Sandwich-type electrochemical immunoassay based on Co3O4@MnO2-thionine and pseudo-ELISA method toward sensitive detection of alpha fetoprotein

2018 ◽  
Vol 106 ◽  
pp. 179-185 ◽  
Author(s):  
Yaoguang Wang ◽  
Guanhui Zhao ◽  
Huan Wang ◽  
Wei Cao ◽  
Bin Du ◽  
...  
Keyword(s):  
Alpha Fetoprotein ◽  
Sensitive Detection ◽  
Electrochemical Immunoassay ◽  
Elisa Method ◽  
Sandwich Type

scholarly journals A Novel Sandwich-type Electrochemical Aptasensor Based on GR-3D Au and Aptamer-AuNPs-HRP for Sensitive Detection of Oxytetracycline

2017 ◽  
Vol 27 ◽  
pp. 151-152 ◽  
Author(s):  
Su Liu ◽  
Yu Wang ◽  
Wei Xu ◽  
Xueqi Leng ◽  
Hongzhi Wang ◽  
...  
Keyword(s):  
Sensitive Detection ◽  
Electrochemical Aptasensor ◽  
Sandwich Type

Heterogeneous enzyme immunoassay of alpha-fetoprotein in maternal serum by flow-injection amperometric detection of 4-aminophenol

1990 ◽  
Vol 36 (11) ◽  
pp. 1941-1944 ◽  
Author(s):  
Y Xu ◽  
B Halsall ◽  
W R Heineman
Keyword(s):  
Enzyme Immunoassay ◽  
Flow Injection ◽  
Enzymatic Reaction ◽  
Amperometric Detection ◽  
Maternal Serum ◽  
Alpha Fetoprotein ◽  
Serum Samples ◽  
Low Concentrations ◽  
Sandwich Type ◽  
Commercial Kit

Abstract A sandwich-type heterogeneous enzyme immunoassay with flow-injection analysis for alpha-fetoprotein (AFP) in human serum has been developed. 4-Aminophenol, the product of enzymatic reaction, is detected amperometrically. The interassay CV for this electrochemical enzyme immunoassay was less than 8.2%, with a minimum detection limit for AFP of 0.163 micrograms/L. The calibration curve had a linear range of 0.316-100 micrograms/L. Studies with 48 human maternal serum samples, comparing results by this method with those by a commercial kit, showed a good correlation (r = 0.961). This procedure provides an alternative method for determining low concentrations of AFP in human maternal serum.

Electrochemical immunoassay based on polythionine as the signal source for the sensitive detection of carcinoma embryonic antigen

2015 ◽  
Vol 7 (24) ◽  
pp. 10339-10344 ◽  
Author(s):  
Min Chen ◽  
Chengfei Zhao ◽  
Qingshan Xu ◽  
Biqiong Nie ◽  
Liufang Xu ◽  
...  
Keyword(s):  
Human Serum ◽  
Sensitive Detection ◽  
Signal Source ◽  
Electrochemical Immunoassay ◽  
Embryonic Antigen

In this work, an effective immunosensor based on polythionine (PTh) as the signal source was fabricated for the facile and sensitive detection of carcinoma embryonic antigen (CEA) in human serum.

Enzyme-catalyzed silver deposition on irregular-shaped gold nanoparticles for electrochemical immunoassay of alpha-fetoprotein

2012 ◽  
Vol 755 ◽  
pp. 62-68 ◽  
Author(s):  
Wenqiang Lai ◽  
Dianping Tang ◽  
Xiaohua Que ◽  
Junyang Zhuang ◽  
Libing Fu ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Alpha Fetoprotein ◽  
Electrochemical Immunoassay ◽  
Silver Deposition
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