Nutritional impact of low-cost substrates on biphasic fermentation for conidia production of the fungal biopesticide Metarhizium anisopliae

2021 ◽  
Vol 13 ◽  
pp. 100619
Author(s):  
Polyane de Sá Santos ◽  
Kauana Abati ◽  
Natalie Verónika Rondinel Mendoza ◽  
Gabriel Moura Mascarin ◽  
Italo Delalibera Júnior
Keyword(s):  
Metarhizium Anisopliae ◽  
Low Cost ◽  
Conidia Production ◽  
Biphasic Fermentation

Effects of double-stranded RNA inMetarhizium anisopliaevar.acridumandPaecilomyces fumosoroseuson protease activities, conidia production, and virulence

2004 ◽  
Vol 50 (5) ◽  
pp. 335-339 ◽  
Author(s):  
Patricia Vieira Tiago ◽  
Maria Helena Pelegrinelli Fungaro ◽  
Marcos Rodrigues de Faria ◽  
Marcia Cristina Furlaneto
Keyword(s):  
Metarhizium Anisopliae ◽  
Protease Production ◽  
Secreted Protein ◽  
Paecilomyces Fumosoroseus ◽  
Chromogenic Substrate ◽  
Entomogenous Fungi ◽  
Double Stranded Rna ◽  
Conidia Production ◽  
Isogenic Strains ◽  
Metarhizium Anisopliae Var Acridum

Isogenic strains (with and without dsRNA) of the entomogenous fungi Metarhizium anisopliae var. acridum and Paecilomyces fumosoroseus were investigated for correlation between the presence of dsRNA and the production of cuticle-degrading proteases that play an important role in host parasitism, total secreted protein, and conidia production. Similar levels of cuticle-degrading subtilisin-like (Pr1) protease were observed for isogenic strains of M. anisopliae var. acridum after growth in medium supplemented with the cuticle of the grasshopper Rhammatocerus schistocercoides. Similarly, no statistical differences were observed for protease production, detected using the chromogenic substrate azocasein. For P. fumosoroseus isogenic strains, no significant differences in protease activity were observed after growth in the presence of either Euschistus heros or Nezara viridula (Hemiptera: Pentatomidae) cuticle. Similarly, no statistical differences were observed in virulence against E. heros. A comparison of mean conidia production showed a significantly higher production in the dsRNA-free isogenic strains of M. anisopliae var. acridum. Although, for most of the fungal phenotypes analysed, no overt effects were associated with the presence of these dsRNA infections, the reduction in conidia production by the isogenic strains of M. anisopliae var. acridum with dsRNA suggested that it may not be entirely accurate to describe these infections as latent.Key words: Metarhizium anisopliae var. acridum, Paecilomyces fumosoroseus, double-stranded RNA (dsRNA), proteases, conidia production, virulence.

Effects of low temperature on growth parameters in the entomopathogenic fungus Metarhizium anisopliae

1999 ◽  
Vol 45 (12) ◽  
pp. 1055-1061 ◽  
Author(s):  
J N Amritha De Croos ◽  
Michael J Bidochka
Keyword(s):  
Growth Rate ◽  
Growth Rates ◽  
Metarhizium Anisopliae ◽  
Entomopathogenic Fungus ◽  
Incubation Temperature ◽  
Growth Parameters ◽  
Conidia Production ◽  
Cold Active ◽  
Germination And Growth ◽  
Cold Activity

The effects of temperature (8°C, 15°C, and 22°C) on germination, growth rate and conidia production in thirty-two isolates of the entomopathogenic fungus Metarhizium anisopliae were assessed. Most isolates were obtained from various locations in Ontario, Canada. Ten out of thirty-two (31.3%) isolates were deemed cold-active because of their ability to grow at 8°C. Growth rates in all isolates increased as the incubation temperature was increased. There were significant differences in growth rates and conidia production among isolates. However, conidia production had no relationship with a growth rate. With respect to the geographical origin, there was no general relationship between latitude and growth rates. However, we found that all the cold-active isolates were isolated from the more northern sites and no isolate originating below 43.5° latitude showed cold activity. Cold-active germination and growth of this biocontrol fungus have implications for strain selection and application in Canadian insect control efforts.Key words: Metarhizium anisopliae, cold activity, entomopathogen.

scholarly journals COMPATIBILITY OF NEUTRAL DETERGENT WITH THE ENTOMOPATHOGENIC FUNGUS METARHIZIUM ANISOPLIAE (METSCH.) SOROK.

2011 ◽  
Vol 78 (3) ◽  
pp. 471-474
Author(s):  
E.S. Harterreiten-Souza ◽  
L.G.A. Pessoa ◽  
E. de S. Loureiro
Keyword(s):  
Entomopathogenic Fungi ◽  
Metarhizium Anisopliae ◽  
Vegetative Growth ◽  
Entomopathogenic Fungus ◽  
Tween 80 ◽  
Genetic Composition ◽  
Fungus Species ◽  
Hydrophobic Character ◽  
Colony Forming Units ◽  
Conidia Production

ABSTRACT Entomopathogenic fungi have been widely used in pests control. The main diluent for its application in the field is water, and due to its hydrophobic character, its dispersion in the environment is complicated. Several products may be mixed to the sprays, allowing the dissolution and the dispersion of the fungus in the spray. Yet, some of these products may influence in the viability, sporulation or even in its genetic composition, changing its virulence. This paper aimed to evaluate the effect of a neutral detergent about the viability of the entomopathogenic fungus Metarhizium anisopliae. Three different concentrations of a neutral detergent (Ipê®) were used: 0.01%, 0.02%, 0.03% and an adhesive spreader (Tween 80) was used in the control. The percentage of conidia germination, the number of colony forming units (CFU), vegetative growth and the number of produced conidia were evaluated. There was no effect of the different concentrations of the neutral detergent on the germination. All detergent concentrations affected negatively in the vegetative growth of the fungus. Only the detergent at 0.01% of concentration did not affect the CFU number and the conidia production of the fungus. So, this is the only concentration classified as compatible for M. anisopliae, which may be recommended to promote the conidia dispersion of this fungus species in water.

Conidia production of Metarhizium anisopliae in bags and packed column bioreactors

2018 ◽  
Vol 7 (1) ◽  
pp. 65-69 ◽  
Author(s):  
Fernando Mendez-Gonzalez ◽  
Octavio Loera ◽  
Ernesto Favela-Torres
Keyword(s):  
Metarhizium Anisopliae ◽  
Packed Column ◽  
Conidia Production

scholarly journals Evaluation and Optimization of Agro-industrial Wastes for Conidial Production of Metarhizium anisopliae isolates under Solid State Fermentation

1970 ◽  
Vol 11 (2) ◽  
pp. 209-228
Author(s):  
Tewelde Abiy ◽  
Alemu Tesfaye
Keyword(s):  
Solid State ◽  
Wheat Bran ◽  
Solid State Fermentation ◽  
Metarhizium Anisopliae ◽  
Large Scale ◽  
Agricultural Wastes ◽  
Coffee Husk ◽  
Tea Waste ◽  
Conidia Production ◽  
Vegetable Wastes

Metarhizium anisopliae is known to cause high level of epizootics for more than 200 insect species in versatile agro-ecologies. Concerns on environmental pollution and resistance development to chemical insecticides need environmentally safe and economically viable approaches. Therefore, here we investigate a cheap and large scale industrial production of virulent enthomopathogenes on agricultural wastes. Three Metarhizium anisopliae isolates were grown on agricultural wastes to evaluate their conidia production potential under Solid state fermentation (SSF) technique. Coffee husk, tea waste, wheat bran and vegetable wastes were used as substrates to determine their capability for maximum conidiation of the isolates. Among these, vegetable wastes were the best media to yield 5.80 ±0.72 (107), 4.44±0.55 (107) and 5.58±0.66 (107) conidia/gram of substrate under quantitative assessment for isolate AUMI1, AUMI2 and AUMI3 respectively, at 60% moisture content.  Statistically on two sample t-test vegetable wastes shows significant difference in conidia production when compared to 2 mm and 4 mm sized coffee husk used as substrates. The optimization for temperature indicated that all substrates supported their maximum conidia yield within 27 – 300C range of temperature. The 3.5 pH value used in the present study for optimization was best favored only for coffee husk as substrate. The high conidia yielding substrates were best productive at pH 6.29, 6.63 and 5.4 for vegetable wastes, wheat bran and tea waste, respectively. All isolates incubated on wheat bran was highly productive under sufficient exposure to light. AUMI1 produced high conidia under exposure to light while the higher yield of AUMI2 and AUMI3 was produced under dark condition on vegetable wastes. Therefore, as successful microbial control of insect pests depends on large scale and cheap industrial productivity, cultivation on vegetable wastes and wheat bran under SSF can be a plausible solution.

scholarly journals Alternative substrates for conidiogenesis of the entomopathogenic fungus Beauveria bassiana (Bals) Vuillemin (Deuteromycotina: Hyphomycetes)

2020 ◽  
Vol 80 (1) ◽  
pp. 133-141
Author(s):  
A. G. L. Paiva-Guimarães ◽  
K. R. L. Freire ◽  
S. F. M. Santos ◽  
A. F. Almeida ◽  
A.C.B. Sousa
Keyword(s):  
Biological Control ◽  
Beauveria Bassiana ◽  
Entomopathogenic Fungi ◽  
Insecticidal Activity ◽  
Insect Pests ◽  
Low Cost ◽  
Nutritional Supplement ◽  
Conidia Production ◽  
Fungus Beauveria Bassiana ◽  
Potential Use

Abstract Beauveria bassiana is a promising fungus for the biological control of insect pests. The growing costs of conidia production have raised the need to ascertain the efficiency of some low cost substrates. The aim of this study was to analyze the potential use of different raw substrates without nutritional supplement for B. bassiana conidiogenesis. Growth and sporulation were evaluated using 30 g of substrate and 0.3 μL of a conidia suspension (1 x 106 conidia/mL). After 10 days of incubation (70 ± 10% humidity and temperature (T) = 29 ± 1 °C), rice (2.00 x 106 conidia/g substrate), algaroba (2.36 x 106 conidia/g), malt A (1.22 x 106 conidia/g) and malt B (1.75 x 106 conidia/g) showed the highest levels of conidia production. The resulting conidia showed insecticidal activity higher than 80% on coconut termites. These new raw substrates may represent viable alternatives for the production of entomopathogenic fungi for use in the biological control of various insect pests.

scholarly journals Metarhizium anisopliae conidia production in packed-bed bioreactor using rice as substrate in successive cultivations

2020 ◽  
Vol 97 ◽  
pp. 104-111 ◽  
Author(s):  
Lucas Portilho da Cunha ◽  
Fernanda Perpétua Casciatori ◽  
Isabela Valente Vicente ◽  
Renata Laurito Garcia ◽  
João Cláudio Thoméo
Keyword(s):  
Metarhizium Anisopliae ◽  
Packed Bed ◽  
Packed Bed Bioreactor ◽  
Conidia Production
Sign in / Sign up

Export Citation Format

Share Document