scholarly journals Real-time monitoring of macromolecular biosensing probe self-assembly and on-chip ELISA using impedimetric microsensors

2016 ◽  
Vol 81 ◽  
pp. 401-407 ◽  
Author(s):  
Faheng Zang ◽  
Konstantinos Gerasopoulos ◽  
Xiao Zhu Fan ◽  
Adam D. Brown ◽  
James N. Culver ◽  
...  
Keyword(s):  
Real Time ◽  
Self Assembly ◽  
Real Time Monitoring ◽  
On Chip

Real time monitoring of superparamagnetic nanoparticle self-assembly on surfaces of magnetic recording media

2014 ◽  
Vol 115 (17) ◽  
pp. 17B513 ◽  
Author(s):  
L. Ye ◽  
B. Qi ◽  
T. Pearson ◽  
Y. Cordeau ◽  
O. T. Mefford ◽  
...  
Keyword(s):  
Real Time ◽  
Magnetic Recording ◽  
Self Assembly ◽  
Recording Media ◽  
Magnetic Recording Media ◽  
Real Time Monitoring

scholarly journals On chip real time monitoring of B-cells hybridoma secretion of immunoglobulin

2011 ◽  
Vol 26 (5) ◽  
pp. 2728-2732 ◽  
Author(s):  
Sarah Milgram ◽  
Sandra Cortes ◽  
Marie-Bernadette Villiers ◽  
Patrice Marche ◽  
Arnaud Buhot ◽  
...  
Keyword(s):  
B Cells ◽  
Real Time ◽  
Real Time Monitoring ◽  
On Chip

scholarly journals Regenerable Bead-Based Microfluidic Device with integrated THIN-Film Photodiodes for Real Time Monitoring of DNA Detection

Proceedings ◽  
2018 ◽  
Vol 2 (13) ◽  
pp. 953
Author(s):  
Catarina R. F. Caneira ◽  
Denis R. Santos ◽  
Virginia Chu ◽  
João P. Conde
Keyword(s):  
Thin Film ◽  
Real Time ◽  
Microfluidic Device ◽  
Low Cost ◽  
High Sensitivity ◽  
Significant Loss ◽  
Real Time Monitoring ◽  
Target Dna ◽  
On Chip ◽  
Biosensing System

Nanoporous microbead-based microfluidic systems for biosensing applications allow enhanced sensitivities, while being low cost and amenable for miniaturization. The regeneration of the microfluidic biosensing system results in a further decrease in costs while the integration of on-chip signal transduction enhances portability. Here, we present a regenerable bead-based microfluidic device, with integrated thin-film photodiodes, for real-time monitoring of molecular recognition between a target DNA and complementary DNA (cDNA). High-sensitivity assay cycles could be performed without significant loss of probe DNA density and activity, demonstrating the potential for reusability, portability and reproducibility of the system.

scholarly journals Multi-Organs-on-Chips: Towards Long-Term Biomedical Investigations

Molecules ◽  
2019 ◽  
Vol 24 (4) ◽  
pp. 675 ◽  
Author(s):  
Yi Zhao ◽  
Ranjith Kankala ◽  
Shi-Bin Wang ◽  
Ai-Zheng Chen
Keyword(s):  
Real Time ◽  
Three Dimensional ◽  
Drug Efficacy ◽  
Dynamic Responses ◽  
Physical Parameters ◽  
Real Time Monitoring ◽  
Multiple Organs ◽  
On Chip

With advantageous features such as minimizing the cost, time, and sample size requirements, organ-on-a-chip (OOC) systems have garnered enormous interest from researchers for their ability for real-time monitoring of physical parameters by mimicking the in vivo microenvironment and the precise responses of xenobiotics, i.e., drug efficacy and toxicity over conventional two-dimensional (2D) and three-dimensional (3D) cell cultures, as well as animal models. Recent advancements of OOC systems have evidenced the fabrication of ‘multi-organ-on-chip’ (MOC) models, which connect separated organ chambers together to resemble an ideal pharmacokinetic and pharmacodynamic (PK-PD) model for monitoring the complex interactions between multiple organs and the resultant dynamic responses of multiple organs to pharmaceutical compounds. Numerous varieties of MOC systems have been proposed, mainly focusing on the construction of these multi-organ models, while there are only few studies on how to realize continual, automated, and stable testing, which still remains a significant challenge in the development process of MOCs. Herein, this review emphasizes the recent advancements in realizing long-term testing of MOCs to promote their capability for real-time monitoring of multi-organ interactions and chronic cellular reactions more accurately and steadily over the available chip models. Efforts in this field are still ongoing for better performance in the assessment of preclinical attributes for a new chemical entity. Further, we give a brief overview on the various biomedical applications of long-term testing in MOCs, including several proposed applications and their potential utilization in the future. Finally, we summarize with perspectives.

Multiwavelength Photosensor for On-Chip Real-Time Monitoring of Fluorescence and Turbidity

2009 ◽  
Vol 48 (6) ◽  
pp. 067003 ◽  
Author(s):  
Yuki Maruyama ◽  
Makoto Ishida ◽  
Kazuaki Sawada
Keyword(s):  
Real Time ◽  
Real Time Monitoring ◽  
On Chip

High-Throughput, Real-Time Monitoring of the Self-Assembly of DNA Nanostructures by FRET Spectroscopy

2008 ◽  
Vol 47 (11) ◽  
pp. 2135-2137 ◽  
Author(s):  
Barbara Saccà ◽  
Rebecca Meyer ◽  
Udo Feldkamp ◽  
Hendrik Schroeder ◽  
Christof M. Niemeyer
Keyword(s):  
Real Time ◽  
High Throughput ◽  
Self Assembly ◽  
The Self ◽  
Dna Nanostructures ◽  
Real Time Monitoring

scholarly journals Barrier-on-a-Chip with a Modular Architecture and Integrated Sensors for Real-Time Measurement of Biological Barrier Function

Micromachines ◽  
2021 ◽  
Vol 12 (7) ◽  
pp. 816
Author(s):  
Patrícia Zoio ◽  
Sara Lopes-Ventura ◽  
Abel Oliva
Keyword(s):  
Real Time ◽  
Barrier Function ◽  
Imaging Techniques ◽  
Real Time Monitoring ◽  
Modular Architecture ◽  
Element Analysis ◽  
Cell Culture Techniques ◽  
Biological Barriers ◽  
On Chip

Biological barriers are essential for the maintenance of organ homeostasis and their dysfunction is responsible for many prevalent diseases. Advanced in vitro models of biological barriers have been developed through the combination of 3D cell culture techniques and organ-on-chip (OoC) technology. However, real-time monitoring of tissue function inside the OoC devices has been challenging, with most approaches relying on off-chip analysis and imaging techniques. In this study, we designed and fabricated a low-cost barrier-on-chip (BoC) device with integrated electrodes for the development and real-time monitoring of biological barriers. The integrated electrodes were used to measure transepithelial electrical resistance (TEER) during tissue culture, thereby quantitatively evaluating tissue barrier function. A finite element analysis was performed to study the sensitivity of the integrated electrodes and to compare them with conventional systems. As proof-of-concept, a full-thickness human skin model (FTSm) was grown on the developed BoC, and TEER was measured on-chip during the culture. After 14 days of culture, the barrier tissue was challenged with a benchmark irritant and its impact was evaluated on-chip through TEER measurements. The developed BoC with an integrated sensing capability represents a promising tool for real-time assessment of barrier function in the context of drug testing and disease modelling.

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