Ultrasensitive electrochemical immunosensor for carbohydrate antigen 72-4 based on dual signal amplification strategy of nanoporous gold and polyaniline–Au asymmetric multicomponent nanoparticles

2015 ◽  
Vol 64 ◽  
pp. 51-56 ◽  
Author(s):  
Haixia Fan ◽  
Zhankui Guo ◽  
Liang Gao ◽  
Yong Zhang ◽  
Dawei Fan ◽  
...  
Keyword(s):  
Signal Amplification ◽  
Carbohydrate Antigen ◽  
Electrochemical Immunosensor ◽  
Nanoporous Gold ◽  
Amplification Strategy ◽  
Dual Signal Amplification

Dual signal amplification strategy for electrochemical detection of platelet-derived growth factor BB

2017 ◽  
Vol 9 (46) ◽  
pp. 6569-6573 ◽  
Author(s):  
Bin Xie ◽  
Ning Zhou ◽  
Rong Ding ◽  
Yuanchao Zhao ◽  
Bohan Zhang ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Growth Factor ◽  
Electrochemical Detection ◽  
Signal Amplification ◽  
Electrochemical Immunosensor ◽  
Platelet Derived Growth Factor ◽  
Amplification Strategy ◽  
Dual Signal Amplification

An electrochemical immunosensor for the platelet-derived growth factor BB (PDGF-BB) is reported based on dual signal amplification of aptamers and alkaline phosphatase.

scholarly journals MiRNA Detection Using a Rolling Circle Amplification and RNA-Cutting Allosteric Deoxyribozyme Dual Signal Amplification Strategy

Biosensors ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 222
Author(s):  
Chenxin Fang ◽  
Ping Ouyang ◽  
Yuxing Yang ◽  
Yang Qing ◽  
Jialun Han ◽  
...  
Keyword(s):  
Signal Amplification ◽  
Rolling Circle Amplification ◽  
Padlock Probe ◽  
Rolling Circle ◽  
Sensing Platform ◽  
Mirna Detection ◽  
Substrate Cleavage ◽  
Amplification Strategy ◽  
Circular Template ◽  
Dual Signal Amplification

A microRNA (miRNA) detection platform composed of a rolling circle amplification (RCA) system and an allosteric deoxyribozyme system is proposed, which can detect miRNA-21 rapidly and efficiently. Padlock probe hybridization with the target miRNA is achieved through complementary base pairing and the padlock probe forms a closed circular template under the action of ligase; this circular template results in RCA. In the presence of DNA polymerase, RCA proceeds and a long chain with numerous repeating units is formed. In the presence of single-stranded DNA (H1 and H2), multi-component nucleic acid enzymes (MNAzymes) are formed that have the ability to cleave substrates. Finally, substrates containing fluorescent and quenching groups and magnesium ions are added to the system to activate the MNAzyme and the substrate cleavage reaction, thus achieving fluorescence intensity amplification. The RCA–MNAzyme system has dual signal amplification and presents a sensing platform that demonstrates broad prospects in the analysis and detection of nucleic acids.

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