scholarly journals Enhanced lateral flow immunoassay using gold nanoparticles loaded with enzymes

2013 ◽  
Vol 40 (1) ◽  
pp. 412-416 ◽  
Author(s):  
Claudio Parolo ◽  
Alfredo de la Escosura-Muñiz ◽  
Arben Merkoçi
Keyword(s):  
Gold Nanoparticles ◽  
Lateral Flow ◽  
Lateral Flow Immunoassay

Lateral Flow Immunoassay Based on Polydopamine-Coated Gold Nanoparticles for the Sensitive Detection of Zearalenone in Maize

2019 ◽  
Vol 11 (34) ◽  
pp. 31283-31290 ◽  
Author(s):  
Shaolan Xu ◽  
Ganggang Zhang ◽  
Bolong Fang ◽  
Qirong Xiong ◽  
Hongwei Duan ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Lateral Flow ◽  
Sensitive Detection ◽  
Lateral Flow Immunoassay

Development of Silver-Coated Gold Nanoparticles and its Conjugation for Labeling on Lateral Flow Immunoassay

2014 ◽  
Vol 1024 ◽  
pp. 273-276
Author(s):  
Siti Rabizah Makhsin ◽  
Khairunisak Abdul Razak ◽  
Rahmah Noordin
Keyword(s):  
Gold Nanoparticles ◽  
Growth Stage ◽  
Silver Ions ◽  
Biological Properties ◽  
Lateral Flow ◽  
Synthesis Process ◽  
Lateral Flow Immunoassay ◽  
Minimum Concentration ◽  
Colloidal Form ◽  
Washing Process

This work describes the formation of silver-coated gold nanoparticles (Ag-AuNPs) using the seeding-growth method. 15 nm AuNPs seeds were synthesized using the citrate reduction method. In the growth stage, the adding sequence of seeds, ascorbic acid (AA), gold chloride (HAuCl4) and silver nitrate (AgNO3) was explored. The effect AgNO3volume (20 mM) was varied at 0.10, 0.25, 0.50, 1.00, 1.25 and 1.50 ml. Morphology of Ag-AuNPs was observed using a Transmission Electron Microscope (TEM) while UV-Vis Spectrophotometer was used to study the concentration and absorption spectra of colloidal Ag-AuNPs. Zeta-sizer analysis was used to study the particle size distribution of Ag-AuNPs in colloidal form. In the growth stage, the optimum adding sequence was found by adding AA as a reducing agent into AuNPs seeds followed by the addition of HAuCl4and AgNO3. This reshuffle sequence is chosen due to the presence of two absorbance peaks at 398 nm and 501 nm compared to others. From TEM images, increasing concentration of silver ions affected the optical properties, end size of Ag-AuNPs as well as raises the concentration of the colloids suspension. The biological properties of samples Ag-AuNPs (0.1 ml to 0.5 ml AgNO3) were studied by performing the conjugation process between samples and antibody; goat anti-human IgA (GaHIgA). An additional washing process was required to perform conjugation for Ag-AuNPs in order to remove by-product or unreacted chemical produced during synthesis process. The Ag-AuNPs was successfully conjugated with GaHIgA and required a minimum concentration of antibody (9µg/ml) after washing process. In addition, the washed Ag-AuNPs also improved its binding capability with GaHIgA by giving higher intense signal when tested with lined Human IgA on the lateral flow immunoassay (LFI) compared to un-washed Ag-AuNPs.

A bare-eye-based lateral flow immunoassay based on the use of gold nanoparticles for simultaneous detection of three pesticides

2014 ◽  
Vol 181 (13-14) ◽  
pp. 1565-1572 ◽  
Author(s):  
Limin Wang ◽  
Jia Cai ◽  
Yulong Wang ◽  
Qingkui Fang ◽  
Suyan Wang ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Simultaneous Detection ◽  
Lateral Flow ◽  
Lateral Flow Immunoassay

scholarly journals Gold Nanobeads with Enhanced Absorbance for Improved Sensitivity in Competitive Lateral Flow Immunoassays

Foods ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 1488
Author(s):  
Xirui Chen ◽  
Xintao Miao ◽  
Tongtong Ma ◽  
Yuankui Leng ◽  
Liangwen Hao ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Detection Limit ◽  
Inhibitory Concentration ◽  
Fumonisin B1 ◽  
Lateral Flow ◽  
Detection Sensitivity ◽  
Lateral Flow Immunoassay ◽  
Promising Strategy ◽  
Qualitative Detection ◽  
Better Than

Background: Colloidal gold based lateral flow immunoassay (LFIA) commonly suffers from relatively low detection sensitivity due to the insufficient brightness of conventional gold nanoparticles (AuNPs) with the size of 20–40 nm. Methods: Herein, three kinds of gold nanobeads (GNBs) with the size of 94 nm, 129 nm, and 237 nm, were synthesized by encapsulating numerous hydrophobic AuNPs (10 nm) into polymer matrix. The synthesized GNBs exhibited the enhanced colorimetric signal intensity compared with 20–40 nm AuNPs. The effects of the size of GNBs on the sensitivity of LFIA with competitive format were assessed. Results: The results showed that the LFIA using 129 nm GNBs as amplified signal probes exhibits the best sensitivity for fumonisin B1 (FB1) detection with a cut-off limit (for visual qualitative detection) at 125 ng/mL, a half maximal inhibitory concentration at 11.27 ng/mL, and a detection limit at 1.76 ng/mL for detection of real corn samples, which are 8-, 3.82-, and 2.89-fold better than those of conventional AuNP40-based LFIA, respectively. The developed GNB-LFIA exhibited negligible cross-reactions with other common mycotoxins. In addition, the accuracy, precision, reliability, and practicability were demonstrated by determining real corn samples. Conclusions: All in all, the proposed study provides a promising strategy to enhance the sensitivity of competitive LFIA via using the GNBs as amplified signal probes.

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