Ultrasensitive Faraday cage-type electrochemiluminescence assay for femtomolar miRNA-141 via graphene oxide and hybridization chain reaction-assisted cascade amplification

2018 ◽  
Vol 109 ◽  
pp. 13-19 ◽  
Author(s):  
Jing Lu ◽  
Lin Wu ◽  
Yufang Hu ◽  
Sui Wang ◽  
Zhiyong Guo
Keyword(s):  
Graphene Oxide ◽  
Hybridization Chain Reaction ◽  
Chain Reaction ◽  
Faraday Cage ◽  
Cascade Amplification

scholarly journals Sensitive DNA detection using cascade amplification strategy based on conjugated polyelectrolytes and hybridization chain reaction

RSC Advances ◽  
2017 ◽  
Vol 7 (6) ◽  
pp. 3528-3533 ◽  
Author(s):  
Biqing Bao ◽  
Jin Zhu ◽  
Lina Gong ◽  
Jia Chen ◽  
Yanrui Pan ◽  
...  
Keyword(s):  
Signal Amplification ◽  
Dna Detection ◽  
Hybridization Chain Reaction ◽  
Molecular Wire ◽  
Conjugated Polyelectrolytes ◽  
Chain Reaction ◽  
Amplification Strategy ◽  
Cascade Amplification

A novel cascade amplification strategy that combines the molecular wire effects of CPEs with the signal amplification capability of the HCR has been developed for sensitive DNA detection.

Ribonuclease H Enzyme Activity Detection Based on Hybridization Chain Reaction Amplification and Graphene Oxide Nanosheets Fluorescence Quenching

2020 ◽  
Vol 20 (3) ◽  
pp. 1409-1416
Author(s):  
Cun Liu ◽  
Ying Tang ◽  
Yao Chen ◽  
Jing Deng ◽  
Wen Li ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Fluorescence Quenching ◽  
Rnase H ◽  
Ribonuclease H ◽  
Hybridization Chain Reaction ◽  
Activity Detection ◽  
Chain Reaction ◽  
Replication Process ◽  
Hiv Virus ◽  
Aids Drugs

Ribonuclease H (RNase H) plays key roles in HIV virus replication process and can be used as an important target for anti-AIDS drugs screening. Therefore, we constructed a fluorescent nanosensor based on signal amplification of hybridization chain reaction (HCR) and fluorescence quenching of graphene oxide (GO) nanosheets. The nanosensor provided highly sensitive and selective platform for RNase H activity detection. This method also exhibited a good linear relationship in the range of 0.001–5 U/mL, and the detection limit was 0.0007 U/mL. Our results suggested that the developed system is a promising platform for monitoring the RNase H activity, showing great potential in the biomedical studies and drugs screening.

Sensitive detection of intracellular RNA of human telomerase by using graphene oxide as a carrier to deliver the assembly element of hybridization chain reaction

The Analyst ◽  
2016 ◽  
Vol 141 (9) ◽  
pp. 2727-2732 ◽  
Author(s):  
Zhilu Shi ◽  
Xiafei Zhang ◽  
Rui Cheng ◽  
Baoxin Li ◽  
Yan Jin
Keyword(s):  
Graphene Oxide ◽  
Sensitive Detection ◽  
Hybridization Chain Reaction ◽  
Chain Reaction ◽  
Human Telomerase ◽  
Amplification Strategy

An enzyme-free amplification strategy has been developed for sensitively and specifically detecting intracellular RNA of telomeraseviaGO-aided hybridization chain reaction (HCR).

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