Label-free reading of microarray-based proteins with high throughput surface plasmon resonance imaging

2006 ◽  
Vol 22 (5) ◽  
pp. 644-648 ◽  
Author(s):  
Haowen Huang ◽  
Yi Chen
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
High Throughput ◽  
Plasmon Resonance ◽  
Surface Plasmon Resonance Imaging ◽  
Label Free ◽  
Resonance Imaging

scholarly journals Rapid and Label-Free Immunosensing of Shiga Toxin Subtypes with Surface Plasmon Resonance Imaging

Toxins ◽  
2020 ◽  
Vol 12 (5) ◽  
pp. 280
Author(s):  
Bin Wang ◽  
Bosoon Park ◽  
Jing Chen ◽  
Xiaohua He
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
High Throughput ◽  
Plasmon Resonance ◽  
Shiga Toxin ◽  
The United States ◽  
Surface Plasmon Resonance Imaging ◽  
Label Free ◽  
Resonance Imaging ◽  
Shiga Toxins

Shiga toxin-producing Escherichia coli (STEC) are responsible for gastrointestinal diseases reported in numerous outbreaks around the world as well as in the United States. Current detection methods have limitation to implement for rapid field-deployable detection with high volume of samples that are needed for regulatory purposes. Surface plasmon resonance imaging (SPRi) has proved to achieve rapid and label-free screening of multiple pathogens simultaneously, so it was evaluated in this work for the detection of Shiga toxins (Stx1a and Stx2a toxoids were used as the less toxic alternatives to Stx1 and Stx2, respectively). Multiple antibodies (Stx1pAb, Stx1-1mAb, Stx1-2mAb, Stx1d-3mAb, Stx1e-4mAb, Stx2pAb, Stx2-1mAb, Stx2-2mAb, and Stx2-10mAb) were spotted one by one by programed microarrayer, on the same high-throughput biochip with 50-nm gold film through multiple crosslinking and blocking steps to improve the orientation of antibodies on the biochip surface. Shiga toxins were detected based on the SPRi signal difference (ΔR) between immobilized testing antibodies and immunoglobulin G (IgG) control. Among the antibodies tested, Stx1pAb showed the highest sensitivity for Stx1 toxoid, with the limit of detection (LOD) of 50 ng/mL and detection time of 20 min. Both Stx2-1mAb and Stx2-2mAb exhibited high sensitivity for Stx2 toxoid. Furthermore, gold nanoparticles (GNPs) were used to amplify the SPRi signals of monoclonal antibodies in a sandwich platform. The LOD reached the level of picogram (pg)/mL with the help of GNP-antibody conjugate. This result proved that SPRi biochip with selected antibodies has the potential for rapid, high-throughput and multiplex detection of Shiga toxins.

Electrokinetic label-free screening chip: a marriage of multiplexing and high throughput analysis using surface plasmon resonance imaging

Lab on a Chip ◽  
2010 ◽  
Vol 10 (8) ◽  
pp. 986 ◽  
Author(s):  
Ganeshram Krishnamoorthy ◽  
Edwin T. Carlen ◽  
Johan G. Bomer ◽  
Daniël Wijnperlé ◽  
Hans L. deBoer ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
High Throughput ◽  
Plasmon Resonance ◽  
Surface Plasmon Resonance Imaging ◽  
Label Free ◽  
Resonance Imaging ◽  
High Throughput Analysis ◽  
Throughput Analysis

LABEL-FREE DETECTION OF PROTEIN MICROARRAY WITH HIGH THROUGHPUT SURFACE PLASMON RESONANCE IMAGING (SPRI)

2008 ◽  
Vol 01 (01) ◽  
pp. 107-114 ◽  
Author(s):  
SHUKUAN XU ◽  
GUOLIANG HUANG ◽  
CHENG DENG ◽  
JIANG ZHU ◽  
CHAO HAN ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
High Throughput ◽  
Plasmon Resonance ◽  
Incident Angle ◽  
Protein Microarray ◽  
Surface Plasmon Resonance Imaging ◽  
Label Free ◽  
Resonance Imaging ◽  
Label Free Detection

A surface plasmon resonance imaging (SPRI) system was developed for the discrimination of proteins on a gold surface. As a label-free and high-throughput technique, SPRI enables simultaneously monitoring of the biomolecular interactions at low concentrations. We used SPRI as a label-free and parallel method to detect different proteins based on protein microarray. Bovine Serum Albumin (BSA), Casein and Immunoglobulin G (IgG) were immobilized onto the Au surface of a gold-coated glass chip as spots forming a 6 × 6 matrix. These proteins can be discriminated directly by changing the incident angle of light. Excellent reproducibility for label-free detection of protein molecules was achieved. This SPRI platform represents a simple and robust method for performing high-sensitivity detection of protein microarray.

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