Identification and characterization of Bacillus anthracis by multiplex PCR on DNA chip

2004 ◽  
Vol 20 (4) ◽  
pp. 807-813 ◽  
Author(s):  
Shi-Hua Wang ◽  
Ji-Kai Wen ◽  
Ya-Feng Zhou ◽  
Zhi-Ping Zhang ◽  
Rui-Fu Yang ◽  
...  
Keyword(s):  
Bacillus Anthracis ◽  
Multiplex Pcr ◽  
Dna Chip ◽  
Identification And Characterization

scholarly journals Molecular characterization of Bacillus anthracis using multiplex PCR, ERIC-PCR and RAPD

2001 ◽  
Vol 32 (3) ◽  
pp. 139-145 ◽  
Author(s):  
Y.-H. Shangkuan ◽  
Y.-H. Chang ◽  
J.-F. Yang ◽  
H.-C. Lin ◽  
M.-F. Shaio
Keyword(s):  
Bacillus Anthracis ◽  
Multiplex Pcr ◽  
Molecular Characterization

scholarly journals Identification and characterization of clinical Bacillus spp. isolates phenotypically similar to Bacillus anthracis

2010 ◽  
Vol 313 (1) ◽  
pp. 47-53 ◽  
Author(s):  
Cari A. Beesley ◽  
Cynthia L. Vanner ◽  
Leta O. Helsel ◽  
Jay E. Gee ◽  
Alex R. Hoffmaster
Keyword(s):  
Bacillus Anthracis ◽  
Bacillus Spp ◽  
Identification And Characterization

scholarly journals Identification and Characterization of the gerH Operon of Bacillus anthracis Endospores: a Differential Role for Purine Nucleosides in Germination

2003 ◽  
Vol 185 (4) ◽  
pp. 1462-1464 ◽  
Author(s):  
Matthew A. Weiner ◽  
Timothy D. Read ◽  
Philip C. Hanna
Keyword(s):  
Amino Acids ◽  
Bacillus Anthracis ◽  
Germination Response ◽  
Identification And Characterization

ABSTRACT We identified a tri-cistronic operon, gerH, in Bacillus anthracis that is important for endospore germination triggered by two distinct germination response pathways termed inosine-His and purine-Ala. Together, the two pathways allow B. anthracis endospores a broader recognition of purines and amino acids that may be important for host-mediated germination.

Identification and characterization of a germination operon on the virulence plasmid pXOl of Bacillus anthracis

1999 ◽  
Vol 33 (2) ◽  
pp. 407-414 ◽  
Author(s):  
Chantal Guidi-Rontani ◽  
Yannick Pereira ◽  
Stephanie Ruffie ◽  
Jean-Claude Sirard ◽  
Martine Weber-Levy ◽  
...  
Keyword(s):  
Bacillus Anthracis ◽  
Virulence Plasmid ◽  
Identification And Characterization

Development of a Multiplex PCR for Rapid Detection of Verocytotoxin-Producing Escherichia coli O26 in Raw Milk and Ground Beef

2011 ◽  
Vol 74 (1) ◽  
pp. 13-17 ◽  
Author(s):  
V. LORUSSO ◽  
A. DAMBROSIO ◽  
N. C. QUAGLIA ◽  
A. PARISI ◽  
G. LASALANDRA ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Multiplex Pcr ◽  
Rapid Detection ◽  
Ground Beef ◽  
Raw Milk ◽  
The United States ◽  
Pcr Assay ◽  
E Coli ◽  
Identification And Characterization

Verocytotoxin-producing Escherichia coli (VTEC) O26 is an emergent pathotype that has caused an increasing number of sporadic cases and outbreaks of gastroenteritis, hemorrhagic colitis, and hemolytic uremic syndrome in the United States and Europe. Many cases are associated with the consumption of milk and undercooked or fermented meats. The stx2 strains of VTEC O26 seem to be more likely to cause human infections than isolates expressing only stx1. The isolation and identification of VTEC O26 from foods is labor intensive and time-consuming. We developed a multiplex PCR (M-PCR) assay for the identification and characterization of E. coli O26 VTEC and its detection in raw milk and ground beef. The method is based on the amplification of the wzx, stx1, and stx2 genes for the simultaneous detection of the O26 antigen and verocytotoxin types 1 and 2. This M-PCR assay had a sensitivity of 108 CFU/ml when applied to a bacterial suspension and of 106 CFU/ml or g when applied to both inoculated milk and minced beef samples. This M-PCR assay also was highly specific, and results were consistently negative for negative controls (nonpathogenic E. coli strains, uninoculated milk and beef samples, and samples inoculated with the nontarget microorganisms). This method could be used for the rapid detection of E. coli O26 VTEC from foods and for the rapid identification and characterization of clinical and environmental isolates.

Identification and characterization of immunodominant B-cell epitope of the C-terminus of protective antigen of Bacillus anthracis

2009 ◽  
Vol 46 (10) ◽  
pp. 2107-2115 ◽  
Author(s):  
Manpreet Kaur ◽  
Hema Chug ◽  
Harpreet Singh ◽  
Subhash Chandra ◽  
Manish Mishra ◽  
...  
Keyword(s):  
B Cell ◽  
Bacillus Anthracis ◽  
Protective Antigen ◽  
Cell Epitope ◽  
C Terminus ◽  
B Cell Epitope ◽  
Identification And Characterization
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