Design and development of a highly stable hydrogen peroxide biosensor on screen printed carbon electrode based on horseradish peroxidase bound with gold nanoparticles in the matrix of chitosan

2007 ◽  
Vol 22 (9-10) ◽  
pp. 2071-2078 ◽  
Author(s):  
Tanin Tangkuaram ◽  
Chatchai Ponchio ◽  
Thippayawadee Kangkasomboon ◽  
Panadda Katikawong ◽  
Waret Veerasai
Keyword(s):  
Hydrogen Peroxide ◽  
Gold Nanoparticles ◽  
Horseradish Peroxidase ◽  
Carbon Electrode ◽  
Design And Development ◽  
Hydrogen Peroxide Biosensor ◽  
Screen Printed Carbon Electrode ◽  
The Matrix ◽  
Screen Printed

scholarly journals Development of Hydrogen Peroxide Biosensor Based on Immobilization of Hemoglobin on Screen-Printed Carbon Electrode Modified With Silver Nanoparticles

2019 ◽  
pp. 2332-2340
Author(s):  
Ali Saad Elewi ◽  
Shatha Abdul Wadood ◽  
Abdul Kareem Mohammed Ali
Keyword(s):  
Hydrogen Peroxide ◽  
Silver Nanoparticles ◽  
Electron Transfer ◽  
Direct Electron Transfer ◽  
Phosphate Buffer Solution ◽  
Carbon Electrode ◽  
Buffer Solution ◽  
Hydrogen Peroxide Biosensor ◽  
Screen Printed Carbon Electrode ◽  
Screen Printed

     The direct electron transfer behavior of hemoglobin that is immobilized onto screen-printed carbon electrode (SPCE) modified with silver nanoparticles (AgNPs) and chitosan (CS) was studied in this work. Cyclic voltametry and spectrophotometry were used to characterize the hemoglobin (Hb) bioconjunction with AgNPs and CS. Results of the modified electrode showed quasi-reversible redox peaks with a formal potential of (-0.245V) versus Ag/AgCl in 0.1M phosphate buffer solution (PBS), pH7, at a scan rate of 0.1Vs-1. The charge transfer coefficient (α) was 0.48 and the apparent electron transfer rate constant (Ks) was 0.47s-1. The electrode was used as a hydrogen peroxide biosensor with a linear response over 3 to 240 µM and a detection limit of 0.6 µM. As a result, the modified biosensor here has exhibited a high sensitivity, good reproducibility and stability.

Biological Multilayer Films Assembled by Redox Polymer and HRP on Screen-Printed Carbon Electrode

2012 ◽  
Vol 571 ◽  
pp. 56-59
Author(s):  
Yu Fang Sha ◽  
Mei Zhao ◽  
Ming Quan Yang ◽  
Hai Xin Bai ◽  
Man Zhao
Keyword(s):  
Horseradish Peroxidase ◽  
Electrostatic Interaction ◽  
Electrode Surface ◽  
Multilayer Films ◽  
Carbon Electrode ◽  
Layer By Layer ◽  
Redox Polymer ◽  
Screen Printed Carbon Electrode ◽  
Positively Charged ◽  
Screen Printed

Biological multilayer films of redox polymer and horseradish peroxidase (HRP) were successfully assembled on a screen-printed carbon electrode using layer-by-layer (LBL) assembled method based on the electrostatic interaction. The screen-printed carbon electrode surface was modified by the positively charged redox polymer, and the negatively charged HRP by LBL method.

A microfluidic device based on a screen-printed carbon electrode with electrodeposited gold nanoparticles for the detection of IgG anti-Trypanosoma cruzi antibodies

The Analyst ◽  
2011 ◽  
Vol 136 (22) ◽  
pp. 4745 ◽  
Author(s):  
Sirley V. Pereira ◽  
Franco A. Bertolino ◽  
Martín A. Fernández-Baldo ◽  
Germán A. Messina ◽  
Eloy Salinas ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Trypanosoma Cruzi ◽  
Microfluidic Device ◽  
Carbon Electrode ◽  
Screen Printed Carbon Electrode ◽  
Electrodeposited Gold Nanoparticles ◽  
Screen Printed
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