A novel sensitive cell-based Love Wave biosensor for marine toxin detection

2016 ◽  
Vol 77 ◽  
pp. 573-579 ◽  
Author(s):  
Xi Zhang ◽  
Jiaru Fang ◽  
Ling Zou ◽  
Yingchang Zou ◽  
Lang Lang ◽  
...  
Keyword(s):  
Love Wave ◽  
Sensitive Cell ◽  
Toxin Detection ◽  
Marine Toxin

The problem of toxicity equivalent factors in developing alternative methods to animal bioassays for marine-toxin detection

2010 ◽  
Vol 29 (11) ◽  
pp. 1316-1325 ◽  
Author(s):  
Luis M. Botana ◽  
Natalia Vilariño ◽  
Amparo Alfonso ◽  
Carmen Vale ◽  
Carmen Louzao ◽  
...  
Keyword(s):  
Alternative Methods ◽  
Toxin Detection ◽  
Marine Toxin ◽  
Toxicity Equivalent

Biological methods for marine toxin detection

2010 ◽  
Vol 397 (5) ◽  
pp. 1673-1681 ◽  
Author(s):  
Natalia Vilariño ◽  
M. Carmen Louzao ◽  
Mercedes R. Vieytes ◽  
Luis M. Botana
Keyword(s):  
Toxin Detection ◽  
Marine Toxin ◽  
Biological Methods

Study of permeability of the marine toxin azaspiracid-1 over in vitro human intestinal model

Planta Medica ◽  
2016 ◽  
Vol 81 (S 01) ◽  
pp. S1-S381
Author(s):  
P Abal ◽  
MC Louzao ◽  
M Fraga ◽  
N Vilariño ◽  
S Ferreiro ◽  
...  
Keyword(s):  
Marine Toxin

scholarly journals Primary Cultures of Embryonic Chicken Neurons for Sensitive Cell-Based Assay of Botulinum Neurotoxin: Implications for Therapeutic Discovery

2007 ◽  
Vol 12 (3) ◽  
pp. 370-377 ◽  
Author(s):  
Andrea M. Stahl ◽  
Gordon Ruthel ◽  
Edna Torres-Melendez ◽  
Tara A. Kenny ◽  
Rekha G. Panchal ◽  
...  
Keyword(s):  
Nervous System ◽  
Botulinum Toxin ◽  
Neutralizing Antibodies ◽  
Botulinum Neurotoxin ◽  
Potent Inhibitor ◽  
Primary Cultures ◽  
Embryonic Chick ◽  
Sensitive Cell ◽  
Botulinum Neurotoxin A ◽  
Therapeutic Compounds

Botulinum toxin is an exceedingly potent inhibitor of neurotransmission across the neuromuscular junction, causing flaccid paralysis and death. The potential for misuse of this deadly poison as a bioweapon has added a greater urgency to the search for effective therapeutics. The development of sensitive and efficient cell-based assays for the evaluation of toxin antagonists is crucial to the rapid and successful identification of therapeutic compounds. The authors evaluated the sensitivity of primary cultures from 4 distinct regions of the embryonic chick nervous system to botulinum neurotoxin A (BoNT/A) cleavage of synaptosomal-associated protein of 25 kD (SNAP-25). Although differences in sensitivity were apparent, SNAP-25 cleavage was detectable in neuronal cells from each of the 4 regions within 3 h at BoNT/A concentrations of 1 nM or lower. Co-incubation of chick neurons with BoNT/A and toxin-neutralizing antibodies inhibited SNAP-25 cleavage, demonstrating the utility of these cultures for the assay of BoNT/A antagonists. ( Journal of Biomolecular Screening 2007:370-377)

Conjugated Polymer-Based Photoelectrochemical Cytosensor with Turn-On Enable Signal for Sensitive Cell Detection

2018 ◽  
Vol 10 (7) ◽  
pp. 6618-6623 ◽  
Author(s):  
Shanshan Liu ◽  
Ping He ◽  
Sameer Hussain ◽  
Huan Lu ◽  
Xin Zhou ◽  
...  
Keyword(s):  
Conjugated Polymer ◽  
Cell Detection ◽  
Sensitive Cell ◽  
Turn On

scholarly journals Phytochemical Analysis and In Vitro Effects of Allium fistulosum L. and Allium sativum L. Extracts on Human Normal and Tumor Cell Lines: A Comparative Study

Molecules ◽  
2021 ◽  
Vol 26 (3) ◽  
pp. 574
Author(s):  
Adrian Bogdan Țigu ◽  
Cristian Silviu Moldovan ◽  
Vlad-Alexandru Toma ◽  
Anca Daniela Farcaș ◽  
Augustin Cătălin Moț ◽  
...  
Keyword(s):  
Cell Line ◽  
Allium Sativum ◽  
Allium Fistulosum ◽  
Tumor Cell Lines ◽  
Sensitive Cell ◽  
Sensitive Cell Line ◽  
Welsh Onion ◽  
Normal Cells ◽  
Ic50 Value ◽  
High Concentrations

Allium sativum L. (garlic bulbs) and Allium fistulosum L. (Welsh onion leaves) showed quantitative differences of identified compounds: allicin and alliin (380 µg/mL and 1410 µg/mL in garlic; 20 µg/mL and 145 µg/mL in Welsh onion), and the phenolic compounds (chlorogenic acid, p-coumaric acid, ferulic acid, gentisic acid, 4-hydroxybenzoic acid, kaempferol, isoquercitrin, quercitrin, quercetin, and rutin). The chemical composition determined the inhibitory activity of Allium extracts in a dose-dependent manner, on human normal cells (BJ-IC50 0.8841% garlic/0.2433% Welsh onion and HaCaT-IC50 1.086% garlic/0.6197% Welsh onion) and tumor cells (DLD-1-IC50 5.482%/2.124%; MDA-MB-231-IC50 6.375%/2.464%; MCF-7-IC50 6.131%/3.353%; and SK-MES-1-IC50 4.651%/5.819%). At high concentrations, the cytotoxic activity of each extract, on normal cells, was confirmed by: the 50% of the growth inhibition concentration (IC50) value, the cell death induced by necrosis, and biochemical determination of LDH, catalase, and Caspase-3. The four tumor cell lines treated with high concentrations (10%, 5%, 2.5%, and 1.25%) of garlic extract showed different sensibility, appreciated on the base of IC50 value for the most sensitive cell line (SK-MES-1), and the less sensitive (MDA-MB-231) cell line. The high concentrations of Welsh onion extract (5%, 2.5%, and 1.25%) induced pH changes in the culture medium and SK-MES-1 being the less sensitive cell line.

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