Electrochemical determination of microRNA-21 based on graphene, LNA integrated molecular beacon, AuNPs and biotin multifunctional bio bar codes and enzymatic assay system

2012 ◽  
Vol 33 (1) ◽  
pp. 247-253 ◽  
Author(s):  
Huanshun Yin ◽  
Yunlei Zhou ◽  
Haixia Zhang ◽  
Xiaomeng Meng ◽  
Shiyun Ai
Keyword(s):  
Molecular Beacon ◽  
Electrochemical Determination ◽  
Enzymatic Assay ◽  
Assay System ◽  
Bar Codes

Enzymatic determination of D-alanine with L-alanine dehydrogenase and alanine racemase

2021 ◽  
Author(s):  
Hiroyuki Ashida ◽  
Yoshihiro Sawa ◽  
Tohru Yoshimura
Keyword(s):  
Water Soluble ◽  
Enzymatic Assay ◽  
Assay System ◽  
Alanine Racemase ◽  
Alanine Dehydrogenase ◽  
Enzymatic Determination

Abstract An enzymatic assay system of D-Ala, which is reported to affect the taste, was constructed using alanine racemase and L-alanine dehydrogenase. D-Ala is converted to L-Ala by alanine racemase and then deaminated by L-alanine dehydrogenase with the reduction of NAD+ to NADH, which is determined with water-soluble tetrazolium. Using the assay system, the D-Ala contents of seven crustaceans were determined.

Quantitative Determination of Plasminogen

1970 ◽  
Vol 23 (02) ◽  
pp. 191-201 ◽  
Author(s):  
H. D Bruhn ◽  
L Müller ◽  
F Duckert
Keyword(s):  
Quantitative Determination ◽  
Plasminogen Activator ◽  
Assay System ◽  
Two Stage ◽  
System Activation ◽  
Frozen Plasma

SummaryA modification of the caseinolytic assay for plasminogen is described. This assay system is characterized by the following features :1. Urokinase is used as activator achieving a complete activation of the plasminogen whereas with streptokinase caseinolytically inactive plasminogen-activator complexes are formed.2. All incubation times are reduced to the minimum which is still compatible with accuracy.3. Results are expressed in percent of a standard of ten normal plasmas.4. In this two-stage assay-system (activation of plasminogen to plasmin, digestion of casein by plasmin) both stages proceed simultaneously in the same system, thus the plasmin formed is stabilized “in statu nascendi” by the casein.5. Several conditions (stability of plasminogen in frozen plasma, use of anticoagulants, reproducibility) are defined.

scholarly journals Electrochemical determination of sertraline in pharmaceutical formulation and serum using a gold electrode in a pH 8.4 bicarbonate solution

2021 ◽  
Vol 152 (2) ◽  
pp. 185-192
Author(s):  
Jelena Lović ◽  
Jelena Lađarević ◽  
Nemanja Trišović ◽  
Filip Andrić ◽  
Aleksandar Mladenović ◽  
...  
Keyword(s):  
Gold Electrode ◽  
Electrochemical Determination ◽  
Pharmaceutical Formulation

Electrochemical determination of SrFe12O19 impurity in perovskite ferrites

2021 ◽  
pp. 159677
Author(s):  
S.S. Nikitin ◽  
O.V. Merkulov ◽  
A.D. Bamburov ◽  
M.V. Patrakeev
Keyword(s):  
Electrochemical Determination
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