Fluorescent molecularly imprinted polymers as plastic antibodies for selective labeling and imaging of hyaluronan and sialic acid on fixed and living cells

2017 ◽  
Vol 88 ◽  
pp. 85-93 ◽  
Author(s):  
Maria Panagiotopoulou ◽  
Stephanie Kunath ◽  
Paulina Ximena Medina-Rangel ◽  
Karsten Haupt ◽  
Bernadette Tse Sum Bui
Keyword(s):  
Sialic Acid ◽  
Molecularly Imprinted Polymers ◽  
Living Cells ◽  
Selective Labeling ◽  
Molecularly Imprinted ◽  
Imprinted Polymers

scholarly journals Evaluation of the Impact of Imprinted Polymer Particles on Morphology and Motility of Breast Cancer Cells by Using Digital Holographic Cytometry

2020 ◽  
Vol 10 (3) ◽  
pp. 750 ◽  
Author(s):  
Megha Patel ◽  
Marek Feith ◽  
Birgit Janicke ◽  
Kersti Alm ◽  
Zahra El-Schich
Keyword(s):  
Breast Cancer ◽  
Sialic Acid ◽  
Cancer Cells ◽  
Cell Lines ◽  
Cell Morphology ◽  
Molecularly Imprinted Polymers ◽  
Breast Cancer Cells ◽  
Molecularly Imprinted ◽  
Imprinted Polymers ◽  
Mcf 7

Breast cancer is the second most common cancer type worldwide and breast cancer metastasis accounts for the majority of breast cancer-related deaths. Tumour cells produce increased levels of sialic acid (SA) that terminates the monosaccharide on glycan chains of the glycosylated proteins. SA can contribute to cellular recognition, cancer invasiveness and increase the metastatic potential of cancer cells. SA-templated molecularly imprinted polymers (MIPs) have been proposed as promising reporters for specific targeting of cancer cells when deployed in nanoparticle format. The sialic acid-molecularly imprinted polymers (SA-MIPs), which use SA for the generation of binding sites through which the nanoparticles can target and stain breast cancer cells, opens new strategies for efficient diagnostic tools. This study aims at monitoring the effects of SA-MIPs on morphology and motility of the epithelial type MCF-7 and the highly metastatic MDAMB231 breast cancer cell lines, using digital holographic cytometry (DHC). DHC is a label-free technique that is used in cell morphology studies of e.g., cell volume, area and thickness as well as in motility studies. Here, we show that MCF-7 cells move slower than MDAMB231 cells. We also show that SA-MIPs have an effect on cell morphology, motility and viability of both cell lines. In conclusion, by using DH microscopy, we could detect SA-MIPs impact on different breast cancer cells regarding morphology and motility.

Potentiometric analysis of sialic acid with a flexible carbon cloth based on boronate affinity and molecularly imprinted polymers

The Analyst ◽  
2019 ◽  
Vol 144 (21) ◽  
pp. 6432-6437 ◽  
Author(s):  
Yanli Zhou ◽  
Huijie Huangfu ◽  
Jie Yang ◽  
Hui Dong ◽  
Lantao liu ◽  
...  
Keyword(s):  
Sialic Acid ◽  
Molecularly Imprinted Polymers ◽  
Boronic Acid ◽  
Potentiometric Sensor ◽  
Carbon Cloth ◽  
Molecularly Imprinted ◽  
Imprinted Polymers ◽  
Potentiometric Analysis

A potentiometric sensor for sialic acid detection was designed based on a boronic acid-containing MIP modified carbon cloth electrode.

scholarly journals Different expression levels of glycans on leukemic cells—a novel screening method with molecularly imprinted polymers (MIP) targeting sialic acid

Tumor Biology ◽  
2016 ◽  
Vol 37 (10) ◽  
pp. 13763-13768 ◽  
Author(s):  
Zahra El-Schich ◽  
Mohammad Abdullah ◽  
Sudhirkumar Shinde ◽  
Nishtman Dizeyi ◽  
Anders Rosén ◽  
...  
Keyword(s):  
Sialic Acid ◽  
Molecularly Imprinted Polymers ◽  
Screening Method ◽  
Leukemic Cells ◽  
Molecularly Imprinted ◽  
Imprinted Polymers ◽  
Expression Levels

scholarly journals Determination of sialic acid in serum samples by dispersive solid-phase extraction based on boronate-affinity magnetic hollow molecularly imprinted polymer sorbent

RSC Advances ◽  
2019 ◽  
Vol 9 (10) ◽  
pp. 5394-5401 ◽  
Author(s):  
Wei Huang ◽  
Xingyu Hou ◽  
Yukui Tong ◽  
Miaomiao Tian
Keyword(s):  
Sialic Acid ◽  
Molecularly Imprinted Polymers ◽  
Solid Phase ◽  
Phase Extraction ◽  
Dispersive Solid Phase Extraction ◽  
Imprinted Polymer ◽  
Serum Samples ◽  
Molecularly Imprinted ◽  
Imprinted Polymers

Boronate-affinity magnetic hollow molecularly imprinted polymers were prepared with sialic acid (SA) as a template to selectively extract SA from serum samples coupled with HPLC-UV.

Regioselective Ester Hydrolysis of Sialic Acid Derivatives Catalyzed by Molecularly Imprinted Polymers

Heterocycles ◽  
2009 ◽  
Vol 78 (12) ◽  
pp. 2943
Author(s):  
Kiyoshi Ikeda ◽  
Takashi Katsumata ◽  
Yuji Sugiyama ◽  
Keisuke Miyamoto ◽  
Kiyoshi Tanaka ◽  
...  
Keyword(s):  
Sialic Acid ◽  
Molecularly Imprinted Polymers ◽  
Ester Hydrolysis ◽  
Molecularly Imprinted ◽  
Imprinted Polymers ◽  
Hydrolysis Of

scholarly journals Sialic acid as a biomarker studied in breast cancer cells using fluorescent molecularly imprinted polymers, MIPs

2020 ◽  
Author(s):  
Zahra El-Schich ◽  
Yuecheng Zhang ◽  
Tommy Göransson ◽  
Nishtman Dizeyi ◽  
Jenny Persson ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Sialic Acid ◽  
Confocal Microscopy ◽  
Cancer Cells ◽  
Tumor Cells ◽  
Molecularly Imprinted Polymers ◽  
Breast Cancer Cells ◽  
Primary Tumors ◽  
Molecularly Imprinted ◽  
Imprinted Polymers

Abstract Sialylations are post-translational modifications of proteins and lipids that play important roles in many cellular events, including cell-cell interactions, proliferation and migration. Tumor cells express high levels of sialic acid (SA), which are often associated with the increased invasive potential in clinical tumors correlating with poor prognosis. To overcome the lack of natural SA-receptors, such as antibodies and lectins with high enough specificity and sensitivity, we have used molecularly imprinted polymers (MIPs), or “plastic antibodies”, as nanoprobes. Since high expression of EpCAM in primary tumors often is associated with proliferation and a more aggressive phenotype, the expression and of epithelial cell adhesion molecule (EpCAM) CD44 was initially analyzed. The SA-MIPs were used here for the detection of SA on the cell surface of breast cancer cells. Lectins that specifically bind to the α-2,3 SA and α-2,6 SA variants, respectively, were used for analysis of SA expression with both flow cytometry and confocal microscopy. Here we show a correlation of EpCAM and SA expression when using the SA-MIPs for detection of SA. We also demonstrate the binding pattern of the SA-MIPs on the breast cancer cell lines using confocal microscopy. Pre-incubation of the SA-MIPs with SA-derivatives as inhibitors could reduce the binding of the SA-MIPs to the tumor cells, indicating the specificity of the SA-MIPs. In conclusion, the SA-MIPs may be a new powerful tool in the SA-analysis of breast cancer cells.

scholarly journals Determination of cytokine regulated glycan expression by using molecularly imprinted polymers targeting sialic acid

2019 ◽  
Vol 2019 ◽  
Author(s):  
Yuecheng Zhang ◽  
Kushtrim Llapashtica ◽  
Sudhirkumar Shinde ◽  
Borje Sellergren ◽  
Zahra El-Schich ◽  
...  
Keyword(s):  
Sialic Acid ◽  
Molecularly Imprinted Polymers ◽  
Molecularly Imprinted ◽  
Imprinted Polymers

scholarly journals Sialic Acid as a Biomarker Studied in Breast Cancer Cell Lines In Vitro Using Fluorescent Molecularly Imprinted Polymers

2021 ◽  
Vol 11 (7) ◽  
pp. 3256
Author(s):  
Zahra El-Schich ◽  
Yuecheng Zhang ◽  
Tommy Göransson ◽  
Nishtman Dizeyi ◽  
Jenny L. Persson ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Sialic Acid ◽  
Confocal Microscopy ◽  
Cancer Cells ◽  
Tumor Cells ◽  
Molecularly Imprinted Polymers ◽  
Breast Cancer Cells ◽  
Breast Cancer Cell Lines ◽  
Molecularly Imprinted ◽  
Imprinted Polymers

Sialylations are post-translational modifications of proteins and lipids that play important roles in many cellular events, including cell-cell interactions, proliferation, and migration. Tumor cells express high levels of sialic acid (SA), which are often associated with the increased invasive potential in clinical tumors, correlating with poor prognosis. To overcome the lack of natural SA-receptors, such as antibodies and lectins with high enough specificity and sensitivity, we have used molecularly imprinted polymers (MIPs), or “plastic antibodies”, as nanoprobes. Because high expression of epithelial cell adhesion molecule (EpCAM) in primary tumors is often associated with proliferation and a more aggressive phenotype, the expression of EpCAM and CD44 was initially analyzed. The SA-MIPs were used for the detection of SA on the cell surface of breast cancer cells. Lectins that specifically bind to the a-2,3 SA and a-2,6 SA variants were used for analysis of SA expression, with both flow cytometry and confocal microscopy. Here we show a correlation of EpCAM and SA expression when using the SA-MIPs for detection of SA. We also demonstrate the binding pattern of the SA-MIPs on the breast cancer cell lines using confocal microscopy. Pre-incubation of the SA-MIPs with SA-derivatives as inhibitors could reduce the binding of the SA-MIPs to the tumor cells, indicating the specificity of the SA-MIPs. In conclusion, the SA-MIPs may be a new powerful tool in the diagnostic analysis of breast cancer cells.

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