize their key catalytic properties to cater to growing demands of commercial sectors.

scholarly journals Introns mediate post-transcriptional enhancement of nuclear gene expression in the green microalga Chlamydomonas reinhardtii

PLoS Genetics ◽  
2020 ◽  
Vol 16 (7) ◽  
pp. e1008944 ◽  
Author(s):  
Thomas Baier ◽  
Nick Jacobebbinghaus ◽  
Alexander Einhaus ◽  
Kyle J. Lauersen ◽  
Olaf Kruse
Keyword(s):  
Gene Expression ◽  
Chlamydomonas Reinhardtii ◽  
Nuclear Gene ◽  
Nuclear Gene Expression ◽  
Green Microalga

Functional analysis of three type-2 DGAT homologue genes for triacylglycerol production in the green microalga Chlamydomonas reinhardtii

2012 ◽  
Vol 162 (1) ◽  
pp. 13-20 ◽  
Author(s):  
M. La Russa ◽  
C. Bogen ◽  
A. Uhmeyer ◽  
A. Doebbe ◽  
E. Filippone ◽  
...  
Keyword(s):  
Functional Analysis ◽  
Chlamydomonas Reinhardtii ◽  
Green Microalga

scholarly journals Orfamide-A-mediated bacterial-algal interactions involve specific Ca2+ signalling pathways

2021 ◽  
Author(s):  
Yu Hou ◽  
Yuko Bando ◽  
David Carrasco Flores ◽  
Vivien Susann Hotter ◽  
Bastian Schiweck ◽  
...  
Keyword(s):  
Chlamydomonas Reinhardtii ◽  
Trp Channels ◽  
Signalling Pathways ◽  
Antagonistic Bacterium ◽  
Cyclic Lipopeptide ◽  
Green Microalga

The antagonistic bacterium Pseudomonas protegens secretes the cyclic lipopeptide orfamide A, which triggers a Ca2+ signal, causing the deflagellation of the green microalga Chlamydomonas reinhardtii. By investigating targeted synthetic orfamide A variants and inhibitors, we found that at least two Ca2+-signalling pathways and TRP channels are involved in this response.

scholarly journals The Path to Triacylglyceride Obesity in the sta6 Strain of Chlamydomonas reinhardtii

2014 ◽  
Vol 13 (5) ◽  
pp. 591-613 ◽  
Author(s):  
Ursula Goodenough ◽  
Ian Blaby ◽  
David Casero ◽  
Sean D. Gallaher ◽  
Carrie Goodson ◽  
...  
Keyword(s):  
Chlamydomonas Reinhardtii ◽  
Growth Conditions ◽  
Culture Conditions ◽  
Pentose Phosphate ◽  
Small Subset ◽  
Content Type ◽  
Green Microalga ◽  
Genes Encoding ◽  
Encoding Genes ◽  
Tag Accumulation

ABSTRACT When the sta6 (starch-null) strain of the green microalga Chlamydomonas reinhardtii is nitrogen starved in acetate and then “boosted” after 2 days with additional acetate, the cells become “obese” after 8 days, with triacylglyceride (TAG)-filled lipid bodies filling their cytoplasm and chloroplasts. To assess the transcriptional correlates of this response, the sta6 strain and the starch-forming cw15 strain were subjected to RNA-Seq analysis during the 2 days prior and 2 days after the boost, and the data were compared with published reports using other strains and growth conditions. During the 2 h after the boost, ∼425 genes are upregulated ≥2-fold and ∼875 genes are downregulated ≥2-fold in each strain. Expression of a small subset of “sensitive” genes, encoding enzymes involved in the glyoxylate and Calvin-Benson cycles, gluconeogenesis, and the pentose phosphate pathway, is responsive to culture conditions and genetic background as well as to boosting. Four genes—encoding a diacylglycerol acyltransferase ( DGTT2 ), a glycerol-3-P dehydrogenase ( GPD3 ), and two candidate lipases (Cre03.g155250 and Cre17.g735600)—are selectively upregulated in the sta6 strain. Although the bulk rate of acetate depletion from the medium is not boost enhanced, three candidate acetate permease-encoding genes in the GPR1/FUN34/YaaH superfamily are boost upregulated, and 13 of the “sensitive” genes are strongly responsive to the cell's acetate status. A cohort of 64 autophagy-related genes is downregulated by the boost. Our results indicate that the boost serves both to avert an autophagy program and to prolong the operation of key pathways that shuttle carbon from acetate into storage lipid, the combined outcome being enhanced TAG accumulation, notably in the sta6 strain.

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