Highly sensitive and specific colorimetric detection of cancer cells via dual-aptamer target binding strategy

2015 ◽  
Vol 73 ◽  
pp. 1-6 ◽  
Author(s):  
Kun Wang ◽  
Daoqing Fan ◽  
Yaqing Liu ◽  
Erkang Wang
Keyword(s):  
Cancer Cells ◽  
Colorimetric Detection ◽  
Highly Sensitive ◽  
Target Binding

scholarly journals Fer and FerT Govern Mitochondrial Susceptibility to Metformin and Hypoxic Stress in Colon and Lung Carcinoma Cells

Cells ◽  
2021 ◽  
Vol 10 (1) ◽  
pp. 97
Author(s):  
Odeya Marciano ◽  
Linoy Mehazri ◽  
Sally Shpungin ◽  
Alexander Varvak ◽  
Eldad Zacksenhaus ◽  
...  
Keyword(s):  
Cancer Cells ◽  
Aerobic Glycolysis ◽  
Metastatic Cancer ◽  
Carcinoma Cells ◽  
Metabolic Adaptation ◽  
Hypoxic Stress ◽  
Lung Carcinoma Cells ◽  
Metabolic Role ◽  
Highly Sensitive ◽  
Anticancer Treatment

Aerobic glycolysis is an important metabolic adaptation of cancer cells. However, there is growing evidence that reprogrammed mitochondria also play an important metabolic role in metastatic dissemination. Two constituents of the reprogrammed mitochondria of cancer cells are the intracellular tyrosine kinase Fer and its cancer- and sperm-specific variant, FerT. Here, we show that Fer and FerT control mitochondrial susceptibility to therapeutic and hypoxic stress in metastatic colon (SW620) and non-small cell lung cancer (NSCLC-H1299) cells. Fer- and FerT-deficient SW620 and H1299 cells (SW∆Fer/FerT and H∆Fer/FerT cells, respectively) become highly sensitive to metformin treatment and to hypoxia under glucose-restrictive conditions. Metformin impaired mitochondrial functioning that was accompanied by ATP deficiency and robust death in SW∆Fer/FerT and H∆Fer/FerT cells compared to the parental SW620 and H1299 cells. Notably, selective knockout of the fer gene without affecting FerT expression reduced sensitivity to metformin and hypoxia seen in SW∆Fer/FerT cells. Thus, Fer and FerT modulate the mitochondrial susceptibility of metastatic cancer cells to hypoxia and metformin. Targeting Fer/FerT may therefore provide a novel anticancer treatment by efficient, selective, and more versatile disruption of mitochondrial function in malignant cells.

scholarly journals Immobilization of Pseudomonas aeruginosa static biomass on eggshell powder for on-line preconcentration and determination of Cr (VI)

2020 ◽  
Vol 18 (1) ◽  
pp. 303-313 ◽  
Author(s):  
Aamir Rasheed ◽  
Tahseen Ghous ◽  
Sumaira Mumtaz ◽  
Muhammad Nadeem Zafar ◽  
Kalsoom Akhter ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Low Cost ◽  
Colorimetric Detection ◽  
Time Flow ◽  
Highly Sensitive ◽  
Glass Column ◽  
On Line ◽  
Preconcentration Time ◽  
Ultra Trace

AbstractIn the present work, a novel continuous flow system (CFS) is developed for the preconcentration and determination of Cr (VI) using Pseudomonas aeruginosa static biomass immobilized onto an effective and low-cost solid support of powdered eggshells. A mini glass column packed with the immobilized biosorbent is incorporated in a CFS for the preconcentration and determination of Cr (VI) from aqueous solutions. The method is based on preconcentration, washing and elution steps followed by colorimetric detection with 1,5-diphenyl carbazide in sulphuric acid. The effects of several variables such as pH, retention time, flow rate, eluent concentration and loaded volume are studied. Under optimal conditions, the CFS method has a linear range between 10 and 100 μg L-1 and a detection limit of 6.25 μg L-1 for the determination of Cr (VI). The sampling frequency is 10 samples per hour with a preconcentration time of 5 mins. Furthermore, after washing with a 0.1 M buffer (pH 3.0), the activity of the biosorbent is regenerated and remained comparable for more than 200 cycles. Scanning electron microscopy reveals a successful immobilization of biomass on eggshells powder and precipitation of Cr (VI) on the bacterial cell surface. The proposed method proves highly sensitive and could be suitable for the determination of Cr (VI) at an ultra-trace level.

An anthracene–quinoline based dual-mode fluorometric–colorimetric sensor for the detection of Fe3+ and its application in live cell imaging

2021 ◽  
Author(s):  
Tahereh Tehrani ◽  
Soraia Meghdadi ◽  
Zohreh Salarvand ◽  
Behnam Tavakoli ◽  
Kiamars Eskandari ◽  
...  
Keyword(s):  
Live Cell Imaging ◽  
Cell Imaging ◽  
Colorimetric Detection ◽  
Colorimetric Sensor ◽  
Live Cell ◽  
Dual Mode ◽  
Highly Sensitive

A highly sensitive anthracene–quinoline based dual-mode sensor has been synthesized and used for the fluorometric and colorimetric detection of Fe3+ and in live cell imaging.

scholarly journals Highly sensitive and selective colorimetric detection of dual metal ions (Hg2+ and Sn2+) in water: an eco-friendly approach

RSC Advances ◽  
2021 ◽  
Vol 11 (24) ◽  
pp. 14700-14709
Author(s):  
Rintumoni Paw ◽  
Moushumi Hazarika ◽  
Purna K. Boruah ◽  
Amlan Jyoti Kalita ◽  
Ankur K. Guha ◽  
...  
Keyword(s):  
Metal Ions ◽  
Ag Nanoparticles ◽  
Limit Of Detection ◽  
Colorimetric Detection ◽  
Garlic Extract ◽  
Selective Detection ◽  
Highly Sensitive ◽  
Dual Metal

Synthesis of Ag nanoparticles using Allin based garlic extract for highly sensitive and selective detection of metal ions Hg2+ and Sn2+ in water. The limit of detection (LoD) for Hg2+ and Sn2+ ions were found as 15.7 nM and 11.25 nM respectively.

scholarly journals Highly sensitive colorimetric detection of 17β-estradiol using split DNA aptamers immobilized on unmodified gold nanoparticles

2014 ◽  
Vol 4 (1) ◽  
Author(s):  
Jinchuan Liu ◽  
Wenhui Bai ◽  
Shucao Niu ◽  
Chao Zhu ◽  
Shuming Yang ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Colorimetric Detection ◽  
Dna Aptamers ◽  
Highly Sensitive ◽  
17Β Estradiol

scholarly journals Selective and Irreversible Induction of Necroptotic Cell Death in Lung Tumorspheres by Short-Term Exposure to Verapamil in Combination with Sorafenib

2017 ◽  
Vol 2017 ◽  
pp. 1-9 ◽  
Author(s):  
Juan Sebastian Yakisich ◽  
Yogesh Kulkarni ◽  
Neelam Azad ◽  
Anand Krishnan V. Iyer
Keyword(s):  
Cancer Cells ◽  
Culture Conditions ◽  
Serum Starvation ◽  
Adherent Cells ◽  
Key Factors ◽  
Short Term ◽  
Short Term Exposure ◽  
Highly Sensitive ◽  
Free Media ◽  
Drug Free

The presence of highly resistant cancer cells and the toxicity to normal cells are key factors that limit chemotherapy. Here, we used two models of highly resistant lung cancer cells: (1) adherent cells growing under prolonged periods of serum starvation (PPSS) and (2) cells growing as floating tumorspheres (FTs) to evaluate the effect of Verapamil (VP) in combination with Sorafenib (SF). Compared to cells growing under routine culture conditions (RCCs), PPPS cells or FTs were highly sensitive to short-term exposure (24 h) to VP 100 μM + SF 5 μM (VP100 + SF5). Recovery experiments exposing cells to VP100 + SF5 for 24 h followed by incubation in drug-free media for 48 h demonstrated that while PPSS as well as FT cells were unable to recover, cancer cells and the noncancerous cell line Beas-2B growing under RCCs were less sensitive and were also able to recover significantly. VP100 + SF5 induced significant changes in the expression of protein associated with apoptosis, autophagy, and to a lesser extent necroptosis. Coincubation experiments with z-VAD-FMK, necrostatin 1, or chloroquine showed evidence that necroptosis played a central role. Our data demonstrates that highly resistant cancer cells can be selectively eliminated by VP + SF and that necroptosis plays a central role.

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