Tubular waveguide evanescent field absorption biosensor based on particle plasmon resonance for multiplex label-free detection

2013 ◽  
Vol 41 ◽  
pp. 268-274 ◽  
Author(s):  
Hsing-Ying Lin ◽  
Chen-Han Huang ◽  
Shin-Huei Chen ◽  
Yu-Chia Liu ◽  
Wei-Zhe Chang ◽  
...  
Keyword(s):  
Plasmon Resonance ◽  
Evanescent Field ◽  
Label Free ◽  
Label Free Detection ◽  
Particle Plasmon

scholarly journals Fiber Optic Particle Plasmon Resonance Biosensor for Label-Free Detection of Nucleic Acids and Its Application to HLA-B27 mRNA Detection in Patients with Ankylosing Spondylitis

Sensors ◽  
2020 ◽  
Vol 20 (11) ◽  
pp. 3137
Author(s):  
Yen-Ta Tseng ◽  
Wan-Yun Li ◽  
Ya-Wen Yu ◽  
Chang-Yue Chiang ◽  
Su-Qin Liu ◽  
...  
Keyword(s):  
Ankylosing Spondylitis ◽  
Nucleic Acids ◽  
Real Time ◽  
Plasmon Resonance ◽  
Fiber Optic ◽  
Hla B27 ◽  
Label Free ◽  
Mrna Detection ◽  
Label Free Detection ◽  
Particle Plasmon

We developed a label-free, real-time, and highly sensitive nucleic acid biosensor based on fiber optic particle plasmon resonance (FOPPR). The biosensor employs a single-strand deoxyoligonucleotides (ssDNA) probe, conjugated to immobilized gold nanoparticles on the core surface of an optical fiber. We explore the steric effects on hybridization affinity and limit of detection (LOD), by using different ssDNA probe designs and surface chemistries, including diluent molecules of different lengths in mixed self-assembled monolayers, ssDNA probes of different oligonucleotide lengths, ssDNA probes in different orientations to accommodate target oligonucleotides with a hybridization region located unevenly in the strand. Based on the optimized ssDNA probe design and surface chemistry, we achieved LOD at sub-nM level, which makes detection of target oligonucleotides as low as 1 fmol possible in the 10-μL sensor chip. Additionally, the FOPPR biosensor shows a good correlation in determining HLA-B27 mRNA, in extracted blood samples from patients with ankylosing spondylitis (AS), with the clinically accepted real-time reverse transcription-polymerase chain reaction (RT-PCR) method. The results from this fundamental study should guide the design of ssDNA probe for anti-sense sensing. Further results through application to HLA-B27 mRNA detection illustrate the feasibility in detecting various nucleic acids of chemical and biological relevance.

scholarly journals Trends in SPR Cytometry: Advances in Label-Free Detection of Cell Parameters

Biosensors ◽  
2018 ◽  
Vol 8 (4) ◽  
pp. 102 ◽  
Author(s):  
Richard Schasfoort ◽  
Fikri Abali ◽  
Ivan Stojanovic ◽  
Gestur Vidarsson ◽  
Leon Terstappen
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Mammalian Cells ◽  
Living Cells ◽  
Label Free ◽  
Intact Cells ◽  
Cell Parameters ◽  
Spr Imaging ◽  
Label Free Detection

SPR cytometry entails the measurement of parameters from intact cells using the surface plasmon resonance (SPR) phenomenon. Specific real-time and label-free binding of living cells to sensor surfaces has been made possible through the availability of SPR imaging (SPRi) instruments and researchers have started to explore its potential in the last decade. Here we will discuss the mechanisms of detection and additionally describe the problems and issues of mammalian cells in SPR biosensing, both from our own experience and with information from the literature. Finally, we build on the knowledge and applications that has already materialized in this field to give a forecast of some exciting applications for SPRi cytometry.

Label-Free Detection of Nappa: Surface Plasmon Resonance

2019 ◽  
pp. 287-304
Author(s):  
Manuel Fuentes ◽  
Sanjeeva Svrivastava ◽  
Nirosahan Ramachandran ◽  
Eugenie Hainsworth ◽  
Josh LaBaer
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Label Free ◽  
Label Free Detection

scholarly journals Surface Plasmon Resonance Biosensor for Rapid Label-Free Detection of Microribonucleic Acid at Subfemtomole Level

2010 ◽  
Vol 82 (24) ◽  
pp. 10110-10115 ◽  
Author(s):  
Hana Šípová ◽  
Shile Zhang ◽  
Aimée M. Dudley ◽  
David Galas ◽  
Kai Wang ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Label Free ◽  
Surface Plasmon Resonance Biosensor ◽  
Label Free Detection
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