Effects of diamond-FET-based RNA aptamer sensing for detection of real sample of HIV-1 Tat protein

2013 ◽  
Vol 40 (1) ◽  
pp. 277-282 ◽  
Author(s):  
A. Rahim Ruslinda ◽  
Kyosuke Tanabe ◽  
Shoji Ibori ◽  
Xianfen Wang ◽  
Hiroshi Kawarada
Keyword(s):  
Real Sample ◽  
Rna Aptamer ◽  
Tat Protein ◽  
Hiv 1

Development of an RNA aptamer that acquires binding capacity against HIV-1 Tat protein via G-quadruplex formation in response to potassium ions

2017 ◽  
Vol 53 (52) ◽  
pp. 7056-7059 ◽  
Author(s):  
Yudai Yamaoki ◽  
Takashi Nagata ◽  
Tsukasa Mashima ◽  
Masato Katahira
Keyword(s):  
Binding Capacity ◽  
Binding Activity ◽  
Potassium Ions ◽  
Rna Aptamer ◽  
Tat Protein ◽  
G Quadruplex ◽  
Quadruplex Formation ◽  
Target Binding ◽  
Hiv 1

The development of the first K+-responsive RNA aptamer demonstrating ON/OFF switching of its target-binding activity by sensing the addition/removal of K+ is reported.

HIV-1 tat protein expression in mouse brain potentiates ethanol reward and reinstates extinguished ethanol-seeking behavior

2014 ◽  
Vol 140 ◽  
pp. e141-e142
Author(s):  
Jay P. McLaughlin ◽  
M.L. Ganno ◽  
S.O. Eans ◽  
Jason J. Paris ◽  
H.D. Singh
Keyword(s):  
Protein Expression ◽  
Mouse Brain ◽  
Tat Protein ◽  
Seeking Behavior ◽  
Hiv 1

scholarly journals Identification of a novel cell attachment domain in the HIV-1 Tat protein and its 90-kDa cell surface binding protein.

1993 ◽  
Vol 268 (7) ◽  
pp. 5279-5284
Author(s):  
B.S. Weeks ◽  
K. Desai ◽  
P.M. Loewenstein ◽  
M.E. Klotman ◽  
P.E. Klotman ◽  
...  
Keyword(s):  
Cell Surface ◽  
Cell Attachment ◽  
Binding Protein ◽  
Tat Protein ◽  
Surface Binding ◽  
Cell Surface Binding ◽  
Hiv 1

scholarly journals Transduction of Cu,Zn-superoxide dismutase mediated by an HIV-1 Tat protein basic domain into mammalian cells

FEBS Letters ◽  
2000 ◽  
Vol 485 (2-3) ◽  
pp. 163-167 ◽  
Author(s):  
Hyeok Yil Kwon ◽  
Won Sik Eum ◽  
Hyun Woo Jang ◽  
Jung Hoon Kang ◽  
Jiyoon Ryu ◽  
...  
Keyword(s):  
Superoxide Dismutase ◽  
Mammalian Cells ◽  
Tat Protein ◽  
Basic Domain ◽  
Hiv 1

scholarly journals The Human Immunodeficiency Virus Type 1 Tat Protein Up-Regulates the Promoter Activity of the Beta-Chemokine Monocyte Chemoattractant Protein 1 in the Human Astrocytoma Cell Line U-87 MG: Role of SP-1, AP-1, and NF-κB Consensus Sites

2000 ◽  
Vol 74 (4) ◽  
pp. 1632-1640 ◽  
Author(s):  
Siew Pheng Lim ◽  
Alfredo Garzino-Demo
Keyword(s):  
Human Immunodeficiency Virus ◽  
Promoter Activity ◽  
Tat Protein ◽  
Monocyte Chemoattractant Protein 1 ◽  
Monocyte Chemoattractant ◽  
Monocyte Chemoattractant Protein ◽  
Astrocytoma Cell ◽  
Immunodeficiency Virus ◽  
Hiv 1

ABSTRACT It has been shown that the human immunodeficiency virus type 1 (HIV-1) Tat protein can specifically enhance expression and release of monocyte chemoattractant protein 1 (MCP-1) from human astrocytes. In this study, we show evidence that Tat-induced MCP-1 expression is mediated at the transcriptional level. Transient transfection of an expression construct encoding the full-length Tat into the human glioblastoma-astrocytoma cell line U-87 MG enhances reporter gene activity from cotransfected deletion constructs of the MCP-1 promoter. HIV-1 Tat exerts its effect through a minimal construct containing 213 nucleotides upstream of the translational start site. Site-directed mutagenesis studies indicate that an SP1 site (located between nucleotides −123 and −115) is critical for both constitutive and Tat-enhanced expression of the human MCP-1 promoter, as mutation of this SP1 site significantly diminished reporter gene expression in both instances. Gel retardation experiments further demonstrate that Tat strongly enhances the binding of SP1 protein to its DNA element on the MCP-1 promoter. Moreover, we also observe an increase in the binding activities of transcriptional factors AP1 and NF-κB to the MCP-1 promoter following Tat treatment. Mutagenesis studies show that an upstream AP1 site and an adjacent NF-κB site (located at −128 to −122 and −150 to −137, respectively) play a role in Tat-mediated transactivation. In contrast, a further upstream AP1 site (−156 to −150) does not appear to be crucial for promoter activity. We postulate that a Tat-mediated increase in SP1 binding activities augments the binding of AP1 and NF-κB, leading to synergistic activation of the MCP-1 promoter.

Fragments of the HIV-1 Tat protein specifically bind TAR RNA

Science ◽  
1990 ◽  
Vol 249 (4974) ◽  
pp. 1281-1285 ◽  
Author(s):  
K. Weeks ◽  
C Ampe ◽  
S. Schultz ◽  
T. Steitz ◽  
D. Crothers
Keyword(s):  
Tat Protein ◽  
Tar Rna ◽  
Hiv 1

scholarly journals Protein Transduction Domain of HIV-1 Tat Protein Promotes Efficient Delivery of DNA into Mammalian Cells

2001 ◽  
Vol 276 (28) ◽  
pp. 26204-26210 ◽  
Author(s):  
Akiko Eguchi ◽  
Teruo Akuta ◽  
Hajime Okuyama ◽  
Takao Senda ◽  
Haruhiko Yokoi ◽  
...  
Keyword(s):  
Mammalian Cells ◽  
Protein Transduction ◽  
Protein Transduction Domain ◽  
Tat Protein ◽  
Efficient Delivery ◽  
Hiv 1

scholarly journals HIV-1 Tat protein induces DNA damage in human peripheral blood B-lymphocytes via mitochondrial ROS production

Redox Biology ◽  
2018 ◽  
Vol 15 ◽  
pp. 97-108 ◽  
Author(s):  
Rawan El-Amine ◽  
Diego Germini ◽  
Vlada V. Zakharova ◽  
Tatyana Tsfasman ◽  
Eugene V. Sheval ◽  
...  
Keyword(s):  
Dna Damage ◽  
B Lymphocytes ◽  
Peripheral Blood ◽  
Human Peripheral Blood ◽  
Ros Production ◽  
Tat Protein ◽  
Mitochondrial Ros ◽  
Hiv 1
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