Ultrasensitive electrochemical detection of protein tyrosine kinase-7 by gold nanoparticles and methylene blue assisted signal amplification

2016 ◽  
Vol 83 ◽  
pp. 39-44 ◽  
Author(s):  
Xiangmin Miao ◽  
Zongbing Li ◽  
Aihua Zhu ◽  
Zhaozhong Feng ◽  
Jun Tian ◽  
...  
Keyword(s):  
Methylene Blue ◽  
Gold Nanoparticles ◽  
Tyrosine Kinase ◽  
Electrochemical Detection ◽  
Signal Amplification ◽  
Protein Tyrosine Kinase ◽  
Protein Tyrosine ◽  
Protein Tyrosine Kinase 7

scholarly journals Multi-carbon dots and aptamer based signal amplification ratiometric fluorescence probe for protein tyrosine kinase 7 detection

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Yunsu Ma ◽  
Yuan Wang ◽  
Yongjie Liu ◽  
Lujia Shi ◽  
Dongzhi Yang
Keyword(s):  
Tyrosine Kinase ◽  
Carbon Dots ◽  
Signal Amplification ◽  
Protein Tyrosine Kinase ◽  
Fluorescence Probe ◽  
Ratiometric Fluorescence ◽  
Détection Signal ◽  
Protein Tyrosine ◽  
Protein Tyrosine Kinase 7 ◽  
Detection Signal

Abstract Background Protein tyrosine kinase 7 (PTK 7) is a membrane receptor, which can be found in various kinds of cancers. In view of this, detection of PTK 7 in the peripheral circulation would be an effective way for the early diagnosis of cancer. Results In this work, a multi-carbon dots and aptamer-based signal amplification ratiometric fluorescence probe was developed. The fluorescence of the aptamer-modified y-CDs and b-CDs were respectively chosen as the detection signal and interior label. The fluorescence of y-CDs was quenched by Fe3O4 and cDNA (complement to aptamer) compound without PTK 7, but recovered by the addition of PTK 7. Then, the free aptamer was cut by DNase I, which amplified the detection signal. The ratiometric fluorescence sensor for PTK 7 was established with the LOD of 0.016 ng mL−1. Conclusions Summary, a multi-carbon dots and aptamer-based signal amplification ratiometric fluorescence probe was developed for the detection of protein tyrosine kinase 7. The developed probe was applied to PTK 7 detection in MCF-7 cells and human serum with satisfying results, thus indicating that this probe has huge potential in clinical practice.

scholarly journals Luminescence switch-on detection of protein tyrosine kinase-7 using a G-quadruplex-selective probe

2015 ◽  
Vol 6 (7) ◽  
pp. 4284-4290 ◽  
Author(s):  
Sheng Lin ◽  
Wei Gao ◽  
Zeru Tian ◽  
Chao Yang ◽  
Lihua Lu ◽  
...  
Keyword(s):  
Tyrosine Kinase ◽  
Protein Tyrosine Kinase ◽  
Protein Tyrosine ◽  
Detection Assay ◽  
G Quadruplex ◽  
Protein Tyrosine Kinase 7

A novel luminescent G-quadruplex-selective iridium(iii) complex was employed in a G-quadruplex-based detection assay for PTK7.

scholarly journals The Wnt/Planar Cell Polarity Protein-tyrosine Kinase-7 (PTK7) Is a Highly Efficient Proteolytic Target of Membrane Type-1 Matrix Metalloproteinase

2010 ◽  
Vol 285 (46) ◽  
pp. 35740-35749 ◽  
Author(s):  
Vladislav S. Golubkov ◽  
Alexei V. Chekanov ◽  
Piotr Cieplak ◽  
Alexander E. Aleshin ◽  
Andrei V. Chernov ◽  
...  
Keyword(s):  
Matrix Metalloproteinase ◽  
Tyrosine Kinase ◽  
Cell Polarity ◽  
Protein Tyrosine Kinase ◽  
Planar Cell Polarity ◽  
Protein Tyrosine ◽  
Highly Efficient ◽  
Membrane Type ◽  
Protein Tyrosine Kinase 7
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