Evaluation of the potential of an enzymatically treated beech wood hydrolysate as carbon source for Sulfolobus acidocaldarius

2020 ◽  
Vol 9 ◽  
pp. 100362
Author(s):  
Julian Quehenberger ◽  
Susanne Steudler ◽  
Oliver Spadiut
Keyword(s):  
Carbon Source ◽  
Beech Wood ◽  
Sulfolobus Acidocaldarius ◽  
Wood Hydrolysate

scholarly journals Evaluating the Potential of Rhodosporidium toruloides-1588 for High Lipid Production Using Undetoxified Wood Hydrolysate as a Carbon Source

Energies ◽  
2020 ◽  
Vol 13 (22) ◽  
pp. 5960
Author(s):  
Rahul Saini ◽  
Krishnamoorthy Hegde ◽  
Carlos Saul Osorio-Gonzalez ◽  
Satinder Kaur Brar ◽  
Pierre Vezina
Keyword(s):  
Carbon Source ◽  
Low Cost ◽  
Lipid Production ◽  
Rhodosporidium Toruloides ◽  
Biofuel Production ◽  
Sugar Consumption ◽  
High Lipid ◽  
Lipid Characterization ◽  
Forestry Residues ◽  
Wood Hydrolysate

The study aims to explore microbial lipid production using an abundant and low-cost lignocellulosic biomass derived from forestry residues. Sugar-rich undetoxified hydrolysate was prepared using hardwood and softwood sawdust and used for lipid production as a carbon source from an oleaginous yeast, Rhodosporidium toruloides-1588. The maximum biomass obtained was 17.09 and 19.56 g/L in hardwood and softwood hydrolysate, respectively. Sugar consumption in both hydrolysates was >95%, with a maximum lipid accumulation of 36.68% at 104 h and 35.24% at 96 h. Moreover, R. toruloides-1588 exhibited tolerance to several toxic compounds such as phenols, organic acids and furans present in hydrolysates. The lipid characterization showed several monosaturated and polyunsaturated fatty acids, making it a potential feedstock for biofuels and oleochemicals production. This study confirms the credibility of R. toruloides-1588 as a suitable lipid producer using hydrolysates from forestry residues as a substrate. Additionally, lipids obtained from R. toruloides-1588 could be a potential feedstock for advanced biofuel production as well as for food and pharmaceutical applications.

scholarly journals The Impact of Pyroglutamate:Sulfolobus acidocaldariusHas a Growth Advantage overSaccharolobus solfataricusin Glutamate-Containing Media

Archaea ◽  
2019 ◽  
Vol 2019 ◽  
pp. 1-9
Author(s):  
Anna M. Vetter ◽  
Julia Helmecke ◽  
Dietmar Schomburg ◽  
Meina Neumann-Schaal
Keyword(s):  
Carbon Source ◽  
Sole Carbon Source ◽  
Sulfolobus Solfataricus ◽  
Cell Extract ◽  
Sulfolobus Acidocaldarius ◽  
Intracellular Accumulation ◽  
Efficient Utilization ◽  
Crude Cell Extract ◽  
Toxic Metabolites ◽  
The Impact

Microorganisms are well adapted to their habitat but are partially sensitive to toxic metabolites or abiotic compounds secreted by other organisms or chemically formed under the respective environmental conditions. Thermoacidophiles are challenged by pyroglutamate, a lactam that is spontaneously formed by cyclization of glutamate under aerobic thermoacidophilic conditions. It is known that growth of the thermoacidophilic crenarchaeonSaccharolobus solfataricus(formerlySulfolobus solfataricus) is completely inhibited by pyroglutamate. In the present study, we investigated the effect of pyroglutamate on the growth ofS. solfataricusand the closely related crenarchaeonSulfolobus acidocaldarius.In contrast toS. solfataricus,S. acidocaldariuswas successfully cultivated with pyroglutamate as a sole carbon source. Bioinformatical analyses showed that both members of theSulfolobaceaehave at least one candidate for a 5-oxoprolinase, which catalyses the ATP-dependent conversion of pyroglutamate to glutamate. InS. solfataricus, we observed the intracellular accumulation of pyroglutamate and crude cell extract assays showed a less effective degradation of pyroglutamate. Apparently,S. acidocaldariusseems to be less versatile regarding carbohydrates and prefers peptidolytic growth compared toS. solfataricus. Concludingly,S. acidocaldariusexhibits a more efficient utilization of pyroglutamate and is not inhibited by this compound, making it a better candidate for applications with glutamate-containing media at high temperatures.

Morphological Characterization of Mycobacteriophage R1

1974 ◽  
Vol 32 ◽  
pp. 254-255
Author(s):  
B. L. Soloff ◽  
T. A. Rado
Keyword(s):  
Carbon Source ◽  
Stationary Phase ◽  
Growth Phase ◽  
Minimal Medium ◽  
Morphological Characterization ◽  
Logarithmic Growth Phase ◽  
Complete Lysis ◽  
Lysogenic Culture ◽  
Logarithmic Growth

Mycobacteriophage R1 was originally isolated from a lysogenic culture of M. butyricum. The virus was propagated on a leucine-requiring derivative of M. smegmatis, 607 leu−, isolated by nitrosoguanidine mutagenesis of typestrain ATCC 607. Growth was accomplished in a minimal medium containing glycerol and glucose as carbon source and enriched by the addition of 80 μg/ ml L-leucine. Bacteria in early logarithmic growth phase were infected with virus at a multiplicity of 5, and incubated with aeration for 8 hours. The partially lysed suspension was diluted 1:10 in growth medium and incubated for a further 8 hours. This permitted stationary phase cells to re-enter logarithmic growth and resulted in complete lysis of the culture.

Enantioselective Multi-Component Cyclopropane Synthesis Enabled by Cu-Catalyzed Cyclopropene Carbometallation with Organoboron Reagent

2018 ◽  
Author(s):  
Zhanyu Li ◽  
Mengru Zhang ◽  
Yu Zhang ◽  
Shuang Liu ◽  
Jinbo Zhao ◽  
...  
Keyword(s):  
Carbon Source ◽  
Stereoselective Synthesis ◽  
Organometallic Reagents ◽  
Preliminary Results ◽  
Quaternary Center ◽  
Chiral Centers ◽  
Substituted Cyclopropanes ◽  
Proof Of Principle

Deployment of organoboron in lieu of the strongly basic <br>organometallic reagents as carbon source in Cu-catalyzed <br>cyclopropene carbometallation opens unprecedented three-<br>component reactivity for stereoselective synthesis of poly-substituted cyclopropanes. A proof-of-principle demonstration of this novel carbometallation strategy is presented herein for a highly convergent access to poly-substituted aminocyclopropane framework via <br>carboamination. Preliminary results on asymmetric desymmetrization with commercial bisphosphine ligands attained high levels of enantioselection, offering a straightforward access to enantioenriched aminocyclopropanes bearing all-three chiral centers, including an all-carbon quaternary center. This strategy may underpin a host of novel synthetic protocols for poly-substituted cyclopropanes. <br>

Über die mechanische Bedeutung der Holzstrahlen | The mechanical relevance of wood rays

2003 ◽  
Vol 154 (12) ◽  
pp. 498-503 ◽  
Author(s):  
Ingo Burgert
Keyword(s):  
Beech Wood ◽  
Tensile Tests ◽  
Deciduous Tree ◽  
Biological Design ◽  
Transverse Tensile ◽  
Radial Reinforcement ◽  
Radial Strength ◽  
Transverse Tensile Strength ◽  
Strength And Stiffness ◽  
Technical Solutions

Three investigations into the mechanical relevance of wood rays were combined for this article. The main objective was to show, that, apart from physiological functions, rays also significantly influence the radial strength and stiffness of wood. In the first approach twelve deciduous tree species with various proportions of fractions of rays were examined for their transverse tensile strength and stiffness. The second approach was based on the comparison of the radial mechanical properties of wood with a very high proportion of fraction of rays and beech wood with a normal volume. In these two investigations the mechanical relevance of rays could only be deduced indirectly. By isolating big rays of beech and carrying out tensile tests on the tissue, we found direct evidence for the mechanical relevance. The results are discussed with regard to their biomechanical relevance. The importance of a radial reinforcement for the wood is underlined. Moreover, the principle of multi-functionality in nature is emphasized in keeping with a possible transfer of biological design to technical solutions.

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