A superstructure-based electrochemical assay for signal-amplified detection of DNA methyltransferase activity

2016 ◽  
Vol 86 ◽  
pp. 927-932 ◽  
Author(s):  
Hui Zhang ◽  
Yin Yang ◽  
Huilei Dong ◽  
Chenxin Cai
Keyword(s):  
Dna Methyltransferase ◽  
Methyltransferase Activity ◽  
Electrochemical Assay

scholarly journals Reduced Genomic Cytosine Methylation and Defective Cellular Differentiation in Embryonic Stem Cells Lacking CpG Binding Protein

2005 ◽  
Vol 25 (12) ◽  
pp. 4881-4891 ◽  
Author(s):  
Diana L. Carlone ◽  
Jeong-Heon Lee ◽  
Suzanne R. L. Young ◽  
Erika Dobrota ◽  
Jill Sergesketter Butler ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Dna Methyltransferase ◽  
Cellular Differentiation ◽  
Cytosine Methylation ◽  
Epigenetic Modification ◽  
Binding Protein ◽  
Embryonic Stem ◽  
Growth Media ◽  
Methyltransferase Activity

ABSTRACT Cytosine methylation at CpG dinucleotides is a critical epigenetic modification of mammalian genomes. CpG binding protein (CGBP) exhibits a unique DNA-binding specificity for unmethylated CpG motifs and is essential for early murine development. Embryonic stem cell lines deficient for CGBP were generated to further examine CGBP function. CGBP − / − cells are viable but show an increased rate of apoptosis and are unable to achieve in vitro differentiation following removal of leukemia inhibitory factor from the growth media. Instead, CGBP − / − embryonic stem cells remain undifferentiated as revealed by persistent expression of the pluripotent markers Oct4 and alkaline phosphatase. CGBP − / − cells exhibit a 60 to 80% decrease in global cytosine methylation, including hypo-methylation of repetitive elements, single-copy genes, and imprinted genes. Total DNA methyltransferase activity is reduced by 30 to 60% in CGBP − / − cells, and expression of the maintenance DNA methyltransferase 1 protein is similarly reduced. However, de novo DNA methyltransferase activity is normal. Nearly all aspects of the pleiotropic CGBP − / − phenotype are rescued by introduction of a CGBP expression vector. Hence, CGBP is essential for normal epigenetic modification of the genome by cytosine methylation and for cellular differentiation, consistent with the requirement for CGBP during early mammalian development.

scholarly journals DNA methyltransferase activity in the early stages of a sea urchin embryo Evidence of differential control

FEBS Letters ◽  
1995 ◽  
Vol 361 (1) ◽  
pp. 115-117 ◽  
Author(s):  
Luisa Tosi ◽  
Francesco Aniello ◽  
Giuseppe Geraci ◽  
Margherita Branno
Keyword(s):  
Sea Urchin ◽  
Dna Methyltransferase ◽  
Methyltransferase Activity ◽  
Early Stages ◽  
Sea Urchin Embryo ◽  
Differential Control

scholarly journals Evolutionary analysis indicates that DNA alkylation damage is a byproduct of cytosine DNA methyltransferase activity

2018 ◽  
Vol 50 (3) ◽  
pp. 452-459 ◽  
Author(s):  
Silvana Rošić ◽  
Rachel Amouroux ◽  
Cristina E. Requena ◽  
Ana Gomes ◽  
Max Emperle ◽  
...  
Keyword(s):  
Dna Methyltransferase ◽  
Dna Alkylation ◽  
Evolutionary Analysis ◽  
Methyltransferase Activity ◽  
Alkylation Damage

Sensitive detection of DNA methyltransferase activity by transcription-mediated duplex-specific nuclease-assisted cyclic signal amplification

2015 ◽  
Vol 51 (73) ◽  
pp. 13968-13971 ◽  
Author(s):  
Yan Zhang ◽  
Wen-jing Xu ◽  
Ya-ping Zeng ◽  
Chun-yang Zhang
Keyword(s):  
Signal Amplification ◽  
Dna Methyltransferase ◽  
Sensitive Detection ◽  
Methyltransferase Activity

We develop transcription-mediated duplex-specific nuclease-assisted cyclic signal amplification for sensitive detection of DNA methyltransferase activity.

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