Sensitive enzyme-biosensor based on screen-printed electrodes for Ochratoxin A

2010 ◽  
Vol 25 (6) ◽  
pp. 1333-1337 ◽  
Author(s):  
M. Asunción Alonso-Lomillo ◽  
Olga Domínguez-Renedo ◽  
Liliana Ferreira-Gonçalves ◽  
M. Julia Arcos-Martínez
Toxins ◽  
2018 ◽  
Vol 10 (8) ◽  
pp. 317 ◽  
Author(s):  
Xian Zhang ◽  
Zuohuan Wang ◽  
Hui Xie ◽  
Renjie Sun ◽  
Tong Cao ◽  
...  

A rapid and sensitive electrochemical biosensor based on magnetic nanoparticles and screen-printed electrodes (MNPs-SPEs sensor) was developed for the detection of ochratoxin A (OTA) in cereal and feed samples. Different types of magnetic nanoparticles-based ELISA (MNPs-ELISA) were optimized, and the signal detection, as well as sensitivity, was enhanced by the combined use of screen-printed electrodes (SPEs). Under the optimized conditions, the calibration curve of the MNPs-SPEs sensor was y = 0.3372x + 0.8324 (R2 = 0.9805). The linear range of detection and the detection limit were 0.01–0.82 ng/mL and 0.007 ng/mL, respectively. In addition, 50% inhibition (IC50) was detectable at 0.10 ng/mL. The limit of detection (LOD) of this MNPs-SPEs sensor in cereal and feed samples was 0.28 μg/kg. The recovery rates in spiked samples were between 78.7% and 113.5%, and the relative standard deviations (RSDs) were 3.6–9.8%, with the coefficient of variation lower than 15%. Parallel analysis of commercial samples (corn, wheat, and feedstuff) showed a good correlation between MNPs-SPEs sensor and liquid chromatography tandem mass spectrometry (LC/MS-MS). This new method provides a rapid, highly sensitive, and less time-consuming method to determine levels of ochratoxin A in cereal and feedstuff samples.


Biosensors ◽  
2016 ◽  
Vol 6 (3) ◽  
pp. 33 ◽  
Author(s):  
Francesca Malvano ◽  
Donatella Albanese ◽  
Alessio Crescitelli ◽  
Roberto Pilloton ◽  
Emanuela Esposito

Biosensors ◽  
2017 ◽  
Vol 7 (4) ◽  
pp. 50 ◽  
Author(s):  
Gonzalo Martínez-García ◽  
Elena Pérez-Julián ◽  
Lourdes Agüí ◽  
Naomí Cabré ◽  
Jorge Joven ◽  
...  

2011 ◽  
Vol 56 (11) ◽  
pp. 3988-3995 ◽  
Author(s):  
Elena Jubete ◽  
Kamila Żelechowska ◽  
Oscar A. Loaiza ◽  
Pedro J. Lamas ◽  
Estibalitz Ochoteco ◽  
...  

Author(s):  
Robert D. Crapnell ◽  
Whitchuta Jesadabundit ◽  
Alejandro García-Miranda Ferrari ◽  
Nina C. Dempsey-Hibbert ◽  
Marloes Peeters ◽  
...  

2021 ◽  
pp. 101055
Author(s):  
Dora Domnica Baciu ◽  
Ruxandra Bîrjega ◽  
Valentina Mărăscu ◽  
Rodica Zăvoianu ◽  
Andreea Matei ◽  
...  

2003 ◽  
Vol 481 (2) ◽  
pp. 209-211 ◽  
Author(s):  
Christine Bonnet ◽  
Silvana Andreescu ◽  
Jean-Louis Marty

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