Handheld device for real-time, quantitative, LAMP-based detection of Salmonella enterica using assimilating probes

2011 ◽  
Vol 30 (1) ◽  
pp. 255-260 ◽  
Author(s):  
Daniel M. Jenkins ◽  
Ryo Kubota ◽  
Jin Dong ◽  
Yong Li ◽  
Dennis Higashiguchi
Keyword(s):  
Real Time ◽  
Salmonella Enterica ◽  
Handheld Device

scholarly journals Real-Time Single Image Depth Perception in the Wild with Handheld Devices

Sensors ◽  
2020 ◽  
Vol 21 (1) ◽  
pp. 15
Author(s):  
Filippo Aleotti ◽  
Giulio Zaccaroni ◽  
Luca Bartolomei ◽  
Matteo Poggi ◽  
Fabio Tosi ◽  
...  
Keyword(s):  
Real Time ◽  
Depth Perception ◽  
Depth Estimation ◽  
Autonomous Driving ◽  
Estimation Methods ◽  
Handheld Devices ◽  
Single Image ◽  
Handheld Device ◽  
Time Performance ◽  
In The Wild

Depth perception is paramount for tackling real-world problems, ranging from autonomous driving to consumer applications. For the latter, depth estimation from a single image would represent the most versatile solution since a standard camera is available on almost any handheld device. Nonetheless, two main issues limit the practical deployment of monocular depth estimation methods on such devices: (i) the low reliability when deployed in the wild and (ii) the resources needed to achieve real-time performance, often not compatible with low-power embedded systems. Therefore, in this paper, we deeply investigate all these issues, showing how they are both addressable by adopting appropriate network design and training strategies. Moreover, we also outline how to map the resulting networks on handheld devices to achieve real-time performance. Our thorough evaluation highlights the ability of such fast networks to generalize well to new environments, a crucial feature required to tackle the extremely varied contexts faced in real applications. Indeed, to further support this evidence, we report experimental results concerning real-time, depth-aware augmented reality and image blurring with smartphones in the wild.

scholarly journals A multiplex real-time PCR assay targeting virulence and resistance genes in Salmonella enterica serotype Typhimurium

2011 ◽  
Vol 11 (1) ◽  
pp. 151 ◽  
Author(s):  
Marie Bugarel ◽  
Sophie A Granier ◽  
François-Xavier Weill ◽  
Patrick Fach ◽  
Anne Brisabois
Keyword(s):  
Real Time ◽  
Resistance Genes ◽  
Real Time Pcr ◽  
Salmonella Enterica ◽  
Pcr Assay

scholarly journals Five Closed Salmonella enterica Genome Sequences from a 2017–2018 Multistrain, Multistate Kratom Outbreak

2020 ◽  
Vol 9 (3) ◽  
Author(s):  
Hallie E. Rauch ◽  
Julie Haendiges ◽  
Maria Balkey ◽  
Maria Hoffmann
Keyword(s):  
Dna Sequencing ◽  
Real Time ◽  
Single Molecule ◽  
Salmonella Enterica ◽  
Genome Sequences ◽  
Circular Chromosome ◽  
Content Type ◽  
Single Plasmid

We report here the closed genomes of Salmonella enterica strains from the 2017–2018 multistrain, multistate kratom outbreak using single-molecule real-time DNA sequencing. Four of the genomes consist of one circular chromosome, and the fifth has a circular chromosome and a single plasmid.

scholarly journals Development of a TaqMan Array Card for Acute-Febrile-Illness Outbreak Investigation and Surveillance of Emerging Pathogens, Including Ebola Virus

2015 ◽  
Vol 54 (1) ◽  
pp. 49-58 ◽  
Author(s):  
Jie Liu ◽  
Caroline Ochieng ◽  
Steve Wiersma ◽  
Ute Ströher ◽  
Jonathan S. Towner ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Salmonella Enterica ◽  
Ebola Virus ◽  
Febrile Illness ◽  
Fever Virus ◽  
Outbreak Investigation ◽  
Acute Febrile Illness ◽  
Content Type ◽  
Taqman Array

Acute febrile illness (AFI) is associated with substantial morbidity and mortality worldwide, yet an etiologic agent is often not identified. Convalescent-phase serology is impractical, blood culture is slow, and many pathogens are fastidious or impossible to cultivate. We developed a real-time PCR-based TaqMan array card (TAC) that can test six to eight samples within 2.5 h from sample to results and can simultaneously detect 26 AFI-associated organisms, including 15 viruses (chikungunya, Crimean-Congo hemorrhagic fever [CCHF] virus, dengue, Ebola virus, Bundibugyo virus, Sudan virus, hantaviruses [Hantaan and Seoul], hepatitis E, Marburg, Nipah virus, o'nyong-nyong virus, Rift Valley fever virus, West Nile virus, and yellow fever virus), 8 bacteria (Bartonellaspp.,Brucellaspp.,Coxiella burnetii,Leptospiraspp.,Rickettsiaspp.,Salmonella entericaandSalmonella entericaserovar Typhi, andYersinia pestis), and 3 protozoa (Leishmaniaspp.,Plasmodiumspp., andTrypanosoma brucei). Two extrinsic controls (phocine herpesvirus 1 and bacteriophage MS2) were included to ensure extraction and amplification efficiency. Analytical validation was performed on spiked specimens for linearity, intra-assay precision, interassay precision, limit of detection, and specificity. The performance of the card on clinical specimens was evaluated with 1,050 blood samples by comparison to the individual real-time PCR assays, and the TAC exhibited an overall 88% (278/315; 95% confidence interval [CI], 84% to 92%) sensitivity and a 99% (5,261/5,326, 98% to 99%) specificity. This TaqMan array card can be used in field settings as a rapid screen for outbreak investigation or for the surveillance of pathogens, including Ebola virus.

scholarly journals Rapid Multiplex PCR and Real-Time TaqMan PCR Assays for Detection of Salmonella enterica and the Highly Virulent Serovars Choleraesuis and Paratyphi C

2008 ◽  
Vol 46 (12) ◽  
pp. 4018-4022 ◽  
Author(s):  
D. F. Woods ◽  
F. J. Reen ◽  
D. Gilroy ◽  
J. Buckley ◽  
J. G. Frye ◽  
...  
Keyword(s):  
Multiplex Pcr ◽  
Real Time ◽  
Salmonella Enterica ◽  
Taqman Pcr ◽  
Pcr Assays ◽  
Highly Virulent
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