A microfluidic device with microbead array for sensitive virus detection and genotyping using quantum dots as fluorescence labels

2010 ◽  
Vol 25 (11) ◽  
pp. 2402-2407 ◽  
Author(s):  
He Zhang ◽  
Tao Xu ◽  
Cheuk-Wing Li ◽  
Mengsu Yang
Keyword(s):  
Quantum Dots ◽  
Microfluidic Device ◽  
Virus Detection ◽  
Microbead Array

Enhanced electrochemiluminescence of CdS quantum dots capped with mercaptopropionic acid activated by EDC for Zika virus detection

The Analyst ◽  
2021 ◽  
Author(s):  
Hui-Jun Zhang ◽  
Jin Zhu ◽  
Ning Bao ◽  
Shou-Nian Ding
Keyword(s):  
Quantum Dots ◽  
Zika Virus ◽  
Virus Detection ◽  
Cds Quantum Dots ◽  
Mercaptopropionic Acid ◽  
Sandwich Type ◽  
Cds Qds

The mechanism of enhanced ECL of MPA@CdS QDs by EDC activation was investigated, and a sandwich-type ECL immunosensor has been designed for Zika virus detection.

Rapid Detection of Norovirus from Fresh Lettuce Using Immunomagnetic Separation and a Quantum Dots Assay

2013 ◽  
Vol 76 (4) ◽  
pp. 707-711 ◽  
Author(s):  
HEE-MIN LEE ◽  
JOSEPH KWON ◽  
JONG-SOON CHOI ◽  
KYEONG-HWAN LEE ◽  
SUNG YANG ◽  
...  
Keyword(s):  
Quantum Dots ◽  
High Speed ◽  
Virus Detection ◽  
Immunomagnetic Separation ◽  
Food Samples ◽  
Pcr Analysis ◽  
Virus Concentration ◽  
Rt Pcr ◽  
Reverse Transcription Pcr ◽  
Centrifugation Step

Current molecular methods that include PCR have been used to detect norovirus in many food samples. However, the protocols require removing PCR inhibitors and incorporate time-consuming concentration steps to separate virus from analyte for rapid and sensitive detection of norovirus. We developed an immunomagnetic separation (IMS) and a quantum dots (QDs) assay to detect norovirus eluted from fresh lettuce with Tris buffer containing 1% beef extract (pH 9.5). IMS facilitated viral precipitation with a 10-min incubation, whereas virus concentration using polyethylene glycol (PEG) requires more than 3 h and an additional high-speed centrifugation step to precipitate virus before reverse transcription PCR (RT-PCR) analysis. The fluorescence intensity of QDs was detected qualitatively on norovirus dilutions of 10−1 to 10−3 in a stool suspension (100 RT-PCR units/ml). The results suggest that a fluorescence assay based on IMS and QDs is valid for detecting norovirus qualitatively according to fluorescent signal intensity within the same virus detection limit produced by IMS–RT-PCR and PEG–RT-PCR.

scholarly journals Fluorometric virus detection platform using quantum dots-gold nanocomposites optimizing the linker length variation

2020 ◽  
Vol 1109 ◽  
pp. 148-157 ◽  
Author(s):  
Fahmida Nasrin ◽  
Ankan Dutta Chowdhury ◽  
Kenshin Takemura ◽  
Ikko Kozaki ◽  
Hiroyuki Honda ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Virus Detection ◽  
Length Variation ◽  
Linker Length ◽  
Gold Nanocomposites

Analysis of Nonadherent Apoptotic Cells by a Quantum Dots Probe in a Microfluidic Device for Drug Screening

2009 ◽  
Vol 81 (16) ◽  
pp. 7075-7080 ◽  
Author(s):  
Liang Zhao ◽  
Peng Cheng ◽  
Jianxin Li ◽  
Yue Zhang ◽  
Miaomiao Gu ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Microfluidic Device ◽  
Drug Screening ◽  
Apoptotic Cells

Controlled Self-Assembly of Quantum Dots and Block Copolymers in a Microfluidic Device

Langmuir ◽  
2008 ◽  
Vol 24 (3) ◽  
pp. 637-643 ◽  
Author(s):  
Greg Schabas ◽  
Huda Yusuf ◽  
Matthew G. Moffitt ◽  
David Sinton
Keyword(s):  
Quantum Dots ◽  
Block Copolymers ◽  
Microfluidic Device ◽  
Self Assembly

Nanostructure of semiconductor quantum dots in a borosilicate glass matrix by complementary use of HREM and AEM

1990 ◽  
Vol 48 (4) ◽  
pp. 728-729
Author(s):  
M.J. Kim ◽  
L.C. Liu ◽  
S.H. Risbud ◽  
R.W. Carpenter
Keyword(s):  
Quantum Dots ◽  
Borosilicate Glass ◽  
Glass Matrix ◽  
Optical Switching ◽  
Response Times ◽  
Fast Response ◽  
Processing Technique ◽  
Internal Stresses ◽  
Electron Hole ◽  
Spatial Dimensions

When the size of a semiconductor is reduced by an appropriate materials processing technique to a dimension less than about twice the radius of an exciton in the bulk crystal, the band like structure of the semiconductor gives way to discrete molecular orbital electronic states. Clusters of semiconductors in a size regime lower than 2R {where R is the exciton Bohr radius; e.g. 3 nm for CdS and 7.3 nm for CdTe) are called Quantum Dots (QD) because they confine optically excited electron- hole pairs (excitons) in all three spatial dimensions. Structures based on QD are of great interest because of fast response times and non-linearity in optical switching applications.In this paper we report the first HREM analysis of the size and structure of CdTe and CdS QD formed by precipitation from a modified borosilicate glass matrix. The glass melts were quenched by pouring on brass plates, and then annealed to relieve internal stresses. QD precipitate particles were formed during subsequent "striking" heat treatments above the glass crystallization temperature, which was determined by differential thermal analysis.

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