An enzyme-free surface plasmon resonance biosensing strategy for detection of DNA and small molecule based on nonlinear hybridization chain reaction

2017 ◽  
Vol 87 ◽  
pp. 345-351 ◽  
Author(s):  
Xiaojuan Ding ◽  
Wei Cheng ◽  
Yujian Li ◽  
Jiangling Wu ◽  
Xinmin Li ◽  
...  
Keyword(s):  
Free Surface ◽  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Small Molecule ◽  
Plasmon Resonance ◽  
Hybridization Chain Reaction ◽  
Chain Reaction

A surface plasmon resonance assay coupled with a hybridization chain reaction for amplified detection of DNA and small molecules

2014 ◽  
Vol 50 (39) ◽  
pp. 5049-5052 ◽  
Author(s):  
Xuemei Li ◽  
Yan Wang ◽  
Linlin Wang ◽  
Qingli Wei
Keyword(s):  
Surface Plasmon Resonance ◽  
Small Molecules ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Biological Samples ◽  
Detection System ◽  
High Sensitivity ◽  
Hybridization Chain Reaction ◽  
Chain Reaction

A surface plasmon resonance (SPR) detection system based on a hybridization chain reaction (HCR) was developed for amplified detection of DNA and small molecules with high sensitivity. This methodology is capable of detecting the target in complicated biological samples and can be further extended to the detection of other proteins or biomarkers.

scholarly journals Biosensing Amplification by Hybridization Chain Reaction on Phase-Sensitive Surface Plasmon Resonance

Biosensors ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 75
Author(s):  
Ching-Hsu Yang ◽  
Tzu-Heng Wu ◽  
Chia-Chen Chang ◽  
Hui-Yun Lo ◽  
Hui-Wen Liu ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Dna Sequences ◽  
Plasmon Resonance ◽  
High Efficiency ◽  
Specific Binding ◽  
Dna Amplification ◽  
Hybridization Chain Reaction ◽  
Chain Reaction ◽  
Phase Sensitive

Surface Plasmon Resonance (SPR) is widely used in biological and chemical sensing with fascinating properties. However, the application of SPR to detect trace targets is hampered by non-specific binding and poor signal. A variety of approaches for amplification have been explored to overcome this deficiency including DNA aptamers as versatile target detection tools. Hybridization chain reaction (HCR) is a high-efficiency enzyme-free DNA amplification method operated at room temperature, in which two stable species of DNA hairpins coexist in solution until the introduction of the initiator strand triggers a cascade of hybridization events. At an optimal salt condition, as the concentrations of H1 and H2 increased, the HCR signals were enhanced, leading to signal amplification reaching up to 6.5-fold of the detection measure at 30 min. This feature enables DNA to act as an amplifying transducer for biosensing applications to provide an enzyme-free alternative that can easily detect complex DNA sequences. Improvement of more diverse recognition events can be achieved by integrating HCR with a phase-sensitive SPR (pSPR)-tested aptamer stimulus. This work seeks to establish pSPR aptamer system for highly informative sensing by means of an amplification HCR. Thus, combining pSPR and HCR technologies provide an expandable platform for sensitive biosensing.

Surface plasmon resonance detection of small molecule using split aptamer fragments

2011 ◽  
Vol 156 (2) ◽  
pp. 893-898 ◽  
Author(s):  
Qing Wang ◽  
Jiahao Huang ◽  
Xiaohai Yang ◽  
Kemin Wang ◽  
Leiliang He ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Small Molecule ◽  
Plasmon Resonance ◽  
Resonance Detection

scholarly journals Small molecule microarray screening methodology based on surface plasmon resonance imaging

2019 ◽  
Vol 12 (8) ◽  
pp. 2111-2117 ◽  
Author(s):  
Vikramjeet Singh ◽  
Kuldeep Singh ◽  
Amita Nand ◽  
Huanqin Dai ◽  
Jianguo Wang ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Small Molecule ◽  
Plasmon Resonance ◽  
Surface Plasmon Resonance Imaging ◽  
Resonance Imaging ◽  
Microarray Screening

scholarly journals A surface plasmon resonance-based assay for small molecule inhibitors of human cyclophilin A

2005 ◽  
Vol 345 (2) ◽  
pp. 214-226 ◽  
Author(s):  
Martin A. Wear ◽  
Alan Patterson ◽  
Kirk Malone ◽  
Colin Dunsmore ◽  
Nicholas J. Turner ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Small Molecule ◽  
Plasmon Resonance ◽  
Small Molecule Inhibitors ◽  
Cyclophilin A

scholarly journals Multifunctional Biosensor Based on Localized Surface Plasmon Resonance for Monitoring Small Molecule–Protein Interaction

ACS Nano ◽  
2014 ◽  
Vol 8 (8) ◽  
pp. 7958-7967 ◽  
Author(s):  
Joana Rafaela Lara Guerreiro ◽  
Maj Frederiksen ◽  
Vladimir E. Bochenkov ◽  
Victor De Freitas ◽  
Maria Goreti Ferreira Sales ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Small Molecule ◽  
Protein Interaction ◽  
Plasmon Resonance ◽  
Localized Surface Plasmon Resonance ◽  
Localized Surface Plasmon
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