Cellulase interacts with Lactobacillus plantarum to affect chemical composition, bacterial communities, and aerobic stability in mixed silage of high-moisture amaranth and rice straw

2020 ◽  
Vol 315 ◽  
pp. 123772
Author(s):  
Lin Mu ◽  
Zhan Xie ◽  
Longxing Hu ◽  
Guihua Chen ◽  
Zhifei Zhang
Keyword(s):  
Chemical Composition ◽  
Lactobacillus Plantarum ◽  
Rice Straw ◽  
Bacterial Communities ◽  
High Moisture ◽  
Aerobic Stability

scholarly journals Effects of natamycin and Lactobacillus plantarum on the chemical composition, microbial community, and aerobic stability of Hybrid pennisetum at different temperatures

RSC Advances ◽  
2020 ◽  
Vol 10 (15) ◽  
pp. 8692-8702 ◽  
Author(s):  
Assar Ali Shah ◽  
Chen Qian ◽  
Juanzi Wu ◽  
Zhiwei Liu ◽  
Salman Khan ◽  
...  
Keyword(s):  
Microbial Community ◽  
Chemical Composition ◽  
Lactobacillus Plantarum ◽  
Aerobic Stability ◽  
Different Temperatures ◽  
Fermentation Quality ◽  
Hybrid Pennisetum

The inoculation of L. plantarum and natamycin influenced the fermentation quality. Natamycin and L. plantarum reduced the undesirable microbial community. During ensiling process, the LA and LABs was significantly enhanced.

scholarly journals Changes in the nutritive value and aerobic stability of corn silages inoculated with Bacillus subtilis alone or combined with Lactobacillus plantarum

2016 ◽  
Vol 56 (11) ◽  
pp. 1867 ◽  
Author(s):  
Erika C. Lara ◽  
Fernanda C. Basso ◽  
Flávia B. de Assis ◽  
Fernando A. Souza ◽  
Telma T. Berchielli ◽  
...  
Keyword(s):  
Organic Matter ◽  
Bacillus Subtilis ◽  
Chemical Composition ◽  
Lactobacillus Plantarum ◽  
Corn Silage ◽  
In Vitro Digestibility ◽  
Aerobic Stability ◽  
Organic Matter Digestibility ◽  
Corn Plants

Chemical composition, fermentation characteristics, in vitro digestibility and aerobic stability were evaluated in corn silage inoculated with microbial additives in two different experiments. Inoculant treatments (untreated, Bacillus subtilis and B. subtilis combined with Lactobacillus plantarum) were applied to fresh forages. Chopped corn plants (2B655 Hx) were ensiled in laboratory silos for periods of 7, 14, 21 and 63 days to evaluate the fermentation parameters. The experimental silos were weighed to determine gas losses. After the ensiling period, the silage was sampled to determine chemical composition and in vitro organic matter digestibility. To evaluate aerobic stability, chopped corn plants (AG‐1051) were ensiled in laboratory silos that were opened after 96 days of ensiling. The silage was placed in different buckets containing data loggers. The silage was sampled after 0, 4, 8 and 12 days of exposure to air to evaluate the microbial populations and pH. The data were analysed as a completely randomised design using a mixed repeated-measures model in the MIXED procedure of SAS. To evaluate each treatment relative to the fermentation times, a regression analysis using the PROC REG procedure of SAS was applied. A significance level of P < 0.05 was used. Inoculation with both strains increased lactic acid concentration, whereas the use of B. subtilis alone or combined with L. plantarum improved in vitro apparent organic matter digestibility. In the B. subtilis and B. subtilis combined with L. plantarum silages, moulds and yeasts decreased, and aerobic stability was improved. Inoculation with B. subtilis alone or combined with L. plantarum improved the nutritional value and aerobic stability of corn silage.

scholarly journals Murumuru (Astrocaryum murumuru) meal as an additive to elephant grass silage

2020 ◽  
Vol 33 (4) ◽  
pp. 264-272
Author(s):  
Cláudia M Serra-Ferreira ◽  
Agatha G Farias-Souza ◽  
Rita C Almeida-Mendonça ◽  
Melany Simões-Souza ◽  
Wagner R L Lopes-Filho ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Moisture Content ◽  
Dry Matter ◽  
Matter Content ◽  
Dry Matter Content ◽  
Elephant Grass ◽  
High Moisture Content ◽  
High Moisture ◽  
Grass Silage ◽  
Aerobic Stability

Background: Tropical grasses, such as elephant grass, have high moisture content during its ideal phenological state for silage. High moisture content hinders proper preservation and reduces the nutritive value of silage due to secondary fermentation and production of effluents. Adding feed materials with high dry matter content, such as murumuru (Astrocaryum murumuru) meal, is a potential alternative to improve silage yield. Objective: To determine the effects of including murumuru meal (0, 7, 14, 21, and 28%) on the fermentative characteristics, microbiological activity, aerobic stability, and chemical composition of elephant grass silages. Methods: A completely randomized design with five treatments and five replicates was used. Elephant grass was collected at 60 d of age, minced, and homogenized with murumuru meal. The mass was placed in experimental 15-L silos. The silos were collected and analyzed 45 d later. Results: Effluent production decreased (p<0.05) as the proportions of murumuru meal in silage increased. A quadratic effect (p<0.05) was observed on dry matter recovery. An increase (p<0.05) was observed in dry matter content, a decrease (p<0.05) in the neutral detergent fiber content, and an increase (p<0.05) in the non-fibrous carbohydrate content with the inclusion of murumuru meal. Conclusions: Addition of murumuru meal improves chemical composition and does not affect the fermentative characteristics of elephant grass silage, while it reduces effluent losses. Nevertheless, the inclusion of murumuru meal in the elephant grass silage decreased the time of aerobic stability.

Meta-analysis of the effects of Lactobacillus plantarum and Lactobacillus buchneri on fermentation, chemical composition and aerobic stability of sugarcane silage

2018 ◽  
Vol 65 (1) ◽  
pp. 3-12
Author(s):  
Carlos Henrique Silveira Rabelo ◽  
Carla Joice Härter ◽  
Carla Luiza da Silva Ávila ◽  
Ricardo Andrade Reis
Keyword(s):  
Chemical Composition ◽  
Lactobacillus Plantarum ◽  
Meta Analysis ◽  
Lactobacillus Buchneri ◽  
Aerobic Stability

scholarly journals High-moisture corn grain silage inoculated with Propionibacterium acidipropionici and Lactobacillus plantarum in different storage times

2021 ◽  
Vol 10 (1) ◽  
pp. e0410110371
Author(s):  
Rafael Frank ◽  
Marcela Abbado Neres ◽  
Tatiane Fernandes ◽  
Rodrigo André Schöne ◽  
André Sanches de Avila ◽  
...  
Keyword(s):  
Lactobacillus Plantarum ◽  
High Moisture ◽  
Propionibacterium Acidipropionici ◽  
Colony Forming Unit ◽  
Ph Values ◽  
Aerobic Stability ◽  
Storage Times ◽  
And Storage ◽  
Corn Grain ◽  
High Moisture Corn

The aim of this study was to evaluate the chemical composition, growth of microorganisms, and the aerobic stability of high-moisture corn grain silage inoculated with Lactobacillus plantarum + Propionibacterium acidipropionici. The experimental design was completely randomized in a 2 x 5 factorial arrangement with four replications (50 experimental units)  and the treatments were: use or not of microbial inoculant (2 x 105 colony forming unit (CFU) g-1 Lactobacillus plantarum + Propionibacterium acidipropionici) and the storage length for 0, 1, 3, 7, and 14 d. Aerobic stability of silage was evaluated at 28 and 56 d of storage length. The lactic acid bacteria population was influenced by storage length and the greatest values were estimated at 8 d of storage length. Regardless of inoculant application, no enterobacteria were present from 3 d post-ensiling. With respect to mold growth, an interaction between inoculant and storage length was observed wherein molds were most abundant after 3 d of storage in silage that received inoculant. However, at 14 d of storage the use of bacterial inoculant reduced the occurrence of molds. pH values obtained after 3 d of ensiling were less than 4.0 for all the treatments. The high-moisture corn grain silage possessed good fermentative quality. Further, adequate pH values were achieved from the third day of ensilage and were not influenced by the presence of bacterial inoculant.

scholarly journals Application of Selected Inoculant Producing Antifungal and Fibrinolytic Substances on Rye Silage with Different Wilting Time

Processes ◽  
2021 ◽  
Vol 9 (5) ◽  
pp. 879
Author(s):  
Seong-Shin Lee ◽  
Jeong-Seok Choi ◽  
Dimas Hand Vidya Paradhipta ◽  
Young-Ho Joo ◽  
Hyuk-Jun Lee ◽  
...  
Keyword(s):  
Lactobacillus Plantarum ◽  
Dry Matter ◽  
Lactobacillus Brevis ◽  
Neutral Detergent Fiber ◽  
Dry Matter Digestibility ◽  
Fiber Digestibility ◽  
Aerobic Stability ◽  
Commercial Inoculant

This research was conducted to determine the effects of selected inoculant on the silage with different wilting times. The ryes were unwilted or wilted for 12 h. Each rye forage was ensiled for 100 d in quadruplicate with commercial inoculant (Lactobacillus plantarum sp.; LPT) or selected inoculant (Lactobacillus brevis 100D8 and Leuconostoc holzapfelii 5H4 at 1:1 ratio; MIX). In vitro dry matter digestibility and in vitro neutral detergent fiber digestibility were highest in the unwilted MIX silages (p < 0.05), and the concentration of ruminal acetate was increased in MIX silages (p < 0.001; 61.4% vs. 60.3%) by the increase of neutral detergent fiber digestibility. The concentration of ruminal ammonia-N was increased in wilted silages (p < 0.001; 34.8% vs. 21.1%). The yeast count was lower in the MIX silages than in the LPT silages (p < 0.05) due to a higher concentration of acetate in MIX silages (p < 0.05). Aerobic stability was highest in the wilted MIX silages (p < 0.05). In conclusion, the MIX inoculation increased aerobic stability and improved fiber digestibility. As a result of the wilting process, ammonia-N in silage decreased but ruminal ammonia-N increased. Notably, the wilted silage with applied mixed inoculant had the highest aerobic stability.

scholarly journals Effect of Substitution of Urea With Different Type and Levels of Ruminant Manure on Nutritive Value of Rice Straw Silage

2019 ◽  
Vol 9 (2) ◽  
pp. 206-214
Author(s):  
Ali Ameen Saeed ◽  
Saja Intisar Abid
Keyword(s):  
Organic Matter ◽  
Rice Straw ◽  
Dry Matter ◽  
Nutritive Value ◽  
In Vitro Digestibility ◽  
N Content ◽  
Aerobic Stability

This study was conducted in Nutrition Lab. to investigate the effect of the type and level of substitution of urea with ruminant manure, M (sheep, cow and buffalo) on basis of nitrogen (N) content on the nutritive value of rice straw silage (RSS). Accordingly silages were nominated as, S-RSS, C-RSS and B-RSS. Urea (U) was substituted with dried manure at 6 combinations, 100:0, 90:10, 80:20, 70:30, 60:40 and 50:50 of U:M. Silage samples were prepared by treating chopped straw with pre-treated solution contained 10% low quality debis and 2% urea. Results showed that lower (P˂0.01) DM loss (11.4%) was observed in S-RSS, and with addition of urea only (3.6%).        Samples of S-RSS and C-RSS recorded higher (P˂0.01) Fleig points (Fp) as compared with those prepared by the addition of B-RSS, 60.42, 55.58 and 49.59 respectively. Reduction (P˂0.01) in this parameter was noticed in samples prepared with a combination of 100:0. Aerobic stability (AS) was a reduced (P˂0.01) in samples prepared by addition of S-RSS by 15 and 13 hours in comparison with samples of C-RSS and B-RSS respectively. Samples prepared with combination of 100:0 were prior (P˂0.01) as compared with other samples.        Results also showed an increase (P˂0.01) in in vitro digestibility of organic matter (IVOMD) in samples of S-RSS in comparison with samples prepared by addition of C-RSS and B-RSS, 49.99, 44.59 and 42.77% respectively. Samples prepared with combination of 100:0 recorded lower (P˂0.05) in vitro digestibility of dry matter (IVDMD) as compared with combinations of 70:30 and 60:40 of U: M, 40.52, 45.36 and 45.94% respectively.

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