Electrochemiluminescent resonance energy transfer of polymer dots for aptasensing

2018 ◽  
Vol 100 ◽  
pp. 28-34 ◽  
Author(s):  
Feng Sun ◽  
Ziyu Wang ◽  
Yaqiang Feng ◽  
Yixiang Cheng ◽  
Huangxian Ju ◽  
...  
Keyword(s):  
Energy Transfer ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Polymer Dots

scholarly journals Dual resonance energy transfer in triple-component polymer dots to enhance electrochemiluminescence for highly sensitive bioanalysis

2019 ◽  
Vol 10 (28) ◽  
pp. 6815-6820 ◽  
Author(s):  
Ningning Wang ◽  
Ziyu Wang ◽  
Lizhen Chen ◽  
Weiwei Chen ◽  
Yiwu Quan ◽  
...  
Keyword(s):  
Energy Transfer ◽  
Transfer Process ◽  
Energy Transfer Process ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Highly Sensitive ◽  
Polymer Dots ◽  
Dual Resonance

A dual intramolecular electrochemiluminescence resonance energy transfer process is proposed with triple-component Pdots to enhance ECL emission, which greatly improves the ECL efficiency and can be used for sensitive and specific visual quantification of different targets.

scholarly journals Detection of Thrombin Based on Fluorescence Energy Transfer between Semiconducting Polymer Dots and BHQ-Labelled Aptamers

Sensors ◽  
2018 ◽  
Vol 18 (2) ◽  
pp. 589 ◽  
Author(s):  
Yizhang Liu ◽  
Xuekai Jiang ◽  
Wenfeng Cao ◽  
Junyong Sun ◽  
Feng Gao
Keyword(s):  
Energy Transfer ◽  
Detection Limit ◽  
Human Serum ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Experimental Conditions ◽  
Semiconducting Polymer ◽  
Polymer Dots ◽  
Nanoprecipitation Method ◽  
Semiconducting Polymer Dots

Carboxyl-functionalized semiconducting polymer dots (Pdots) were synthesized as an energy donor by the nanoprecipitation method. A black hole quenching dye (BHQ-labelled thrombin aptamers) was used as the energy acceptor, and fluorescence resonance energy transfer between the aptamers and Pdots was used for fluorescence quenching of the Pdots. The addition of thrombin restored the fluorescence intensity. Under the optimized experimental conditions, the fluorescence of the system was restored to the maximum when the concentration of thrombin reached 130 nM, with a linear range of 0–50 nM (R2 = 0.990) and a detection limit of 0.33 nM. This sensor was less disturbed by impurities, showing good specificity and signal response to thrombin, with good application in actual samples. The detection of human serum showed good linearity in the range of 0–30 nM (R2 = 0.997), with a detection limit of 0.56 nM and a recovery rate of 96.2–104.1%, indicating that this fluorescence sensor can be used for the detection of thrombin content in human serum.

Quantum Dot Bioconjugates as Energy Donors in Fluorescence Resonance Energy Transfer Assays

2003 ◽  
Vol 773 ◽  
Author(s):  
Aaron R. Clapp ◽  
Igor L. Medintz ◽  
J. Matthew Mauro ◽  
Hedi Mattoussi
Keyword(s):  
Energy Transfer ◽  
Quantum Dot ◽  
Organic Dyes ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Genetically Engineered ◽  
Molar Ratio ◽  
Binding Assays ◽  
Specific Sequence ◽  
Donor Acceptor

AbstractLuminescent CdSe-ZnS core-shell quantum dot (QD) bioconjugates were used as energy donors in fluorescent resonance energy transfer (FRET) binding assays. The QDs were coated with saturating amounts of genetically engineered maltose binding protein (MBP) using a noncovalent immobilization process, and Cy3 organic dyes covalently attached at a specific sequence to MBP were used as energy acceptor molecules. Energy transfer efficiency was measured as a function of the MBP-Cy3/QD molar ratio for two different donor fluorescence emissions (different QD core sizes). Apparent donor-acceptor distances were determined from these FRET studies, and the measured distances are consistent with QD-protein conjugate dimensions previously determined from structural studies.

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