A nanoparticle amplification based quartz crystal microbalance DNA sensor for detection of Escherichia coli O157:H7

2006 ◽  
Vol 21 (7) ◽  
pp. 1178-1185 ◽  
Author(s):  
Xiaole Mao ◽  
Liju Yang ◽  
Xiao-Li Su ◽  
Yanbin Li
Author(s):  
Vo Ke Thanh Ngo ◽  
Dang Giang Nguyen ◽  
Hoang Phuong Uyen Nguyen ◽  
Van Man Tran ◽  
Thi Khoa My Nguyen ◽  
...  

The Analyst ◽  
2020 ◽  
Vol 145 (6) ◽  
pp. 2267-2278 ◽  
Author(s):  
Rehan Deshmukh ◽  
Arun Kumar Prusty ◽  
Utpal Roy ◽  
Sunil Bhand

We report a label-free biosensor for the detection of Escherichia coli O157:H7 ATCC 43895 in potable water using a newly designed DNA sensing probe targeting the z3276 genetic marker.


Langmuir ◽  
2011 ◽  
Vol 27 (8) ◽  
pp. 4900-4905 ◽  
Author(s):  
Jia Xu ◽  
Kai-Wei Liu ◽  
Kathleen S. Matthews ◽  
Sibani L. Biswal

2008 ◽  
Vol 8 (9) ◽  
pp. 4553-4556 ◽  
Author(s):  
Dong-Yun Lee ◽  
Won-Suk Choi ◽  
A. K. M. Kafi ◽  
Sang-Hyun Park ◽  
Young-Soo Kwon

A synthesized 21-mer single-stranded DNA (ssDNA) was covalently immobilized onto a self-assembled aminoethanethiol monolayer modified gold electrode onto quartz crystal microbalance (QCM). The covalently immobilized ssDNA was hybridized with complementary ssDNA. The interaction between surface-immobilized ssDNA and complementary 21-mer DNA in solution was also examined. Each step was followed by monitoring changes in the QCM frequency over time. From the results, the measured frequency change was about 256 Hz, 160 Hz and 141 Hz, respectively. As a result, the adsorption mass was about 273 ng/cm2, 171 ng/cm2 and 151 ng/cm2, according to the Sauerbrey equation. Also, PBS with pH 7.0 was selected as a supporting electrolyte to achieve maximum sensitivity and good bioactivity. The anodic peak current was linearly related to the concentrations between 1 × 10−5 and 9 × 10−5 M with a detection limit of 1.0 × 10 −6 M.


2014 ◽  
Vol 12 (6) ◽  
pp. 1152-1155 ◽  
Author(s):  
Renata Samardzic ◽  
Hermann F. Sussitz ◽  
Nathjanan Jongkon ◽  
Peter A. Lieberzeit

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