Gold nanoparticle enhanced fluorescence anisotropy for the assay of single nucleotide polymorphisms (SNPs) based on toehold-mediated strand-displacement reaction

2013 ◽  
Vol 41 ◽  
pp. 569-575 ◽  
Author(s):  
Xinyi Wang ◽  
Mingjian Zou ◽  
Hongduan Huang ◽  
Yuqian Ren ◽  
Limei Li ◽  
...  
Keyword(s):  
Single Nucleotide Polymorphisms ◽  
Gold Nanoparticle ◽  
Fluorescence Anisotropy ◽  
Displacement Reaction ◽  
Nucleotide Polymorphisms ◽  
Strand Displacement ◽  
Enhanced Fluorescence ◽  
Single Nucleotide ◽  
Strand Displacement Reaction

scholarly journals Homogeneous Real-Time Detection of Single-Nucleotide Polymorphisms by Strand Displacement Amplification on the BD ProbeTec ET System

2003 ◽  
Vol 49 (10) ◽  
pp. 1599-1607 ◽  
Author(s):  
Sha-Sha Wang ◽  
Keith Thornton ◽  
Andrew M Kuhn ◽  
James G Nadeau ◽  
Tobin J Hellyer
Keyword(s):  
Single Nucleotide Polymorphisms ◽  
Dna Sequencing ◽  
Real Time ◽  
Whole Blood ◽  
Nucleotide Polymorphisms ◽  
Strand Displacement ◽  
Strand Displacement Amplification ◽  
Single Nucleotide ◽  
Buccal Swabs ◽  
Real Time Detection

Abstract Background: The BD ProbeTec™ ET System is based on isothermal strand displacement amplification (SDA) of target nucleic acid coupled with homogeneous real-time detection using fluorescent probes. We have developed a novel, rapid method using this platform that incorporates a universal detection format for identification of single-nucleotide polymorphisms (SNPs) and other genotypic variations. Method: The system uses a common pair of fluorescent Detector Probes in conjunction with unlabeled allele-specific Adapter Primers and a universal buffer chemistry to permit analysis of multiple SNP loci under generic assay conditions. We used Detector Probes labeled with different dyes to facilitate differentiation of two alternative alleles in a single reaction with no postamplification manipulation. We analyzed six SNPs within the human β2-adrenergic receptor (β2AR) gene, using whole blood, buccal swabs, and urine samples, and compared results with those obtained by DNA sequencing. Results: Unprocessed whole blood was successfully genotyped with as little as 0.1–1 μL of sample per reaction. All six β2AR assays were able to accommodate ≥20 μL of unprocessed whole blood. For the 14 individuals tested, genotypes determined with the six β2AR assays agreed with DNA sequencing results. Conclusion: SDA-based allelic differentiation on the BD ProbeTec ET System can detect SNPs rapidly, using whole blood, buccal swabs, or urine.

Target Induced-DNA strand displacement reaction using gold nanoparticle labeling for hepatitis E virus detection

2020 ◽  
Vol 1134 ◽  
pp. 10-17
Author(s):  
Tatchanun Ngamdee ◽  
Lee Su Yin ◽  
Sompong Vongpunsawad ◽  
Yong Poovorawan ◽  
Werasak Surareungchai ◽  
...  
Keyword(s):  
Gold Nanoparticle ◽  
Hepatitis E Virus ◽  
Virus Detection ◽  
Hepatitis E ◽  
Displacement Reaction ◽  
Strand Displacement ◽  
Dna Strand Displacement ◽  
Dna Strand ◽  
Strand Displacement Reaction

Reusable Electrochemical Biosensor for Sensitive Detection of Single Nucleotide Polymorphisms Based on Gold Nanoparticle Self-Assembly

2014 ◽  
Vol 651-653 ◽  
pp. 293-296
Author(s):  
Song Bai Zhang ◽  
Chun Jiao Tang ◽  
Liao Yong Luo ◽  
Na Liu ◽  
Qin Li Sun ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Single Nucleotide Polymorphisms ◽  
Gold Nanoparticle ◽  
Self Assembly ◽  
Gold Electrode ◽  
Sensitive Detection ◽  
Capture Probe ◽  
Nucleotide Polymorphisms ◽  
Single Nucleotide ◽  
Target Dna

A reusable electrochemical biosensing strategy based on gold nanoparticle involved layer-by-layer self-assembly for sensitive detection of single nucleotide polymorphisms is proposed in this study. Making use of the strong sulfur-Au affinity, ethanthiol and capture probe modified gold nanoparticles are self-assembled onto the surface of gold electrode successively. The target DNA hybridizes with the capture probe and ferrocene labeled signaling probe successively via sandwich hybridization reaction. By measuring ac current voltammetry, the target DNA can be sensitively detected in a linear dynamic range from 4.1-410 nM with a low detection limit of 2 nM. Making use of self-assembled gold nanoparticles layer, a large amount of capture probes can be modified onto the gold electrode, supporting the high sensitivity of the proposed strategy. In addition, good reproducibility, high selectivity and stability are achieved. In particular, the biosensor can be easily regenerated by melting in hot water, making it reusable.

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