Development of double-generation gold nanoparticle chip-based dengue virus detection system combining fluorescence turn-on probes

2016 ◽  
Vol 77 ◽  
pp. 90-98 ◽  
Author(s):  
Yen-Ting Tung ◽  
Cheng-Chung Chang ◽  
Yi-Ling Lin ◽  
Shie-Liang Hsieh ◽  
Gou-Jen Wang
2018 ◽  
Vol 22 (2) ◽  
pp. 75
Author(s):  
Budi Saksono

      In the previous paper, we had succeeded in developing an early detection system of dengue viruses using Sugar liganded Gold Nano Particle (SGNP) only from 6 μL serum. It has been reported that dengue virus is also detected in the saliva and urine of the patient. The evidences lead to the possibility of developing non-invasive methods of dengue virus detection. In this in vitro study, we evaluated the utility of SGNP to capture and concentrate dengue virion in 10% saliva solution. The results showed that dengue virion was successfully detected in 10% of saliva solution. Analysis of virion stability during storage showed that virions in salivary samples were stable up to 3 days at temperature wherease the RNA has significantly degraded. Although still a preliminary study, the data obtained show the prospect of SGNP as a non-invasive dengue virus detection method, as well as the development of POC (Point of Care) method. Clinical trials using saliva from dengue viruses infected patients need to be done to prove the effectiveness of the SGNP method.


RSC Advances ◽  
2016 ◽  
Vol 6 (60) ◽  
pp. 54777-54784 ◽  
Author(s):  
Uchangi Satyaprasad Akshath ◽  
Praveena Bhatt

Ultrasensitive detection of formaldehyde by coupling enzyme activity with GNP synthesis.


2015 ◽  
Vol 7 (6) ◽  
pp. 2278-2282 ◽  
Author(s):  
Dan Li ◽  
Biao Li ◽  
Sung Ik Yang

We report on a rapid and selective fluorescence turn-on detection system for Cu2+ based on the formation of highly fluorescent copper nanoclusters (CuNCs).


2013 ◽  
Vol 10 (1) ◽  
Author(s):  
James R Carter ◽  
Velmurugan Balaraman ◽  
Cheryl A Kucharski ◽  
Tresa S Fraser ◽  
Malcolm J Fraser

2018 ◽  
Author(s):  
Suying Xu ◽  
Adam Sedgwick ◽  
Souad Elfecky ◽  
Wenbo Chen ◽  
Ashley Jones ◽  
...  

<p>A boronic acid-based anthracene fluorescent probe was functionalised with an acrylamide unit to incorporate into a hydrogel system for monosaccharide detection<i>. </i>In solution, the fluorescent probe<b> </b>displayed a strong fluorescence turn-on response upon exposure to fructose, and an expected trend in apparent binding constants, as judged by a fluorescence response where D-fructose > D-galactose > D-mannose > D-glucose. The hydrogel incorporating the boronic acid monomer demonstrated the ability to detect monosaccharides by fluorescence with the same overall trend as the monomer in solution with the addition of fructose resulting in a 10-fold enhancement (≤ 0.25 M). <b><u></u></b></p>


2005 ◽  
Author(s):  
Rabih E. Jabbour ◽  
Deborah Kuzmanovic ◽  
Patrick E. McCubbin ◽  
Ilya Elashvili ◽  
Charles H. Wick

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