An electrochemiluminescence biosensor for endonuclease EcoRI detection

2017 ◽  
Vol 89 ◽  
pp. 585-591 ◽  
Author(s):  
Yingjie Li ◽  
Yuqin Li ◽  
Yaoyu Wu ◽  
Fushen Lu ◽  
Yaowen Chen ◽  
...  
Keyword(s):  
Endonuclease Ecori

Characterisation of plasmids coding for the restriction endonuclease EcoRI

1976 ◽  
Vol 143 (3) ◽  
pp. 319-325 ◽  
Author(s):  
H. R. Smith ◽  
G. O. Humphreys ◽  
Geraldine A. Willshaw ◽  
E. S. Anderson
Keyword(s):  
Restriction Endonuclease ◽  
Restriction Endonuclease Ecori ◽  
Endonuclease Ecori

Site-directed mutagenesis in the catalytic center of the restriction endonuclease EcoRI

Gene ◽  
1995 ◽  
Vol 157 (1-2) ◽  
pp. 113-118 ◽  
Author(s):  
Gabriele Grabowski ◽  
Albert Jeltsch ◽  
Heiner Wolfes ◽  
Günter Maass ◽  
Jürgen Alves
Keyword(s):  
Restriction Endonuclease ◽  
Site Directed Mutagenesis ◽  
Directed Mutagenesis ◽  
Catalytic Center ◽  
Restriction Endonuclease Ecori ◽  
Endonuclease Ecori

scholarly journals Analysis of DNA attached to the chromosome scaffold.

1982 ◽  
Vol 93 (2) ◽  
pp. 278-284 ◽  
Author(s):  
M T Kuo
Keyword(s):  
Dna Sequences ◽  
Southern Blotting ◽  
Nuclear Dna ◽  
Micrococcal Nuclease ◽  
Nonhistone Protein ◽  
Sequence Class ◽  
Restriction Endonuclease Ecori ◽  
Cold Spring ◽  
Endonuclease Ecori ◽  
Chromosome Scaffold

Two different methods have been described to investigate whether any specific DNA sequences are intimately associated with the metaphase chromosome scaffold. The chromosome scaffold, prepared by dehistonization of chromosomes with 2 M NaCl, is a nonhistone protein complex to which many looped DNA molecules are attached (Laemmli et al., 1977, Cold Spring Harbor Symp. Quant. Biol. 42:351--360). Chromosome scaffold DNA was prepared from dehistonized chicken MSB chromosomes by restriction endonuclease EcoRI digestion followed by removal of the looped DNA by sucrose gradient sedimentation. Alternatively, the scaffold DNA was prepared from micrococcal nuclease-digested intact chromosomes using sucrose gradients containing 2M NaCl. Solution hybridization of the radioactively labeled scaffold DNA with a large excess of total nuclear DNA revealed that, in either case, the scaffold DNA is not a unique sequence class of genomic DNA. Southern-blotting hybridization also showed that the scaffold DNA prepared from EcoRI-digested dehistonized chromosomes was not enriched (or depleted) in the ovalbumin gene sequences. The possibility of a dynamic interaction of protein and DNA in the chromosome scaffold and the possibility that the scaffold is a preparative artifact are discussed.

Pausing of the Restriction Endonuclease EcoRI during Linear Diffusion on DNA

Biochemistry ◽  
1994 ◽  
Vol 33 (34) ◽  
pp. 10215-10219 ◽  
Author(s):  
Albert Jeltsch ◽  
Juergen Alves ◽  
Heiner Wolfes ◽  
Guenter Maass ◽  
Alfred Pingoud
Keyword(s):  
Restriction Endonuclease ◽  
Linear Diffusion ◽  
Restriction Endonuclease Ecori ◽  
Endonuclease Ecori

Fingerprinting bacterial chromosomal DNA with restriction endonuclease EcoRI: comparison of Rhizobium spp. and identification of mutants

1979 ◽  
Vol 25 (7) ◽  
pp. 803-807 ◽  
Author(s):  
Jonathan R. Mielenz ◽  
L. E. Jackson ◽  
F. O'Gara ◽  
K. T. Shanmugam
Keyword(s):  
Restriction Endonuclease ◽  
Dna Cleavage ◽  
Rhizobium Trifolii ◽  
Chromosomal Dna ◽  
Mutant Strains ◽  
Restriction Endonuclease Ecori ◽  
Endonuclease Ecori ◽  
Cellular Dna ◽  
Derivatives Of ◽  
Japonicum Strain

Total cellular DNA from Rhizobium trifolii, R. meliloti, and R. japonicum strains 110 and 117 were prepared. DNA fragments generated with restriction endonuclease EcoRI from these DNA samples were compared in agarose gels after electrophoresis. DNA cleavage patterns generated from R. japonicum strain 110, R. trifolii, and R. meliloti were clearly distinguishable from each other. Restriction endonuclease cleavage patterns of DNA from R. japonicum strain 110 and presumptive R. trifolii mutant strains that nodulate soybean were found to be similar. Rhizobium trifolii mutant strains were also lysed by a phage specific for R. japonicum strain 110. These results show that "R. trifolii mutant strains" are indeed derivatives of R. japonicum strain 110 and not R. trifolii.

Walnut (Juglans spp.) genetic diversity determined by restriction fragment length polymorphisms

Genome ◽  
1994 ◽  
Vol 37 (4) ◽  
pp. 690-700 ◽  
Author(s):  
R. G. Fjellstrom ◽  
D. E. Parfitt
Keyword(s):  
Genetic Diversity ◽  
East Asian ◽  
Distinct Species ◽  
Western Hemisphere ◽  
Germplasm Preservation ◽  
Asian Populations ◽  
Length Polymorphisms ◽  
Restriction Endonuclease Ecori ◽  
Juglans Hindsii ◽  
Endonuclease Ecori

The genetic diversity of 13 Juglans species was characterized using nuclear RFLPs. Allelic frequencies among 41 Juglans populations were determined at 19 RFLP loci by hybridizing single locus probes to walnut DNAs digested with the restriction endonuclease EcoRI or HindIII. A 10-fold difference in species heterozygosity levels was seen among species in different sections of the genus. Differentiation among conspecific populations varied over threefold between species. Genetic differentiation among conspecific east Asian populations was larger than that seen among east Asian species, while the opposite trend was seen for Western Hemisphere species. Taxonomic affinities were also indicated by these results, suggesting that J. cinerea should be included as part of section Cardiocaryon rather than as a unique section, Trachycaryon. Juglans hindsii is classified as a distinct species and not a subspecies of J. californica. Strategies for germplasm preservation and species requiring marked collection efforts are given.Key words: Juglans, RFLP, genetic diversity, walnut.

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