Butyric acid as sole product from xylose fermentation by a non-solventogenic Clostridium beijerinckii strain under controlled pH and nutritional conditions

2020 ◽  
Vol 10 ◽  
pp. 100426
Author(s):  
Bruna Constante Fonseca ◽  
Jonatã Bortolucci ◽  
Thalita Marques da Silva ◽  
Vinícius Fabiano dos Passos ◽  
Paula Fagundes de Gouvêa ◽  
...  
Keyword(s):  
Butyric Acid ◽  
Xylose Fermentation ◽  
Clostridium Beijerinckii ◽  
Nutritional Conditions ◽  
Sole Product

scholarly journals Production of butyric acid at constant pH by a solventogenic strain of Clostridium beijerinckii

2020 ◽  
Vol 38 (No. 3) ◽  
pp. 185-191 ◽  
Author(s):  
Marek Drahokoupil ◽  
Petra Patáková
Keyword(s):  
Flow Cytometry ◽  
Butyric Acid ◽  
Population Viability ◽  
Clostridium Beijerinckii ◽  
Clostridium Tyrobutyricum ◽  
Fluorescence Staining ◽  
Low Concentration ◽  
Ph Values ◽  
Constant Ph ◽  
Acetone Butanol Ethanol

A solventogenic strain of Clostridium beijerinckii, NRRL B-598, was cultured for the production of butyric acid as the main fermentation product. However, unlike typical acetone-butanol-ethanol (ABE) fermentations, where pH is not regulated, in this study the pH was kept constant during fermentation. From the five pH values tested, 6.0, 6.5, 7.0, 7.5 and 8.0, pH 6.5 and 7.0 resulted in the highest concentrations of butyric acid, at 9.69 ± 0.09 g L–1 and 11.5 ± 0.39 g L–1, respectively. However, a low concentration of solvents, 1.8 ± 0.22 g L–1, was only reached at pH 7.0. These results are comparable with those from typical butyric acid producers, i.e. Clostridium butyricum and Clostridium tyrobutyricum strains. At pH 7.0, we succeeded in suppressing sporulation and prolonging the population viability, which was confirmed by flow cytometry combined with double fluorescence staining.

scholarly journals 1,3-PROPANEDIOL: STATISTICAL OPTIMIZATION OF MEDIUM TO IMPROVE PRODUCTION BY Clostridium beijerinckii DSM 791

2015 ◽  
Vol 5 (2) ◽  
pp. 614-624 ◽  
Author(s):  
Daiana Wischral
Keyword(s):  
Experimental Design ◽  
Butyric Acid ◽  
Yeast Extract ◽  
Desirability Function ◽  
Fermentation Medium ◽  
Clostridium Beijerinckii ◽  
Fractional Factorial ◽  
Promising Alternative ◽  
Statistical Effect ◽  
Glycerol Consumption

Biodiesel is a promising alternative biofuel, and as its production increases, so does production of the principal co-product, glycerol. The present study is aimed at maximizing the glycerol consumption and 1,3-propanediol (1,3-PDO) production by C. beijerinckii DSM 791, using sequential experimental design methodology for optimization of nutrients concentration for the fermentation medium. Three sequential experimental designs were performed: Plackett-Burman, fractional factorial and central composite rotational. The following nutrients of the medium were evaluated: urea, yeast extract, C2H3NaO2, KH2PO4, K2HPO4, MgSO4.7H2O, MnSO4.H2O, FeSO4.7H2O, glycerol, butyric acid, glucose and CaCO3. Urea, KH2PO4, MgSO4.7H2O, MnSO4.H2O, FeSO4.7H2O, butyric acid, glucose and CaCO3 were removed from the medium optimization since they had an insignificant statistical effect on 1,3-PDO production. The optimal concentrations of yeast extract, C2H3NaO2, K2HPO4 and glycerol predicted by the optimization were as follows (g/l): 0.5, 0.005, 5 and 8, respectively, which were validated experimentally. Desirability function allows the maximization of the 1,3-PDO production and percentage glycerol consumption simultaneously, which resulted in yield of 0.58 mol/mol of 1,3-PDO and 100 % of glycerol consumption. The results showed that the use of sequential experimental design and the use of the desirability function led to the optimization of the 1,3-PDO fermentation by C. beijerinckii DSM 791.

The pH-stat Butyric Acid Feeding Strategy Coupled with Gas-Stripping for n-Butanol Production by Clostridium beijerinckii

2018 ◽  
Vol 11 (3) ◽  
pp. 1077-1084
Author(s):  
Doo-Geun Lee ◽  
Jong-Min Jeon ◽  
Yung-Hun Yang ◽  
Yong-Su Jin ◽  
Jeong-Jun Yoon
Keyword(s):  
Butyric Acid ◽  
Feeding Strategy ◽  
Clostridium Beijerinckii ◽  
Butanol Production ◽  
Gas Stripping ◽  
Ph Stat

A novel isolate of Clostridium butyricum for efficient butyric acid production by xylose fermentation

2018 ◽  
Vol 68 (6) ◽  
pp. 321-330
Author(s):  
Xin Wang ◽  
Jianzheng Li ◽  
Xue Chi ◽  
Yafei Zhang ◽  
Han Yan ◽  
...  
Keyword(s):  
Butyric Acid ◽  
Acid Production ◽  
Xylose Fermentation ◽  
Clostridium Butyricum ◽  
Butyric Acid Production

Nickel(0)-Catalyzed Linear-Selective Hydroarylation of Unactivated Alkenes and Styrenes with Arylboron Acids

2018 ◽  
Author(s):  
Honggui Lv ◽  
Li-Jun Xiao ◽  
Dongbing Zhao ◽  
Qi-Lin Zhou
Keyword(s):  
Butyric Acid ◽  
Biologically Active Compounds ◽  
Biologically Active ◽  
Active Compounds ◽  
Highly Efficient ◽  
Organoboronic Acids

Herein, we realized the first linear-selective hydroarylation of unactivated alkenes and styrenes with organoboronic acids by introducing directing groupon alkenes. Our method is highly efficient and scalable, and provides a modular route to assemble structurally diverse alkylarenes, especially for γ-aryl butyric acid derivatives, which have been widely utilized as chemical feedstocks to access multiple marketed drugs, and biologically active compounds.<br>

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