Design and optimization of bioreactor to boost carbon dioxide assimilation in RuBisCo-equipped Escherichia coli

2020 ◽  
Vol 314 ◽  
pp. 123785 ◽  
Author(s):  
Shih-I Tan ◽  
I-Son Ng
Keyword(s):  
Escherichia Coli ◽  
Carbon Dioxide ◽  
Carbon Dioxide Assimilation ◽  
Design And Optimization

scholarly journals HEAVY CARBON AS A TRACER IN HETEROTROPHIC CARBON DIOXIDE ASSIMILATION

1941 ◽  
Vol 139 (1) ◽  
pp. 365-376 ◽  
Author(s):  
H.G. Wood ◽  
C.H. Werkman ◽  
Allan Hemingway ◽  
A.O. Nier
Keyword(s):  
Carbon Dioxide ◽  
Carbon Dioxide Assimilation ◽  
Heavy Carbon

An overview of flow cell architectures design and optimization for electrochemical CO2 reduction

2021 ◽  
Author(s):  
Dui Ma ◽  
Ting Jin ◽  
Keyu Xie ◽  
Haitao Huang
Keyword(s):  
Carbon Dioxide ◽  
Global Warming ◽  
Electrochemical Reduction ◽  
Atmospheric Carbon Dioxide ◽  
Co2 Reduction ◽  
Value Added ◽  
Flow Cell ◽  
Design And Optimization ◽  
Electrochemical Co2 Reduction ◽  
Carbon Dioxide Levels

Converting CO2 into value-added fuels or chemical feedstocks through electrochemical reduction is one of the several promising avenues to reduce atmospheric carbon dioxide levels and alleviate global warming. This approach...

Nitrogen Economy and Canopy Carbon Dioxide Assimilation of Tropical Lowland Rice

1990 ◽  
Vol 82 (3) ◽  
pp. 451-459 ◽  
Author(s):  
H. F. Schnier ◽  
M. Dingkuhn ◽  
S. K. De Datta ◽  
K. Mengel ◽  
E. Wijangco ◽  
...  
Keyword(s):  
Carbon Dioxide ◽  
Lowland Rice ◽  
Carbon Dioxide Assimilation ◽  
Nitrogen Economy ◽  
Tropical Lowland

scholarly journals Effect of high-pressure carbon dioxide processing on the inactivation of aerobic mesophilic bacteria and Escherichia coli in human milk

2017 ◽  
Vol 16 (1) ◽  
pp. 122-126 ◽  
Author(s):  
Ana Claudia Berenhauser ◽  
Douglas Soares ◽  
Norton Komora ◽  
Juliano De Dea Lindner ◽  
Elane Schwinden Prudêncio ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Carbon Dioxide ◽  
High Pressure ◽  
Human Milk ◽  
Mesophilic Bacteria

scholarly journals Harnessing Escherichia coli for bio-based production of formate under pressurized H 2 and CO 2 gases.

2021 ◽  
Author(s):  
Magali Roger ◽  
Thomas C. P. Reed ◽  
Frank Sargent
Keyword(s):  
Escherichia Coli ◽  
Carbon Dioxide ◽  
Formic Acid ◽  
Carbon Capture ◽  
Continuous Production ◽  
Carbon Capture And Storage ◽  
Physiological Role ◽  
Anaerobic Growth ◽  
Formate Hydrogenlyase ◽  
And Storage

Escherichia coli is gram-negative bacterium that is a workhorse for biotechnology. The organism naturally performs a mixed-acid fermentation under anaerobic conditions where it synthesises formate hydrogenlyase (FHL-1). The physiological role of the enzyme is the disproportionation of formate in to H 2 and CO 2 . However, the enzyme has been observed to catalyse hydrogenation of CO 2 given the correct conditions, and so has possibilities in bio-based carbon capture and storage if it can be harnessed as a hydrogen-dependent CO 2 -reductase (HDCR). In this study, an E. coli host strain was engineered for the continuous production of formic acid from H 2 and CO 2 during bacterial growth in a pressurised batch bioreactor. Incorporation of tungsten, in place of molybdenum, in FHL-1 helped to impose a degree of catalytic bias on the enzyme. This work demonstrates that it is possible to couple cell growth to simultaneous, unidirectional formate production from carbon dioxide and develops a process for growth under pressurised gases. IMPORTANCE Greenhouse gas emissions, including waste carbon dioxide, are contributing to global climate change. A basket of solutions is needed to steadily reduce emissions, and one approach is bio-based carbon capture and storage. Here we present out latest work on harnessing a novel biological solution for carbon capture. The Escherichia coli formate hydrogenlyase (FHL-1) was engineered to be constitutively expressed. Anaerobic growth under pressurised H 2 and CO 2 gases was established and aqueous formic acid was produced as a result. Incorporation of tungsten in to the enzyme in place of molybdenum proved useful in poising FHL-1 as a hydrogen-dependent CO 2 reductase (HDCR).

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