A fully electronic sensor for the measurement of cDNA hybridization kinetics

2007 ◽  
Vol 22 (9-10) ◽  
pp. 2108-2114 ◽  
Author(s):  
L. Bandiera ◽  
G. Cellere ◽  
S. Cagnin ◽  
A. De Toni ◽  
E. Zanoni ◽  
...  
Keyword(s):  
Electronic Sensor ◽  
Cdna Hybridization

scholarly journals Accuracy of soft tissue balancing in total knee arthroplasty using surgeon-defined assessment versus a gap-balancer or electronic sensor

2021 ◽  
Vol 16 (1) ◽  
Author(s):  
Ran Zhao ◽  
Yanqing Liu ◽  
Hua Tian
Keyword(s):  
Total Knee Arthroplasty ◽  
Soft Tissue ◽  
Knee Arthroplasty ◽  
Sensor Data ◽  
Soft Tissue Balancing ◽  
Electronic Sensor ◽  
Prior Performance ◽  
Cumulative Summation ◽  
Total Knee ◽  
Sensitivity Specificity

Abstract Background Soft tissue balancing is essential for the success of total knee arthroplasty (TKA) and is mainly dependent on surgeon-defined assessment (SDA) or a gap-balancer (GB). However, an electronic sensor has been developed to objectively measure the gap pressure. This study aimed to evaluate the accuracy of soft tissue balancing using SDA and GB compared with a sensor. Methods Forty-eight patients undergoing TKA (60 knees) were prospectively enrolled. Soft tissue balancing was sequentially performed using SDA, a GB, and an electronic sensor. We compared the SDA, GB, and sensor data to calculate the sensitivity, specificity, and accuracy at 0°, 45°, 90°, and 120° flexion. Cumulative summation (CUSUM) analysis was performed to assess the surgeon’s performance during the sensor introductory phase. Results The sensitivity of SDA was 63.3%, 68.3%, 80.0%, and 80.0% at 0°, 45°, 90°, and 120°, respectively. The accuracy of the GB compared with sensor data was 76.7% and 71.7% at 0° and 90°, respectively. Cohen’s kappa coefficient for the accuracy of the GB was 0.406 at 0° (moderate agreement) and 0.227 at 90° (fair agreement). The CUSUM 0° line achieved good prior performance at case 45, CUSUM 90° and 120° showed a trend toward good prior performance, while CUSUM 45° reached poor prior performance at case 8. Conclusion SDA was a poor predictor of knee balance. GB improved the accuracy of soft tissue balancing, but was still less accurate than the sensor, particularly for unbalanced knees. SDA improved with ongoing use of the sensor, except at 45° flexion.

Electronic sensor system for registering ECG and PPG signals

2021 ◽  
Author(s):  
Krasimir Cheshmedzhiev ◽  
Evgeniya Gospodinova ◽  
Mitko Gospodinov ◽  
Penio Lebamovski
Keyword(s):  
Sensor System ◽  
Electronic Sensor

Electronic Sensor Design Principles

2021 ◽  
Author(s):  
Marco Tartagni
Keyword(s):  
Graduate Students ◽  
Design Process ◽  
Interdisciplinary Approach ◽  
Process Efficiency ◽  
Sensor Design ◽  
Overall Design ◽  
Electronic Sensor ◽  
Speed Up ◽  
Design Requirements ◽  
Electronic Sensors

Get up to speed with the fundamentals of electronic sensor design with this comprehensive guide, and discover powerful techniques to reduce the overall design timeline for your specific applications. Includes a step-by-step introduction to a generalized information-centric approach for designing electronic sensors, demonstrating universally applicable practical approaches to speed up the design process. Features detailed coverage of all the tools necessary for effective characterization and organization of the design process, improving overall process efficiency. Provides a coherent and rigorous theoretical framework for understanding the fundamentals of sensor design, to encourage an intuitive understanding of sensor design requirements. Emphasising an integrated interdisciplinary approach throughout, this is an essential tool for professional engineers and graduate students keen to improve their understanding of cutting-edge electronic sensor design.

Matrigel induces thymosin beta 4 gene in differentiating endothelial cells

1995 ◽  
Vol 108 (12) ◽  
pp. 3685-3694 ◽  
Author(s):  
D.S. Grant ◽  
J.L. Kinsella ◽  
M.C. Kibbey ◽  
S. LaFlamme ◽  
P.D. Burbelo ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Basement Membrane ◽  
Morphological Differentiation ◽  
Tube Formation ◽  
Vessel Formation ◽  
Thymosin Beta 4 ◽  
Cdna Hybridization ◽  
Matrix Components ◽  
Cells Cultured

We performed differential cDNA hybridization using RNA from endothelial cells cultured for 4 hours on either plastic or basement membrane matrix (Matrigel), and identified early genes induced during the morphological differentiation into capillary-like tubes. The mRNA for one clone, thymosin beta 4, was increased 5-fold. Immunostaining localized thymosin beta 4 in vivo in both growing and mature vessels as well as in other tissues. Endothelial cells transfected with thymosin beta 4 showed an increased rate of attachment and spreading on matrix components, and an accelerated rate of tube formation on Matrigel. An antisense oligo to thymosin beta 4 inhibited tube formation on Matrigel. The results suggest that thymosin beta 4 is induced and likely involved in differentiating endothelial cells. Thymosin beta 4 may play a role in vessel formation in vivo.

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