Carbon-nanotube-modified electrodes for amplified enzyme-based electrical detection of DNA hybridization

2004 ◽  
Vol 20 (5) ◽  
pp. 995-1000 ◽  
Author(s):  
J WANG
Keyword(s):  
Carbon Nanotube ◽  
Dna Hybridization ◽  
Modified Electrodes ◽  
Electrical Detection

Label-Free Electrochemical Detection of DNA Hybridization Related to Anthrax Lethal Factor by using Carbon Nanotube Modified Sensors

2019 ◽  
Vol 15 (4) ◽  
pp. 502-510 ◽  
Author(s):  
Hakan Karadeniz ◽  
Arzum Erdem
Keyword(s):  
Detection Limit ◽  
Dna Hybridization ◽  
Lethal Factor ◽  
Modified Electrodes ◽  
Label Free ◽  
Carbon Paste ◽  
Chip Technology ◽  
Anthrax Lethal Factor ◽  
Hybridization Time ◽  
Electrochemical Monitoring

Background: Anthrax Lethal Factor (ANT) is the dominant virulence factor produced by B. anthracis and is the major cause of death of infected animals. In this paper, pencil graphite electrodes GE were modified with single-walled and multi-walled carbon nanotubes (CNTs) for the detection of hybridization related to the ANT DNA for the first time in the literature. Methods: The electrochemical monitoring of label-free DNA hybridization related to ANT DNA was explored using both SCNT and MCNT modified PGEs with differential pulse voltammetry (DPV). The performance characteristics of ANT-DNA hybridization on disposable GEs were explored by measuring the guanine signal in terms of optimum analytical conditions; the concentration of SCNT and MCNT, the concentrations of probe and target, and also the hybridization time. Under the optimum conditions, the selectivity of probe modified electrodes was tested and the detection limit was calculated. Results: The selectivity of ANT probes immobilized onto MCNT-GEs was tested in the presence of hybridization of probe with NC no response was observed and with MM, smaller responses were observed in comparison to full-match DNA hybridization case. Even though there are unwanted substituents in the mixture samples containing both the target and NC in the ratio 1:1 and both the target and MM in the ratio 1:1, it has been found that ANT probe immobilized CNT modified graphite sensor can also select its target by resulting with 20.9% decreased response in comparison to the one measured in the case of full-match DNA hybridization case Therefore, it was concluded that the detection of direct DNA hybridization was performed by using MCNT-GEs with an acceptable selectivity. Conclusion: Disposable SCNT/MCNT modified GEs bring some important advantages to our assay including easy use, cost-effectiveness and giving a response in a shorter time compared to unmodified PGE, carbon paste electrode and glassy carbon electrode developed for electrochemical monitoring of DNA hybridization. Consequently, the detection of DNA hybridization related to the ANT DNA by MCNT modified sensors was performed by using lower CNT, probe and target concentrations, in a shorter hybridization time and resulting in a lower detection limit according to the SCNT modified sensors. In conclusion, MCNT modified sensors can yield the possibilities leading to the development of nucleic acid sensors platforms for the improvement of fast and cost-effective detection systems with respect to DNA chip technology.

Electrocatalysis at graphite and carbon nanotube modified electrodes: edge-plane sites and tube ends are the reactive sites

2005 ◽  
pp. 829 ◽  
Author(s):  
Craig E. Banks ◽  
Trevor J. Davies ◽  
Gregory G. Wildgoose ◽  
Richard G. Compton
Keyword(s):  
Carbon Nanotube ◽  
Modified Electrodes

Label-Free Electrical Detection of DNA Hybridization on Graphene using Hall Effect Measurements: Revisiting the Sensing Mechanism

2013 ◽  
Vol 23 (18) ◽  
pp. 2301-2307 ◽  
Author(s):  
Cheng-Te Lin ◽  
Phan Thi Kim Loan ◽  
Tzu-Yin Chen ◽  
Keng-Ku Liu ◽  
Chang-Hsiao Chen ◽  
...  
Keyword(s):  
Hall Effect ◽  
Dna Hybridization ◽  
Label Free ◽  
Electrical Detection ◽  
Sensing Mechanism ◽  
Hall Effect Measurements

Electrochemical detection of phenolic estrogenic compounds at carbon nanotube-modified electrodes

Talanta ◽  
2007 ◽  
Vol 71 (3) ◽  
pp. 1031-1038 ◽  
Author(s):  
D. Vega ◽  
L. Agüí ◽  
A. González-Cortés ◽  
P. Yáñez-Sedeño ◽  
J.M. Pingarrón
Keyword(s):  
Carbon Nanotube ◽  
Electrochemical Detection ◽  
Modified Electrodes ◽  
Estrogenic Compounds

Electrical detection of nitric oxide using single-walled carbon nanotube network devices

Carbon ◽  
2007 ◽  
Vol 45 (9) ◽  
pp. 1911-1914 ◽  
Author(s):  
Dongliang Fu ◽  
Yanping Xu ◽  
Lain-Jong Li ◽  
Y. Chen ◽  
S.G. Mhaisalkar ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Carbon Nanotube ◽  
Electrical Detection ◽  
Single Walled Carbon Nanotube ◽  
Single Walled Carbon ◽  
Carbon Nanotube Network

Development of a microbial biosensor based on carbon nanotube (CNT) modified electrodes

2007 ◽  
Vol 9 (7) ◽  
pp. 1810-1815 ◽  
Author(s):  
Suna Timur ◽  
Ulku Anik ◽  
Dilek Odaci ◽  
Lo Gorton
Keyword(s):  
Carbon Nanotube ◽  
Modified Electrodes ◽  
Microbial Biosensor
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