Hetero-enzyme-based two-round signal amplification strategy for trace detection of aflatoxin B1 using an electrochemical aptasensor

2016 ◽  
Vol 80 ◽  
pp. 574-581 ◽  
Author(s):  
Wanli Zheng ◽  
Jun Teng ◽  
Lin Cheng ◽  
Yingwang Ye ◽  
Daodong Pan ◽  
...  
Keyword(s):  
Aflatoxin B1 ◽  
Signal Amplification ◽  
Trace Detection ◽  
Electrochemical Aptasensor ◽  
Amplification Strategy

scholarly journals A novel electrochemical aptasensor for fumonisin B1 determination using DNA and exonuclease-I as signal amplification strategy

BMC Chemistry ◽  
2019 ◽  
Vol 13 (1) ◽  
Author(s):  
Min Wei ◽  
Fei Zhao ◽  
Shuo Feng ◽  
Huali Jin
Keyword(s):  
Incubation Time ◽  
Electrode Surface ◽  
Signal Amplification ◽  
Limit Of Detection ◽  
Fumonisin B1 ◽  
Electrochemical Aptasensor ◽  
Experimental Conditions ◽  
Exonuclease I ◽  
Double Stranded Dna ◽  
Amplification Strategy

Abstract In this work, using DNA and exonuclease-I (Exo-I) as signal amplification strategy, a novel and facile electrochemical aptasensor was constructed for fumonisin B1 (FB1) detection. The G-rich complementary DNA (cDNA) was immobilized onto the electrode surface. Then, aptamer of FB1 was hybridized with cDNA to form double-stranded DNA. In the absence of FB1, double-stranded DNA and G-rich cDNA on the electrode surface promoted effectively methylene blue (MB) enrichment and amplified the initial electrochemical response. In the presence of FB1, the combination of aptamer and FB1 led to the release of aptamer from the electrode surface and the expose of 3′ end of single-stranded cDNA. When Exo-I was added onto the electrode surface, the single-stranded cDNA was degraded in the 3′–5′ direction. The decrease of double-stranded DNA and G-rich cDNA resulted in the less access of MB to the electrode surface, which decreased the electrochemical signal. The experimental conditions including incubation time of FB1, the amount of Exo-I and incubation time of Exo-I were optimized. Under the optimal conditions, the linear relationship between the change of peak current and the logarithmic concentration of FB1 was observed in the range of 1.0 × 10−3–1000 ng mL−1 with a low limit of detection of 0.15 pg mL−1. The experimental results showed that the prepared aptasensor had acceptable specificity, reproducibility, repeatability and stability. Therefore, this proposed aptasensor has a potential application in the food safety detection.

Homogeneous electrochemical aptasensor based on a dual amplification strategy for sensitive detection of profenofos residues

2018 ◽  
Vol 42 (17) ◽  
pp. 14642-14647 ◽  
Author(s):  
Yancui Jiao ◽  
Jiayun Fu ◽  
Wenjie Hou ◽  
Zhaoqiang Shi ◽  
Yemin Guo ◽  
...  
Keyword(s):  
Signal Amplification ◽  
Sensitive Detection ◽  
Homogeneous Type ◽  
Electrochemical Aptasensor ◽  
Dual Amplification ◽  
Amplification Strategy

A homogeneous type of electrochemical aptasensor was designed based upon the principle of target-induced and tool enzyme-assisted signal amplification, which was employed for the detection of profenofos residues.

Ultrasensitive electrochemical aptasensor for ochratoxin A based on two-level cascaded signal amplification strategy

2014 ◽  
Vol 96 ◽  
pp. 7-13 ◽  
Author(s):  
Xingwang Yang ◽  
Jing Qian ◽  
Ling Jiang ◽  
Yuting Yan ◽  
Kan Wang ◽  
...  
Keyword(s):  
Ochratoxin A ◽  
Signal Amplification ◽  
Electrochemical Aptasensor ◽  
Amplification Strategy

scholarly journals A novel electrochemical aptasensor for fumonisin B1 determination using DNA and exonuclease-I as signal amplification strategy

2019 ◽  
Author(s):  
Min Wei ◽  
Fei Zhao ◽  
Shuo Feng ◽  
Huali Jin
Keyword(s):  
Incubation Time ◽  
Electrode Surface ◽  
Signal Amplification ◽  
Limit Of Detection ◽  
Fumonisin B1 ◽  
Electrochemical Aptasensor ◽  
Experimental Conditions ◽  
Exonuclease I ◽  
Double Stranded Dna ◽  
Amplification Strategy

Abstract In this work, using DNA and exonuclease-I (Exo-I) as signal amplification strategy, a novel and facile electrochemical aptasensor was constructed for fumonisin B1 (FB1) detection. The G-rich complementary DNA (cDNA) was immobilized onto the electrode surface. Then, aptamer of FB1 was hybridized with cDNA to form double-stranded DNA. In the absence of FB1, double-stranded DNA and G-rich cDNA on the electrode surface promoted effectively methylene blue (MB) enrichment and amplified the initial electrochemical response. In the presence of FB1, the combination of aptamer and FB1 led to the release of aptamer from the electrode surface and the expose of 3' end of single-stranded cDNA. When Exo-I was added onto the electrode surface, the single-stranded cDNA was degraded in the 3’-5’ direction. The decrease of double-stranded DNA and G-rich cDNA resulted in the less access of MB to the electrode surface, which decreased the electrochemical signal. The experimental conditions including incubation time of FB1, the amount of Exo-I and incubation time of Exo-I were optimized. Under the optimal conditions, the linear relationship between the change of peak current and the logarithmic concentration of FB1 was observed in the range of 1.0×10-3-1000ng·mL−1 with a low limit of detection of 0.15 pg·mL−1. The experimental results showed that the prepared aptasensor had acceptable specificity, reproducibility, repeatability and stability. Therefore, this proposed aptasensor has a potential application in the food safety detection.

scholarly journals A novel electrochemical aptasensor for fumonisin B1 determination using DNA and exonuclease-I as signal amplification strategy

2019 ◽  
Author(s):  
Min Wei ◽  
Fei Zhao ◽  
Shuo Feng ◽  
Huali Jin
Keyword(s):  
Incubation Time ◽  
Electrode Surface ◽  
Signal Amplification ◽  
Limit Of Detection ◽  
Fumonisin B1 ◽  
Electrochemical Aptasensor ◽  
Experimental Conditions ◽  
Exonuclease I ◽  
Double Stranded Dna ◽  
Amplification Strategy

Abstract In this work, using DNA and exonuclease-I (Exo-I) as signal amplification strategy, a novel and facile electrochemical aptasensor was constructed for fumonisin B1 (FB1) detection. The G-rich complementary DNA (cDNA) was immobilized onto the electrode surface. Then, aptamer of FB1 was hybridized with cDNA to form double-stranded DNA. In the absence of FB1, double-stranded DNA and G-rich cDNA on the electrode surface promoted effectively methylene blue (MB) enrichment and amplified the initial electrochemical response. In the presence of FB1, the combination of aptamer and FB1 led to the release of aptamer from the electrode surface and the expose of 3' end of single-stranded cDNA. When Exo-I was added onto the electrode surface, the single-stranded cDNA was degraded in the 3’-5’ direction. The decrease of double-stranded DNA and G-rich cDNA resulted in the less access of MB to the electrode surface, which decreased the electrochemical signal. The experimental conditions including incubation time of FB1, the amount of Exo-I and incubation time of Exo-I were optimized. Under the optimal conditions, the linear relationship between the change of peak current and the logarithmic concentration of FB1 was observed in the range of 1.0×10-3-1000ng·mL−1 with a low limit of detection of 0.15 pg·mL−1. The experimental results showed that the prepared aptasensor had acceptable specificity, reproducibility, repeatability and stability. Therefore, this proposed aptasensor has a potential application in the food safety detection.

scholarly journals A novel electrochemical aptasensor for fumonisin B1 determination using DNA and exonuclease-I as signal amplification strategy

2019 ◽  
Author(s):  
Min Wei ◽  
Fei Zhao ◽  
Shuo Feng ◽  
Huali Jin
Keyword(s):  
Incubation Time ◽  
Electrode Surface ◽  
Signal Amplification ◽  
Limit Of Detection ◽  
Fumonisin B1 ◽  
Electrochemical Aptasensor ◽  
Experimental Conditions ◽  
Exonuclease I ◽  
Double Stranded Dna ◽  
Amplification Strategy

Abstract In this work, using DNA and exonuclease-I (Exo-I) as signal amplification strategy, a novel and facile electrochemical aptasensor was constructed for fumonisin B1 (FB1) detection. The G-rich complementary DNA (cDNA) was immobilized onto the electrode surface. Then, aptamer of FB1 was hybridized with cDNA to form double-stranded DNA. In the absence of FB1, double-stranded DNA and G-rich cDNA on the electrode surface promoted effectively methylene blue (MB) enrichment and amplified the initial electrochemical response. In the presence of FB1, the combination of aptamer and FB1 led to the release of aptamer from the electrode surface and the expose of 3' end of single-stranded cDNA. When Exo-I was added onto the electrode surface, the single-stranded cDNA was degraded in the 3’-5’ direction. The decrease of double-stranded DNA and G-rich cDNA resulted in the less access of MB to the electrode surface, which decreased the electrochemical signal. The experimental conditions including incubation time of FB1, the amount of Exo-I and incubation time of Exo-I were optimized. Under the optimal conditions, the linear relationship between the change of peak current and the logarithmic concentration of FB1 was observed in the range of 1.0×10-3-1000ng·mL−1 with a low limit of detection of 0.15 pg·mL−1. The experimental results showed that the prepared aptasensor had acceptable specificity, reproducibility, repeatability and stability. Therefore, this proposed aptasensor has a potential application in the food safety detection.

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